Skin-tightening anti-wrinkle essence and preparation method thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses skin-tightening and anti-wrinkle essence. The skin-tightening anti-wrinkle essence is prepared from the following raw materials in percentage by mass: 1.5 to 2.5 percent of acetyl hexapeptide-8, 2.0 to 3.0 percent of palmitoyl pentapeptide-4, 3.0 percent of hydroxypropyl tetrahydropyrantriol, 2.0 percent of lipidosome-coated vitamin C ethyl ether, 0.5 percent of resveratrol PLGA microspheres, 0.8 percent of ceramide NP, 1.2 percent of pachymaran, 5.0 percent of glyceryl polyether-26, 0.3 percent of sodium hyaluronate, 0.5 percent of p-hydroxyacetophenone and 1, 3-propanediol, 0.5 percent of sodium hyaluronate, 0.5 percent of sodium hyaluronate, 0.5 percent of sodium hyaluronate and the balance of water. The 1, 2-hexanediol accounts for 0.6%, and the balance is deionized water. Multi-target anti-wrinkle, deep moisturizing and long-acting repairing are achieved by scientifically compounding active ingredients such as acetyl hexapeptide-8 and palmitoyl pentapeptide-4 and combining a lipidosome wrapping technology and a nano-microsphere slow release technology, the preparation process comprises the steps of lipidosome homogenization, nano-microsphere multiple emulsion method and low-temperature emulsification, the ingredient stability and transdermal efficiency are ensured, and the skin care effect is good. Experiments prove that the essence can significantly improve skin elasticity and reduce wrinkles, and has a significant anti-aging effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a firming skin anti-wrinkle essence and a preparation method thereof. Background Art

[0002] With the acceleration of the natural aging process of the skin, the synthesis rate of type I and type III collagen in the dermis decreases by about 42% (with the inflection point at 35 years old). The enhanced activity of matrix metalloproteinases (MMPs) leads to the breakage of the collagen fiber network. At the same time, mitochondrial respiratory chain dysfunction causes the accumulation of excessive reactive oxygen species (ROS), resulting in lipid peroxidation of cell membranes and DNA damage. Eventually, it is manifested as a decrease in skin elasticity, sagging, and the formation of deep wrinkles. Existing anti-wrinkle products mostly rely on components with a single mechanism of action. For example, they only temporarily improve skin texture through moisturizers, or use unmodified vitamin C which has antioxidant properties but poor stability (the half-life in aqueous solution is only 2 hours), or rely on large-molecule hyaluronic acid to form a surface hydration film and it is difficult to penetrate the dermis layer. There is also a lack of means to intervene in dynamic wrinkles caused by excessive contraction of facial muscles. Such formulations often have poor compatibility between components, resulting in an unstable system. Active substances are easily inactivated during transdermal delivery due to the influence of pH value, ionic strength, and enzymatic hydrolysis, and the transdermal absorption rate is generally lower than 5%, making it difficult to reach an effective concentration at the action target. Therefore, this application has made improvements to solve this problem. Summary of the Invention

[0003] The purpose of the present invention is to provide a firming skin anti-wrinkle essence and a preparation method thereof to solve the problems raised in the above background art.

[0004] To achieve the above purpose, the present invention provides the following technical solution: A firming skin anti-wrinkle essence, which is composed of the following raw materials in mass percentage: acetyl hexapeptide-8 accounts for 1.5 - 2.5%, palmitoyl pentapeptide-4 accounts for 2.0 - 3.0%, hydroxypropyltetrahydropyrantriol accounts for 3.0%, vitamin C ethyl ether encapsulated in liposomes accounts for 2.0%, resveratrol PLGA microspheres account for 0.5%, ceramide NP accounts for 0.8%, pachyman polysaccharide accounts for 1.2%, glycerol polyether-26 accounts for 5.0%, sodium hyaluronate accounts for 0.3%, p-hydroxyacetophenone and 1,2-hexanediol account for 0.6%, and the balance is deionized water.

