Horse milk powder capable of improving intestinal health and preparation technology of horse milk powder

Abstract

The invention belongs to the technical field of food processing, and provides horse milk powder for improving intestinal health and a preparation technology thereof. The horse milk powder capable of improving intestinal health is prepared from the following components in parts by weight: 60 to 70 parts of whole horse milk powder, 3.0 to 4.0 parts of galactooligosaccharide, 4.0 to 5.0 parts of lactulose, 3.5 to 4.5 parts of beta-glucan, 1.5 to 2.5 parts of inulin, 5.0 to 6.0 parts of complex probiotics, 2.5 to 3.5 parts of pachymaran, 6.5 to 7.5 parts of astragalus polysaccharide and 5.0 to 6.0 parts of calcium carbonate. According to the horse milk powder capable of improving intestinal health disclosed by the invention, medicinal and edible components are added, and probiotics and various prebiotics are compounded, so that the activities of DAO, D-LAC and MPO in intestinal tissues can be remarkably reduced, the contents of acetic acid, propionic acid and butyric acid are increased, and the barrier function of the intestinal tract is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing and relates to mare's milk powder for improving intestinal health and a preparation process thereof. Background Art

[0002] The intestine is the primary site for digestion and nutrient absorption in the human body and is also the body's largest immune organ. Acting as an innate barrier, the intestine maintains internal environmental balance, preventing the invasion of non-self-harmful antigens, bacteria, and viruses, and reducing systemic crosstalk between intestinal microbes.

[0003] The intestinal barrier is a complex system composed of the intestinal mucosa, intestinal epithelial cells, immune cells, and intestinal microbes. It is closely related to body dysfunction and occupies a central position in intestinal health. Intestinal barrier dysfunction is considered a key factor in the development of various pathophysiological conditions. A damaged barrier leads to increased intestinal permeability, allowing external antigens to invade the body, disrupting microbial homeostasis and inducing a systemic inflammatory response. By strengthening tight junctions between intestinal cells, the production of intestinal mucus, antimicrobial proteins, and immune cells can be induced, and improving the intestinal microbiome can repair a damaged intestinal barrier.

[0004] Fresh mare's milk is purified, concentrated, and spray-dried to produce mare's milk powder, transforming liquid milk into powder. This facilitates packaging and transportation, extending the shelf life of mare's milk products while retaining the unique nutritional value and health benefits of mare's milk. Mare's milk powder has significant antioxidant activity and also has certain anti-fatigue and immunomodulatory effects.

[0005] Currently, there is still a lack of full-fat mare's milk powder suitable for people with damaged intestinal barriers on the market. This is an urgent problem to be solved by technicians in this field. The development of a mare's milk powder that can improve intestinal health has broad application and market prospects. Summary of the Invention

[0006] In view of this, the present invention provides a mare's milk powder for improving intestinal health and a preparation process thereof. The mare's milk powder for improving intestinal health of the present invention is supplemented with medicinal and edible ingredients, compounded with probiotics and multiple prebiotics. The mare's milk powder of the present invention significantly reduces the activities of DAO, D-LAC, and MPO in intestinal tissues, increases the levels of acetic acid, propionic acid, and butyric acid, improves intestinal barrier function, helps the body absorb nutrients, and enhances immunity.

[0007] The mare's milk powder for improving intestinal health of the present invention is prepared from the following components by weight: 60-70 parts of whole mare's milk powder, 3.0-4.0 parts of galacto-oligosaccharides, 4.0-5.0 parts of lactulose, 3.5-4.5 parts of beta-glucan, 1.5-2.5 parts of inulin, 5.0-6.0 parts of compound probiotics, 2.5-3.5 parts of tuckahoe polysaccharide, 6.5-7.5 parts of astragalus polysaccharide and 5.0-6.0 parts of calcium carbonate; The composite probiotics consist of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in a weight ratio of 1:1.0-2.5:0.8-1.5:0.5-1.3.

