Mycobacterium tuberculosis fusion protein (EAMMH) and constructing, expressing and purifying method and application thereof

A technology of Mycobacterium tuberculosis and fusion protein, which is applied in chemical instruments and methods, biochemical equipment and methods, peptide preparation methods, etc., can solve problems such as inability to effectively prevent adult pulmonary tuberculosis, and no new anti-tuberculosis vaccines have been developed.
CN104098700AActive Publication Date: 2014-10-15LANZHOU UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LANZHOU UNIVERSITY
Publication Date
2014-10-15

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Abstract

The invention discloses mycobacterium tuberculosis fusion protein EAMMH and a constructing, expressing and purifying method and application thereof. The fusion protein is expressed in a soluble form, and greatly improves EAMM inclusion body form expression weakness. Tuberculosis subunit vaccine (LT69) constructed by combination of the fusion protein and an adjuvant has strong protective immunity, is superior to traditional BCG (Bacillus Calmette-Guerin) vaccine and EAMM+MH combined vaccine; the vaccine as an enhanced vaccine can significantly enhance the BCG initial immune immunity and protection effect of anti tuberculosis, and to a certain extent, reduces the pathological injury of the lung; in addition, the subunit vaccine contains a wide variety of antigens of growth period and latency period of mycobacterium tuberculosis, can induce strong specific cellular immune and humoral immune response aiming at each period of tubercle bacillus antigen, has protective effect on the tubercle bacillus in different metabolic states, is long in protection time, and is expected to become an effective vaccine for clinical tuberculosis prevention.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to mycobacterium tuberculosis fusion protein EAMMH, its construction, expression and purification methods and its application. Background technique

[0002] Mycobacterium tuberculosis is the pathogen of tuberculosis, and it is one of the most adaptable human pathogens to the human environment. It can exist in human macrophages in a latent state for a long time. It is estimated that 2 billion people in the world are latently infected by Mycobacterium tuberculosis, and 10% of this population progresses from latent tuberculosis to active tuberculosis when the immune function is low. Control strategies for tuberculosis rely heavily on long-term treatment with at least three different anti-tuberculosis drugs. As a result, the continuous development of multi-drug resistance poses a huge obstacle to the control of tuberculosis. In countries with the highest rates of tuberculosis, acces...

Claims

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