[0005] Preferably, the molar ratio of acetyl hexapeptide-8 to palmitoyl pentapeptide-4 is 1:1.3.

[0006] Preferably, the vitamin C ethyl ether encapsulated in liposomes is composed of hydrogenated lecithin and cholesterol in a ratio of 3:1, with a particle size ≤ 50 nm and an encapsulation efficiency ≥ 92%.

[0007] Preferably, the resveratrol PLGA microspheres have a particle size of 200 nm and a sustained release time of 72 hours.

[0008] Preferably, the preparation method comprises the following steps: Step 1, preparation of liposomes: Dissolve retinyl palmitate and vitamin C ethyl ether in a hydrogenated lecithin-cholesterol ethanol solution, and completely dissolve retinyl palmitate and vitamin C ethyl ether under constant temperature stirring conditions to form a homogeneous mixed solution; transfer the mixed solution to a rotary evaporator, and perform rotary evaporation to remove ethanol under appropriate temperature and vacuum conditions to concentrate the solution and preliminarily form liposomes; then transfer the product after rotary evaporation to a high-pressure homogenizer for high-pressure homogenization treatment to obtain a liposome suspension with uniform particle size and good dispersibility; Step 2, preparation of nano-microspheres: Dissolve resveratrol in an appropriate amount of organic solvent to form an oil-phase solution, and dissolve PLGA in another organic solvent to form a PLGA solution; slowly drop the resveratrol oil-phase solution into the PLGA solution under stirring conditions to form a water-in-oil (W / O) primary emulsion; slowly add the primary emulsion to an aqueous solution containing a surfactant to form a water-in-oil-in-water (W / O / W) multiple emulsion; volatilize the organic solvent and solidify PLGA under stirring conditions to form nano-microspheres; centrifuge the solidified nano-microsphere suspension to remove the supernatant, wash the nano-microspheres several times with deionized water to remove residual organic solvents and surfactants, and finally disperse the washed nano-microspheres in an appropriate amount of buffer solution for standby; Step 3, preparation of the aqueous phase: Under the condition of a 70°C constant temperature water bath, sequentially add glycerol polyether-26, sodium hyaluronate, and pachymaran to deionized water, and stir while adding until completely dissolved to form a homogeneous aqueous solution; after the aqueous solution is naturally cooled to room temperature, slowly add the polypeptide under stirring conditions and continue to stir for a period of time to fully dissolve the polypeptide and uniformly disperse it in the aqueous solution; Step 4, preparation and mixing of the oil phase: Mix hydroxypropyltetrahydropyrantriol, ceramide NP, and an emulsifier in a certain proportion, heat to the molten state and perform emulsification under stirring conditions to form a homogeneous oil-phase emulsion; slowly add the oil-phase emulsion to the aqueous solution prepared in Step 3 under stirring conditions, and continue to stir for a period of time to fully mix the oil phase and the aqueous phase evenly; add nano-active substances to the mixed solution and continue to stir for a period of time to uniformly disperse the nano-active substances in the solution; finally, adjust the pH value of the solution to 5.5 using an appropriate amount of acid or base solution to obtain the final essence.

[0009] Preferably, the high-pressure homogenization pressure in the high-pressure homogenization treatment is 80 MPa, and the liposome suspension needs to be circulated 3 times; the temperature when the aqueous phase prepared in Step 3 and the oil phase prepared in Step 4 are mixed with each other is 35°C, and the stirring speed is set to 200 rpm.

[0010] Preferably, in the first step, the mass ratio of retinol palmitate to vitamin C ethyl ether is 1:2, the volume concentration of ethanol in the hydrogenated lecithin-cholesterol ethanol solution is 70%, the temperature of rotary evaporation is 40°C, the vacuum degree is -0.08 MPa, and the rotary evaporation time is 30 minutes; when performing high-pressure homogenization treatment, the solution temperature is maintained at 25°C.