[0008] In one embodiment of the present invention, the mare's milk powder for improving intestinal health is made of the following components by weight: 65 parts of whole mare's milk powder, 3.5 parts of galactoligosaccharides, 4.5 parts of lactulose, 4.0 parts of β-glucan, 2.0 parts of inulin, 5.5 parts of compound probiotics, 3.0 parts of Poria polysaccharide, 7.0 parts of astragalus polysaccharide and 5.5 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:2:1:0.8.

[0009] In one embodiment of the present invention, the mare's milk powder for improving intestinal health is made of the following components by weight: 60 parts of whole mare's milk powder, 4.0 parts of galactoligosaccharides, 4.0 parts of lactulose, 3.5 parts of β-glucan, 1.5 parts of inulin, 6.0 parts of compound probiotics, 3.5 parts of Poria polysaccharide, 7.5 parts of astragalus polysaccharide and 6.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:2.5:0.8:1.3.

[0010] In one embodiment of the present invention, the mare's milk powder for improving intestinal health is made of the following components by weight: 70 parts of whole mare's milk powder, 3.0 parts of galactoligosaccharides, 5.0 parts of lactulose, 4.5 parts of β-glucan, 2.5 parts of inulin, 5.0 parts of compound probiotics, 2.5 parts of Poria polysaccharide, 6.5 parts of astragalus polysaccharide and 5.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:1:1:1.

[0011] In one embodiment of the present invention, the mare's milk powder for improving intestinal health is made of the following components by weight: 65 parts of whole mare's milk powder, 4.0 parts of galactoligosaccharides, 5.0 parts of lactulose, 3.5 parts of β-glucan, 1.5 parts of inulin, 6.0 parts of compound probiotics, 3.0 parts of Poria polysaccharide, 6.5 parts of astragalus polysaccharide and 6.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:2.5:0.8:1.3.

[0012] In one embodiment of the present invention, the mare's milk powder for improving intestinal health is made of the following components by weight: 70 parts of whole mare's milk powder, 3.5 parts of galactoligosaccharides, 4.0 parts of lactulose, 3.5 parts of β-glucan, 2.5 parts of inulin, 5.5 parts of compound probiotics, 2.5 parts of Poria polysaccharide, 7.0 parts of astragalus polysaccharide and 5.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:1:1.5:1.3.

[0013] The present invention also provides a method for preparing the mare's milk powder for improving intestinal health, comprising the following steps: crushing whole mare's milk powder, galacto-oligosaccharide, lactulose, β-glucan, inulin, pachymaran, astragalus polysaccharide and calcium carbonate respectively, sieving, weighing the above components by weight, placing them into a sterilized mixing container, mixing them evenly, drying them, and then adding composite probiotics to obtain the mare's milk powder for improving intestinal health.

[0014] The present invention also provides use of the mare's milk powder for improving intestinal health in preparing a product for improving intestinal barrier function.

[0015] The present invention also provides use of the mare's milk powder for improving intestinal health in preparing a product for improving intestinal function in people with irritable bowel syndrome.

[0016] Poria cocos, an important traditional Chinese medicine, has demonstrated its efficacy in clinical and biological studies. Pharmacological studies have shown that polysaccharides, the most abundant substance in Poria cocos, possess a wide range of biological activities, including anti-tumor, immunomodulatory, anti-inflammatory, antioxidant, anti-aging, anti-hepatitis, anti-diabetic, and anti-hemorrhagic fever effects. Animal studies have shown that Poria cocos polysaccharides can improve intestinal mucosal integrity and modulate the intestinal microbiome to alleviate hyperglycemia and hyperlipidemia in mice.

[0017] Astragalus root is warm in nature and sweet in flavor. It enters the lung and spleen meridians and has the effects of tonifying qi and raising yang, strengthening the exterior and stopping perspiration, detoxifying, promoting diuresis, reducing swelling, and promoting tissue regeneration. It is one of the most commonly used qi-tonifying herbs in clinical practice. Astragalus root can bidirectionally regulate the body's immune function, promoting interferon production in immune cells, delaying cell aging, providing antibacterial and fatigue-fighting properties, and enhancing the body's adaptability and disease resistance. Astragalus polysaccharide, one of the main active ingredients in astragalus root, has therapeutic effects on colitis, associated with reducing intestinal inflammation, promoting mucosal repair, and improving mucosal barrier function.

[0018] Prebiotics such as galacto-oligosaccharides, lactulose, beta-glucan, and inulin nourish specific microorganisms in the digestive tract, promoting the growth of beneficial bacteria over harmful ones. Probiotics are used to support the body's natural intestinal microbiome, and some probiotic preparations have been used to prevent antibiotic-induced diarrhea or as part of the treatment of antibiotic-associated dysbiosis. Studies have shown that probiotics are effective for a variety of gastrointestinal and extra-gastrointestinal conditions, including inflammatory bowel disease, irritable bowel syndrome, vaginal infections, and immune enhancement.

[0019] Compared with the prior art, the present invention has the following advantages: DAO, D-LAC, and MPO are key indicators of intestinal barrier function. Increased levels in small intestinal tissue indicate damage to the intestinal mucosal barrier. The mare's milk powder of the present invention significantly reduces the activity of DAO, D-LAC, and MPO in intestinal tissue, thereby improving intestinal barrier function.

[0020] The mare's milk powder for improving intestinal health of the present invention significantly increases the contents of acetic acid, propionic acid and butyric acid, further confirming the efficacy of the mare's milk powder for regulating intestinal barrier function.

[0021] After reducing the components of the mare's milk powder of the present invention and changing the types of composite probiotics, the effect of improving the intestinal barrier function was significantly weakened, indicating that the raw materials of the mare's milk powder for improving intestinal health of the present invention exert a synergistic effect, and the prepared mare's milk powder has a good effect of improving the intestinal barrier function.

[0022] The intestinal barrier separates the intestinal cavity from the internal environment of the body and plays an important role in preventing and stopping systemic inflammatory reactions and the immune system. The mare's milk powder of the present invention improves intestinal barrier dysfunction, helps the body absorb nutrients, and enhances immunity, and has broad development potential. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0024] Figure 1 Comparison of DAO content among mice groups. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0025] Figure 2 Comparison of D-LAC content among mice in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0026] Figure 3Comparison of MPO levels in mice of each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05). DETAILED DESCRIPTION

[0027] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0028] The probiotics used in the present invention: Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus, can be selected from commercially available common strains.

[0029] The whole-fat mare's milk powder of the present invention is prepared by a conventional process. Specifically, the whole-fat mare's milk powder in the following Examples 1-6 and Comparative Examples 1-5 is prepared by the following method: raw mare's milk is placed in a homogenizer for homogenous mixing, the homogenization mixing process is carried out under the conditions of 20° C. and 15 MPa, and then sterilized at 80° C. for 5 minutes and freeze-dried at -70° C. for 15 hours to obtain the whole-fat mare's milk powder.

[0030] The preparation method of the Poria cocos polysaccharide used in the present invention is as follows: drying the Poria cocos slices at 60°C, crushing them, passing them through an 80-mesh sieve, weighing the Poria cocos powder, adding anhydrous ethanol according to a material-liquid ratio of 1:6, soaking for 36 hours, filtering, and drying to obtain defatted Poria cocos powder, weighing 10g of the defatted Poria cocos powder, adding 150mL of ultrapure water to dissolve it, extracting it in a 90°C water bath, centrifuging, taking the supernatant, extracting it three times, combining the extracts, centrifuging them at 4000rpm for 15 minutes, collecting the supernatant and concentrating it to 1 / 8 of the original volume, then adding 3 times the volume of anhydrous ethanol to the polysaccharide concentrate, quickly mixing it, precipitating it with alcohol at 4°C overnight, centrifuging it, collecting the precipitate, and freeze-drying it to obtain the Poria cocos polysaccharide.