[0011] Preferably, in the second step, the mass ratio of resveratrol to PLGA is 1:5, the organic solvent is a mixed solvent of dichloromethane and acetone with a volume ratio of 1:1, the surfactant is polyvinyl alcohol (PVA) with a concentration of 2%; the stirring speed of the W / O type primary emulsion is 500 rpm, the stirring speed of the W / O / W type double emulsion is 800 rpm, and the evaporation time of the organic solvent is 4 hours.

[0012] Preferably, in the third step, the addition sequence of glycerol polyether-26, sodium hyaluronate and poria cocos polysaccharide is: first add glycerol polyether-26 and stir for 5 minutes, then add sodium hyaluronate and stir for 10 minutes, and finally add poria cocos polysaccharide and stir for 15 minutes; the addition temperature of the polypeptide is 40°C, and the stirring time is 20 minutes.

[0013] Preferably, in the fourth step, the mass ratio of hydroxypropyltetrahydropyrantriol, ceramide NP to the emulsifier is 8:1:0.5, the emulsifier is triglyceride caprylate / caprate, the heating temperature is 60°C, and the emulsification time is 15 minutes; the dropping speed of the oil-phase emulsion into the water-phase solution is 1 mL / min; The nano-active substance includes liposome-encapsulated vitamin C ethyl ether and resveratrol PLGA microspheres, and the addition sequence is to first add the liposome suspension and stir for 10 minutes, and then add the nano-microsphere suspension and stir for 15 minutes.

[0014] The beneficial effects of the present invention are as follows: 1. Through the synergistic compounding and precise ratio design of polypeptide components, the present invention realizes multi-target coverage of the anti-wrinkle mechanism. Acetyl hexapeptide-8 antagonizes the excessive release of neurotransmitters and effectively inhibits the formation of expression lines caused by muscle contraction; palmitoyl pentapeptide-4, as a signaling molecule, activates the TGF-β pathway and promotes the synthesis of type I collagen in the dermis. Through experimental verification, when the molar ratio of the two reaches 1:1.3, the proliferation rate of fibroblasts can be significantly increased by 42% compared with the single component, forming a combined anti-wrinkle effect of "dynamic wrinkle inhibition + static wrinkle filling".

[0015] 2. Through the innovative application of nanocarrier technology, the present invention has broken through the delivery bottleneck of traditional active ingredients. The liposome encapsulation technology improves the stability of vitamin C ethyl ether by 3.8 times, and the retention rate after 3 months of storage at 4°C is 91.2%. Its 50-nm particle size combined with the biomimetic structure of lecithin enhances the transdermal efficiency by 210% compared with the free ingredient; the PLGA microspheres control the release rate of resveratrol, achieving a cumulative antioxidant release of 89.7% over 72 hours, effectively neutralizing the free radical chain reaction induced by ultraviolet light and prolonging the skin's photoprotective effect time.

[0016] 3. By constructing a multi-dimensional skin repair system, the present invention forms a three-dimensional network of "moisturizing-barrier-immunity". Hydroxypropyltetrahydropyrantriol activates the expression of aquaporin AQP3, increasing the water content of the stratum corneum by 18.5%; ceramide NP has a 92% homology with the skin lipid layer, repairing the barrier function and reducing the trans-epidermal water loss rate (TEWL) by 23%; pachymaran regulates immune homeostasis through the TLR4 receptor, and combined with the 500-fold water retention value of sodium hyaluronate, it forms a water-locking magnet effect in the epidermal layer, maintaining the water content of the stratum corneum above 42% in a dry environment and significantly enhancing the skin's ability to resist external stimuli. Brief Description of the Drawings

[0017] Figure 1 It is a schematic diagram of the preparation process of the anti-wrinkle essence for firming skin of the present invention. Detailed Description of the Invention

[0018] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0019] As Figure 1 shown, the embodiment of the present invention provides an anti-wrinkle essence for firming skin, which is composed of the following raw materials in mass percentages: acetyl hexapeptide-8 accounts for 1.5-2.5%, palmitoyl pentapeptide-4 accounts for 2.0-3.0%, hydroxypropyltetrahydropyrantriol accounts for 3.0%, liposome-encapsulated vitamin C ethyl ether accounts for 2.0%, resveratrol PLGA microspheres account for 0.5%, ceramide NP accounts for 0.8%, pachymaran accounts for 1.2%, glycerol polyether-26 accounts for 5.0%, sodium hyaluronate accounts for 0.3%, p-hydroxyacetophenone and 1,2-hexanediol account for 0.6%, and the balance is deionized water.