[0031] The preparation method of the astragalus polysaccharide used in the present invention is as follows: astragalus slices are dried at 60°C, crushed, and passed through an 80-mesh sieve, astragalus powder is weighed, anhydrous ethanol is added according to a material-liquid ratio of 1:9, the mixture is soaked for 45 hours, filtered, and dried to obtain defatted astragalus powder, 20g of the defatted astragalus powder is weighed, 400mL of ultrapure water is added for dissolution, extraction is performed in a 90°C water bath, centrifugation is performed, the supernatant is taken, extraction is performed three times, the extracts are combined, centrifuged at 4000rpm for 25 minutes, the supernatant is collected and concentrated to 1 / 12 of the original volume, and then 5 times the volume of anhydrous ethanol is added to the polysaccharide concentrate, the mixture is quickly mixed, the mixture is precipitated at 4°C overnight, centrifuged, the precipitate is collected, and freeze-dried to obtain astragalus polysaccharide.

[0032] Example 1 A mare's milk powder for improving intestinal health and its preparation process 65 parts of full-fat mare's milk powder, 3.5 parts of galacto-oligosaccharides, 4.5 parts of lactulose, 4.0 parts of β-glucan, 2.0 parts of inulin, 5.5 parts of compound probiotics, 3.0 parts of Poria polysaccharide, 7.0 parts of astragalus polysaccharide and 5.5 parts of calcium carbonate.

[0033] The weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the composite probiotics is 1:2:1:0.8.

[0034] Preparation method: full-fat mare's milk powder, galacto-oligosaccharide, lactulose, β-glucan, inulin, pachymaran, astragalus polysaccharide and calcium carbonate are crushed and sieved respectively, and the above components are weighed according to weight, put into a sterilized mixing container, mix evenly, and dry, and then add compound probiotics to obtain full-fat mare's milk powder.

[0035] Example 2 A mare's milk powder for improving intestinal health and its preparation process 60 parts of full-fat mare's milk powder, 4.0 parts of galacto-oligosaccharides, 4.0 parts of lactulose, 3.5 parts of β-glucan, 1.5 parts of inulin, 6.0 parts of compound probiotics, 3.5 parts of Poria polysaccharide, 7.5 parts of astragalus polysaccharide and 6.0 parts of calcium carbonate.

[0036] The weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the composite probiotics is 1:2.5:0.8:1.3.

[0037] The preparation method is as in Example 1.

[0038] Example 3 A mare's milk powder for improving intestinal health and its preparation process 70 parts of full-fat mare's milk powder, 3.0 parts of galacto-oligosaccharide, 5.0 parts of lactulose, 4.5 parts of β-glucan, 2.5 parts of inulin, 5.0 parts of compound probiotics, 2.5 parts of Poria polysaccharide, 6.5 parts of astragalus polysaccharide and 5.0 parts of calcium carbonate.

[0039] The weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the composite probiotics is 1:1:1:1.

[0040] The preparation method is as in Example 1.

[0041] Example 4 A mare's milk powder for improving intestinal health and its preparation process 65 parts of full-fat mare's milk powder, 4.0 parts of galacto-oligosaccharides, 5.0 parts of lactulose, 3.5 parts of β-glucan, 1.5 parts of inulin, 6.0 parts of compound probiotics, 3.0 parts of Poria polysaccharide, 6.5 parts of astragalus polysaccharide and 6.0 parts of calcium carbonate.

[0042] The weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the composite probiotics is 1:2.5:0.8:1.3.

[0043] The preparation method is as in Example 1.

[0044] Example 5 A mare's milk powder for improving intestinal health and its preparation process 70 parts of full-fat mare's milk powder, 3.5 parts of galacto-oligosaccharides, 4.0 parts of lactulose, 3.5 parts of β-glucan, 2.5 parts of inulin, 5.5 parts of compound probiotics, 2.5 parts of Poria polysaccharide, 7.0 parts of astragalus polysaccharide and 5.0 parts of calcium carbonate.

[0045] The weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the composite probiotics is 1:1:1.5:1.3.

[0046] The preparation method is as in Example 1.

[0047] Example 6 A mare's milk powder for improving intestinal health and its preparation process 60 parts of full-fat mare's milk powder, 3.-4.0 parts of galacto-oligosaccharides, 4.5 parts of lactulose, 4.0 parts of β-glucan, 2.5 parts of inulin, 5.0 parts of compound probiotics, 3.5 parts of Poria polysaccharide, 6.5 parts of astragalus polysaccharide and 5.5 parts of calcium carbonate.

[0048] The weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the composite probiotics is 1:2.5:0.8:1.3.

[0049] The preparation method is as in Example 1.

[0050] Comparative Example 1 65 parts of full-fat mare's milk powder, 4.0 parts of beta-glucan, 2.0 parts of inulin, 5.5 parts of compound probiotics, 3.0 parts of tuckahoe polysaccharide, 7.0 parts of astragalus polysaccharide and 5.5 parts of calcium carbonate.

[0051] Compared with Example 1, the difference is that galacto-oligosaccharide and lactulose are not contained, and the proportions of other components remain unchanged.

[0052] The preparation method is as in Example 1.

[0053] Comparative Example 2 65 parts of full-fat mare's milk powder, 3.5 parts of galacto-oligosaccharides, 4.5 parts of lactulose, 5.5 parts of compound probiotics, 3.0 parts of tuckahoe polysaccharides, 7.0 parts of astragalus polysaccharides and 5.5 parts of calcium carbonate.

[0054] Compared with Example 1, the difference is that β-glucan and inulin are not contained, and the proportions of other components remain unchanged.

[0055] The preparation method is as in Example 1.

[0056] Comparative Example 3 65 parts of full-fat mare's milk powder, 3.5 parts of galacto-oligosaccharide, 4.5 parts of lactulose, 4.0 parts of β-glucan, 2.0 parts of inulin, 3.0 parts of tuckahoe polysaccharide, 7.0 parts of astragalus polysaccharide and 5.5 parts of calcium carbonate.

[0057] Compared with Example 1, the difference is that the compound probiotics are not contained, and the proportions of other components remain unchanged.

[0058] The preparation method is as in Example 1.

[0059] Comparative Example 4 65 parts of full-fat mare's milk powder, 3.5 parts of galacto-oligosaccharides, 4.5 parts of lactulose, 4.0 parts of β-glucan, 2.0 parts of inulin, 5.5 parts of compound probiotics, 3.0 parts of Poria polysaccharide, 7.0 parts of astragalus polysaccharide and 5.5 parts of calcium carbonate.

[0060] Compared with Example 1, the difference is that the composition of the composite probiotics is changed, and the proportions of other components remain unchanged.

[0061] The weight ratio of Lactobacillus helveticus to Lactobacillus bulgaricus in the composite probiotics is 3:2.8.

[0062] The preparation method is as in Example 1.

[0063] Comparative Example 5 65 parts of full-fat mare's milk powder, 3.5 parts of galacto-oligosaccharides, 4.5 parts of lactulose, 4.0 parts of β-glucan, 2.0 parts of inulin, 5.5 parts of compound probiotics and 5.5 parts of calcium carbonate.

[0064] Compared with Example 1, the difference is that Poria polysaccharide and Astragalus polysaccharide are not contained, and the proportions of other components remain unchanged.

[0065] The preparation method is as in Example 1.