[0020] Among them, the molar ratio of acetyl hexapeptide-8 to palmitoyl pentapeptide-4 is 1:1.3.

[0021] Among them, the liposome-encapsulated vitamin C ethyl ether is composed of hydrogenated lecithin and cholesterol in a ratio of 3:1, with a particle size ≤ 50 nm and an encapsulation efficiency ≥ 92%.

[0022] Among them, the resveratrol PLGA microspheres have a particle size of 200 nm and a sustained release time of 72 hours.

[0023] Among them, the preparation method includes the following steps: Step 1, preparation of liposomes: Dissolve retinol palmitate and vitamin C ethyl ether in a hydrogenated lecithin-cholesterol ethanol solution, and completely dissolve retinol palmitate and vitamin C ethyl ether under constant temperature stirring conditions to form a uniform mixed solution; transfer the mixed solution to a rotary evaporator, and perform rotary evaporation to remove ethanol at appropriate temperature and vacuum conditions to concentrate the solution and initially form liposomes; then transfer the product after rotary evaporation to a high-pressure homogenizer for high-pressure homogenization treatment to obtain a liposome suspension with uniform particle size and good dispersibility; Step 2, preparation of nano-microspheres: Dissolve resveratrol in an appropriate amount of organic solvent to form an oil-phase solution, and dissolve PLGA in another organic solvent to form a PLGA solution; slowly drop the resveratrol oil-phase solution into the PLGA solution under stirring conditions to form a water-in-oil W / O primary emulsion; slowly add the primary emulsion into an aqueous solution containing a surfactant to form a water-in-oil-in-water W / O / W multiple emulsion; under stirring conditions, volatilize the organic solvent and solidify PLGA to form nano-microspheres; centrifuge the solidified nano-microsphere suspension to remove the supernatant, wash the nano-microspheres several times with deionized water to remove residual organic solvents and surfactants, and finally disperse the washed nano-microspheres in an appropriate amount of buffer solution for standby; Step 3, preparation of the aqueous phase: Under the condition of a 70 °C constant temperature water bath, sequentially add glycerol polyether-26, sodium hyaluronate, and pachymaran to deionized water, and stir while adding until completely dissolved to form a uniform aqueous solution; after the aqueous solution is naturally cooled to room temperature, slowly add the polypeptide under stirring conditions and continue to stir for a period of time to make the polypeptide fully dissolve and be uniformly dispersed in the aqueous solution; Step 4, preparation and mixing of the oil phase: Mix hydroxypropyltetrahydropyrantriol, ceramide NP, and an emulsifier in a certain proportion, heat to the molten state and perform emulsification under stirring conditions to form a uniform oil-phase emulsion; slowly add the oil-phase emulsion to the aqueous solution prepared in Step 3 under stirring conditions, and continue to stir for a period of time to make the oil phase and the aqueous phase fully mixed and uniform; add nano-active substances to the mixed solution and continue to stir for a period of time to make the nano-active substances uniformly dispersed in the solution; finally, adjust the pH value of the solution to 5.5 with an appropriate amount of acid or alkali solution to obtain the final essence.

[0024] Among them, the high-pressure homogenization pressure in the high-pressure homogenization treatment is 80 MPa, and it needs to be circulated 3 times to obtain the liposome suspension; the temperature when the aqueous phase prepared in Step 3 and the oil phase prepared in Step 4 are mixed with each other is 35 °C, and the stirring speed is set at 200 rpm.

[0025] Among them, in Step 1, the mass ratio of retinol palmitate to vitamin C ethyl ether is 1:2, the volume concentration of ethanol in the hydrogenated lecithin-cholesterol ethanol solution is 70%, the temperature of rotary evaporation is 40 °C, the vacuum degree is -0.08 MPa, and the rotary evaporation time is 30 minutes; the solution temperature is maintained at 25 °C during the high-pressure homogenization treatment.

[0026] Among them, in Step 2, the mass ratio of resveratrol to PLGA is 1:5, the organic solvent is a mixed solvent of dichloromethane and acetone with a volume ratio of 1:1, the surfactant is polyvinyl alcohol (PVA) with a concentration of 2%; the stirring speed of the W / O type primary emulsion is 500 rpm, the stirring speed of the W / O / W type multiple emulsion is 800 rpm, and the organic solvent volatilization time is 4 hours.

[0027] Among them, in Step 3, the addition sequence of glycerol polyether-26, sodium hyaluronate, and poria cocos polysaccharide is: first add glycerol polyether-26 and stir for 5 minutes, then add sodium hyaluronate and stir for 10 minutes, and finally add poria cocos polysaccharide and stir for 15 minutes; the addition temperature of the polypeptide is 40 °C, and the stirring time is 20 minutes.

[0028] Among them, in Step 4, the mass ratio of hydroxypropyltetrahydropyrantriol, ceramide NP, and emulsifier is 8:1:0.5, the emulsifier is triglyceride caprylate / caprate, the heating temperature is 60 °C, and the emulsification time is 15 minutes; the dropping speed when the oil phase emulsion is added to the aqueous phase solution is 1 mL / min; The nano-active substance includes liposome-encapsulated vitamin C ethyl ether and resveratrol PLGA microspheres. The addition sequence is to first add the liposome suspension and stir for 10 minutes, and then add the nano-microsphere suspension and stir for 15 minutes.

[0029] Example 1 Preparation of essence: Liposome preparation; Dissolve 2.0 g of vitamin C ethyl ether in a 70% ethanol solution of hydrogenated lecithin (1.5 g) and cholesterol (0.5 g), and stir at a constant temperature until completely dissolved.

[0030] Transfer to a rotary evaporator and rotary evaporate for 30 minutes at 40 °C and a vacuum degree of -0.08 MPa to remove ethanol.

[0031] Circulate and process 3 times with a high-pressure homogenizer at 80 MPa to obtain a liposome suspension with a particle size of 45 nm and an encapsulation efficiency of 95%.

[0032] Preparation of nano-microspheres Resveratrol (0.1 g) was dissolved in a dichloromethane / acetone (1:1, v / v) mixed solvent and slowly dropped into a PLGA solution (0.5 g of PLGA dissolved in the same mixed solvent).

[0033] A W / O primary emulsion was formed (stirred at 500 rpm), and then dropped into an aqueous phase containing 2% polyvinyl alcohol (PVA) to form a W / O / W multiple emulsion (stirred at 800 rpm).

[0034] After stirring for 4 hours to evaporate the solvent, 200 nm microspheres were obtained after centrifugation and washing, and dispersed in a buffer solution.

[0035] Preparation of the aqueous phase At 70 °C, polyglyceryl ether-26 (5.0 g), sodium hyaluronate (0.3 g), and pachyman polysaccharide (1.2 g) were successively added to deionized water and stirred to dissolve.

[0036] After cooling to room temperature, acetyl hexapeptide-8 (1.5 g) and palmitoyl pentapeptide-4 (2.0 g) were added, and stirred at 40 °C for 20 minutes.

[0037] Preparation and mixing of the oil phase Hydroxypropyltetrahydropyrantriol (3.0 g), ceramide NP (0.8 g), and an emulsifier (0.5 g of triglyceride caprylate / caprate) were mixed and melted and emulsified at 60 °C for 15 minutes.

[0038] The oil phase was dropped into the aqueous phase at 1 mL / min (stirred at 35 °C and 200 rpm), the liposome suspension was added and stirred for 10 minutes, and then the nano-microsphere suspension was added and stirred for 15 minutes.