[0066] Experimental Example 1 Study on the effect of the mare's milk powder for improving intestinal health on intestinal barrier permeability 1. Experimental Animals and Drug Administration 72 21-day-old healthy and well-growing male Kunming mice were randomly divided into groups of 8 mice each, namely, the control group, Example 1 group (administered 7.6 g / kg mare's milk powder of Example 1), Example 2 group (administered 7.6 g / kg mare's milk powder of Example 2), Example 3 group (administered 7.6 g / kg mare's milk powder of Example 3), Comparative Example 1 group (administered 7.6 g / kg mare's milk powder of Comparative Example 1), Comparative Example 2 group (administered 7.6 g / kg mare's milk powder of Comparative Example 2), Comparative Example 3 group (administered 7.6 g / kg mare's milk powder of Comparative Example 3), Comparative Example 4 group (administered 7.6 g / kg mare's milk powder of Comparative Example 4), and Comparative Example 5 group (administered 6.45 g / kg mare's milk powder of Comparative Example 5).

[0067] Mice in each group had free access to water and food, the room temperature was 22-26°C, and the light-dark cycle was 12:12 daily. Groups 1-3 of Examples and groups 1-5 of Comparative Examples were gavaged with the corresponding mare's milk powder, while the control group was gavaged with an equal volume of normal saline at a dose of 10 mL / kg, once daily for 25 consecutive days.

[0068] 2. Index detection 2.1 Determination of intestinal barrier markers On day 25 of the experimental period, mice were fasted overnight and sacrificed by cervical dislocation. Small intestinal samples were collected from Kunming mice, and the intestinal mucosal layer was isolated for barrier permeability testing. D-LAC levels in mouse small intestinal tissue were measured using an ELISA kit, DAO was measured using an immunocolorimetric assay, and MPO was measured using a colorimetric assay. These indicators were tested according to the kit instructions.

[0069] 3. Statistical methods Graphpad Prism 7.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation. The comparison among multiple groups was performed by one-way analysis of variance, and the comparison between groups was performed by SNK-q. The difference was considered statistically significant when P < 0.05.

[0070] 4. Experimental Results Effects on intestinal barrier markers in mouse small intestinal tissue Compared with the control group, the intestinal barrier markers DAO activity, D-LAC level and MPO activity of the small intestinal tissue of mice in each group of the embodiment were significantly reduced. Compared with the comparative example 1-5 groups, the groups of the embodiment had significant differences in reducing DAO activity, D-LAC level and MPO activity. Figure 1 、 Figure 2 、 Figure 3 .

[0071] Increased levels of DAO, D-LAC, and MPO in small intestinal tissue, key markers of the intestinal barrier, indicate damage to the intestinal mucosal barrier. The mare's milk powder of the present invention significantly reduced the activity of DAO, D-LAC, and MPO in the intestinal tissues of mice, improving intestinal barrier function. The improvement in intestinal barrier function in Examples 1-3 was significantly superior to that in Comparative Examples 1-5.

[0072] In comparative examples 1-5, after reducing the components and changing the types of composite probiotics in the mare's milk powder, the effect of improving the intestinal barrier function was significantly weakened, indicating that the raw materials of the mare's milk powder of the present invention exert a synergistic effect, and the prepared mare's milk powder has a good effect of improving the intestinal barrier function.

[0073] Experimental Example 2 Study on the effect of full-fat mare's milk powder of the present invention on mice with irritable bowel syndrome 1. Establishment of Irritable Bowel Syndrome Model in Mice 80 SPF-grade C57BL / 6J mice, half male and half female, were randomly selected as blank control groups, and the remaining mice were divided into 9 groups according to the random number method, with 8 mice in each group, namely, model control group, Example 1 group (administered 7.6 g / kg mare's milk powder of Example 1), Example 2 group (administered 7.6 g / kg mare's milk powder of Example 2), Example 3 group (administered 7.6 g / kg mare's milk powder of Example 3), Comparative Example 1 group (administered 7.6 g / kg mare's milk powder of Comparative Example 1), Comparative Example 2 group (administered 7.6 g / kg mare's milk powder of Comparative Example 2), Comparative Example 3 group (administered 7.6 g / kg mare's milk powder of Comparative Example 3), Comparative Example 4 group (administered 7.6 g / kg mare's milk powder of Comparative Example 4), and Comparative Example 5 group (administered 7.6 g / kg mare's milk powder of Comparative Example 5).

[0074] An irritable bowel syndrome model was established using sodium dextran sulfate. All mice, except the blank control group, were given a 3% (w / v) solution of sodium dextran sulfate (molecular weight 36,000-50,000) in drinking water for 8 days. The blank control group was given regular drinking water for the same period. The modeling mice showed significant weight differences on day 6, with a downward trend in weight. They also developed symptoms such as decreased appetite, emaciation, and vomiting, suggesting an inflammatory response in the intestines of the model mice.

[0075] After modeling, the mice in Example 1-3 and Comparative Example 1-5 groups were gavaged with the corresponding mare's milk powder. The mice in the blank control group and model control group were gavaged with an equal volume of normal saline at a dose of 10 mL / kg once daily for 16 consecutive days. After gavage, the mice were processed, samples were collected, and indicators were recorded.

[0076] 2. Index detection Gas chromatography detection method for the determination of acetic acid, propionic acid and butyric acid Standard detection: Prepare a standard solution with 1 mg / mL butyronitrile as the internal standard. After dilution 20,000 times, calculate the detection concentration based on the ratio of the butyronitrile peak area to the peak area of ​​each acid.

[0077] Sample pretreatment: Mouse fecal samples were prepared with distilled water to a concentration of 100 mg / mL. After centrifugation, the supernatant was collected. The activated polypyrrole nanofibers were extracted from the supernatant and eluted with 100 μL of ethanol containing 0.01 mol / L hydrochloric acid. After mixing, the assay was initiated.

[0078] Detection chromatographic conditions: inlet temperature 250 °C, initial temperature 50 °C, 1 min, 10 °C / min to 180 °C, 2 min, total 10 min.

[0079] 3. Statistical methods Graphpad Prism 7.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation. The comparison among multiple groups was performed by one-way analysis of variance, and the comparison between groups was performed by SNK-q. The difference was considered statistically significant when P < 0.05.

[0080] 4. Experimental Results Acetic, propionic and butyric acid content Compared with the blank control group, the contents of acetic acid, propionic acid and butyric acid in the feces of mice in the model control group were significantly reduced.

[0081] Compared with the model control group, the levels of acetic acid, propionic acid, and butyric acid in the feces of mice in each of the Example groups were significantly increased. Compared with the Comparative Example groups 1-5, the levels of acetic acid, propionic acid, and butyric acid in the feces of mice in each of the Example groups were significantly different. The results are shown in Table 1. Note: Different lowercase letters in the same column indicate significant differences among different groups (P < 0.05).

[0082] After administering the mare's milk powder of the present invention to mice with irritable bowel syndrome model, the contents of acetic acid, propionic acid, and butyric acid in the mouse feces significantly increased, suggesting that the mare's milk powder of the present invention can regulate the intestinal barrier function of mice with irritable bowel syndrome and reduce damage to the mouse intestine.

[0083] In comparative examples 1-5, after reducing the components and changing the types of composite probiotics in the mare's milk powder, the intervention effect on the irritable bowel syndrome model mice was significantly weakened, indicating that the raw materials of the mare's milk powder of the present invention exert a synergistic effect, and the prepared mare's milk powder can regulate the intestinal barrier function and reduce damage to the intestine.

[0084] The above embodiments do not limit the present invention in any form. For those skilled in the art, any modifications, replacements, etc. made within the scope of the technical solution of the present invention based on the technical essence of the present invention still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A mare's milk powder for improving intestinal health, characterized in that: The mare's milk powder for improving intestinal health is prepared from the following components by weight: 60-70 parts of whole mare's milk powder, 3.0-4.0 parts of galacto-oligosaccharide, 4.0-5.0 parts of lactulose, 3.5-4.5 parts of β-glucan, 1.5-2.5 parts of inulin, 5.0-6.0 parts of compound probiotics, 2.5-3.5 parts of tuckahoe polysaccharide, 6.5-7.5 parts of astragalus polysaccharide and 5.0-6.0 parts of calcium carbonate; The composite probiotics consist of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in a weight ratio of 1:1.0-2.5:0.8-1.5:0.5-1.