[0039] The pH was adjusted to 5.5 to obtain a light yellow transparent essence.

[0040] Example 2 The dosages of acetyl hexapeptide-8 (2.5 g) and palmitoyl pentapeptide-4 (3.0 g) were adjusted, and the molar ratio was maintained at 1:1.3. The remaining steps were the same as in Example 1. The obtained essence had a moderate viscosity and no stratification was observed in the stability test (45 °C / 30 days).

[0041] Example 3 The dosage of vitamin C ethyl ether in liposome preparation was increased to 3.0 g, and the homogenization pressure was adjusted to 100 MPa, with the remaining parameters unchanged. The measured liposome particle size was 42 nm and the encapsulation efficiency was 93.5%.

[0042] Performance verification: In vitro transdermal experiment: The transdermal amount of vitamin C ethyl ether in the essence of Example 1 (24 h) was 2.1 times that of the free form.

[0043] Antioxidant test: The DPPH scavenging rate of Example 1 reached 89.2%, and the resveratrol sustained-release microspheres were continuously released within 72 h.

[0044] Clinical evaluation: After 30 volunteers used it for 8 weeks, the skin elasticity value increased by 18.6%, and the area of crow's feet decreased by 15.3%.

[0045] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variation thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article or device.

[0046] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A firming skin anti-wrinkle essence, characterized in that: The firming and anti-wrinkle essence is composed of the following raw materials in mass percentages: acetyl hexapeptide-8 accounts for 1.5 - 2.5%, palmitoyl pentapeptide-4 accounts for 2.0 - 3.0%, propyltetrahydropyranyl glucoside accounts for 3.0%, liposome-encapsulated vitamin C ethyl ether accounts for 2.0%, resveratrol PLGA microspheres account for 0.5%, ceramide NP accounts for 0.8%, pachyman polysaccharide accounts for 1.2%, glycerol polyether-26 accounts for 5.0%, sodium hyaluronate accounts for 0.3%, hydroxyacetophenone and 1,2-hexanediol account for 0.6%, and the balance is deionized water.

2. The anti-wrinkle essence for firming skin according to claim 1, wherein: The molar ratio of acetyl hexapeptide-8 to palmitoyl pentapeptide-4 is 1:1.

3.

3. A firming skin anti-wrinkle essence and its preparation method according to claim 1, characterized in that: The liposome-encapsulated vitamin C ethyl ether is composed of hydrogenated lecithin and cholesterol in a ratio of 3:1, with a particle size ≤ 50 nm and an encapsulation efficiency ≥ 92%.

4. The anti-wrinkle essence for firming skin according to claim 1, characterized in that: The resveratrol PLGA microspheres have a particle size of 200 nm and a sustained release time of 72 hours.

5. A method for preparing the essence according to any one of claims 1-4, characterized in that: The preparation method comprises the following steps: Step 1, preparation of liposomes; dissolve retinyl palmitate and vitamin C ethyl ether in a hydrogenated lecithin-cholesterol ethanol solution, and completely dissolve retinyl palmitate and vitamin C ethyl ether under constant temperature stirring conditions to form a homogeneous mixed solution; transfer the mixed solution to a rotary evaporator, and carry out rotary evaporation to remove ethanol under appropriate temperature and vacuum conditions to concentrate the solution and preliminarily form liposomes; then transfer the product after rotary evaporation to a high-pressure homogenizer for high-pressure homogenization treatment to obtain a liposome suspension with uniform particle size and good dispersibility; Step 2, preparation of nano-microspheres: dissolve resveratrol in an appropriate amount of organic solvent to form an oil-phase solution, and dissolve PLGA in another organic solvent to form a PLGA solution; Under stirring conditions, slowly drop the resveratrol oil-phase solution into the PLGA solution to form a water-in-oil W / O type primary emulsion; slowly add the primary emulsion into an aqueous solution containing a surfactant to form a water-in-oil-in-water W / O / W type multiple emulsion; Under stirring conditions, volatilize the organic solvent and solidify PLGA to form nano-microspheres; perform centrifugal separation on the solidified nano-microsphere suspension to remove the supernatant, wash the nano-microspheres several times with deionized water to remove residual organic solvents and surfactants, and finally disperse the washed nano-microspheres in an appropriate amount of buffer solution for standby; Step 3, preparation of the aqueous phase: under the condition of a 70°C constant temperature water bath, sequentially add glycerol polyether-26, sodium hyaluronate, and pachyman polysaccharide to deionized water, and stir while adding until completely dissolved to form a homogeneous aqueous solution; after the aqueous solution is naturally cooled to room temperature, slowly add polypeptides under stirring conditions and continue to stir for a period of time to fully dissolve the polypeptides and uniformly disperse them in the aqueous solution; Step 4, Preparation and Mixing of Oil Phase: Mix propyltetrahydropyranyl triol, ceramide NP and an emulsifier in a certain proportion, heat to the molten state and emulsify under stirring conditions to form a uniform oil-phase emulsion; slowly add the oil-phase emulsion to the aqueous solution prepared in Step 3 under stirring conditions, and continue stirring for a period of time to fully mix the oil phase and the aqueous phase evenly; add nano-active substances to the mixed solution and continue stirring for a period of time to uniformly disperse the nano-active substances in the solution; finally, adjust the pH value of the solution to 5.5 with an appropriate amount of acid or base solution to obtain the final essence.

6. The preparation method of a firming and anti-wrinkle essence according to claim 5, characterized in that: The high-pressure homogenization pressure in the high-pressure homogenization treatment is 80 MPa, and it needs to be circulated 3 times to obtain a liposome suspension; the temperature when the aqueous phase prepared in Step 3 is mixed with the oil phase prepared in Step 4 is 35 °C, and the stirring speed is set at 200 rpm.

7. The preparation method of a firming skin anti-wrinkle essence according to claim 5, characterized in that: In Step 1, the mass ratio of retinol palmitate to vitamin C ethyl ether is 1:2, the volume concentration of ethanol in the hydrogenated lecithin-cholesterol ethanol solution is 70%, the temperature of rotary evaporation is 40 °C, the vacuum degree is -0.08 MPa, and the rotary evaporation time is 30 minutes; the solution temperature is maintained at 25 °C during high-pressure homogenization treatment.

8. The preparation method of a firming and anti-wrinkle essence according to claim 5, characterized in that: In Step 2, the mass ratio of resveratrol to PLGA is 1:5, the organic solvent is a mixed solvent of dichloromethane and acetone with a volume ratio of 1:1, the surfactant is polyvinyl alcohol with a concentration of 2%; the stirring speed of the W / O primary emulsion is 500 rpm, the stirring speed of the W / O / W double emulsion is 800 rpm, and the organic solvent volatilization time is 4 hours.

9. The preparation method of a firming and anti-wrinkle essence according to claim 5, characterized in that: In Step 3, the addition sequence of glycerol polyether-26, sodium hyaluronate and poria cocos polysaccharide is: first add glycerol polyether-26 and stir for 5 minutes, then add sodium hyaluronate and stir for 10 minutes, and finally add poria cocos polysaccharide and stir for 15 minutes; the addition temperature of the polypeptide is 40 °C, and the stirring time is 20 minutes.

10. The preparation method of a firming and anti-wrinkle essence according to claim 5, characterized in that: In Step 4, the mass ratio of propyltetrahydropyranyl triol, ceramide NP and the emulsifier is 8:1:0.5, the emulsifier is glyceryl caprylate / caprate, the heating temperature is 60 °C, and the emulsification time is 15 minutes; the dropping speed when the oil-phase emulsion is added to the aqueous solution is 1 mL / min; The nano-active substances include liposome-encapsulated vitamin C ethyl ether and resveratrol PLGA microspheres, and the addition sequence is to first add the liposome suspension and stir for 10 minutes, and then add the nano-microsphere suspension and stir for 15 minutes.

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