3.

2. The mare milk powder for improving intestinal health according to claim 1, characterized in that: The mare's milk powder for improving intestinal health is prepared from the following components by weight: 65 parts of whole mare's milk powder, 3.5 parts of galacto-oligosaccharide, 4.5 parts of lactulose, 4.0 parts of β-glucan, 2.0 parts of inulin, 5.5 parts of compound probiotics, 3.0 parts of Poria polysaccharide, 7.0 parts of astragalus polysaccharide and 5.5 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:2:1:0.

8.

3. The mare milk powder for improving intestinal health according to claim 1, characterized in that: The mare's milk powder for improving intestinal health is prepared from the following components by weight: 60 parts of whole mare's milk powder, 4.0 parts of galacto-oligosaccharide, 4.0 parts of lactulose, 3.5 parts of β-glucan, 1.5 parts of inulin, 6.0 parts of compound probiotics, 3.5 parts of Poria polysaccharide, 7.5 parts of astragalus polysaccharide and 6.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:2.5:0.8:1.

3.

4. The mare milk powder for improving intestinal health according to claim 1, characterized in that: The mare's milk powder for improving intestinal health is prepared from the following components by weight: 70 parts of whole mare's milk powder, 3.0 parts of galacto-oligosaccharide, 5.0 parts of lactulose, 4.5 parts of β-glucan, 2.5 parts of inulin, 5.0 parts of compound probiotics, 2.5 parts of Poria polysaccharide, 6.5 parts of astragalus polysaccharide and 5.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:1:1:

1.

5. The mare milk powder for improving intestinal health according to claim 1, characterized in that: The mare's milk powder for improving intestinal health is prepared from the following components by weight: 65 parts of whole mare's milk powder, 4.0 parts of galacto-oligosaccharide, 5.0 parts of lactulose, 3.5 parts of β-glucan, 1.5 parts of inulin, 6.0 parts of compound probiotics, 3.0 parts of Poria polysaccharide, 6.5 parts of astragalus polysaccharide and 6.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:2.5:0.8:1.

3.

6. The mare milk powder for improving intestinal health according to claim 1, characterized in that: The mare's milk powder for improving intestinal health is prepared from the following components by weight: 70 parts of whole mare's milk powder, 3.5 parts of galacto-oligosaccharide, 4.0 parts of lactulose, 3.5 parts of β-glucan, 2.5 parts of inulin, 5.5 parts of compound probiotics, 2.5 parts of Poria polysaccharide, 7.0 parts of astragalus polysaccharide and 5.0 parts of calcium carbonate; the weight ratio of Bifidobacterium longum, Lactobacillus helveticus, Lactobacillus casei and Lactobacillus bulgaricus in the compound probiotics is 1:1:1.5:1.

3.

7. The method for preparing the mare's milk powder for improving intestinal health according to any one of claims 1 to 6, characterized in that: The preparation method of the mare's milk powder for improving intestinal health comprises the following steps: respectively crushing and sieving whole mare's milk powder, galacto-oligosaccharide, lactulose, β-glucan, inulin, pachymaran, astragalus polysaccharide and calcium carbonate; weighing the above components by weight, placing them into a sterilized mixing container, mixing them evenly, and drying them; and then adding composite probiotics to obtain the mare's milk powder for improving intestinal health.

8. Use of the mare's milk powder for improving intestinal health according to claim 1 in the preparation of a product for improving intestinal barrier function.

9. Use of the mare's milk powder for improving intestinal health according to claim 1 in preparing a product for improving intestinal function in people with irritable bowel syndrome.

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