A kind of culture method of Rhodiola rosea aseptic seedling with well-developed root system

A cultivation method, Rhodiola rosea technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of short seedling age, few roots, weak seedling growth, etc., to simplify the cultivation procedure and reduce the cost of experiments , conducive to the effect of accumulation

Inactive Publication Date: 2016-01-20
JILIN NORMAL UNIV
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Problems solved by technology

[0005] The object of the present invention is to overcome the problems such as weak seedling growth potential, difficult rooting, fewer roots, and short seedling age in the existing Rhodiola alpine tissue culture process, and provide a method for cultivating Rhodiola aseptic seedlings with well-developed root system, To obtain high-quality regenerated plants of Rhodiola rosea

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  • A kind of culture method of Rhodiola rosea aseptic seedling with well-developed root system
  • A kind of culture method of Rhodiola rosea aseptic seedling with well-developed root system
  • A kind of culture method of Rhodiola rosea aseptic seedling with well-developed root system

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Embodiment Construction

[0020] Below in conjunction with implementation case the present invention will be further described:

[0021] (1) Collection of wild seedlings of Rhodiola rosea: In July of each year, wild seedlings of Rhodiola rosea are collected at an altitude of 1700-2500m in Changbai Mountain. When selecting seedlings, young stems should be selected, and the distance from the root should be more than 2cm. In the selection of young stems, care should be taken to keep the tender stems fresh.

[0022] (2) Disinfect the collected stems of Rhodiola rosea wild seedlings: rinse them with running water for 10 minutes, soak them in 75% alcohol for 30 seconds in an ultra-clean workbench, rinse them twice with sterile water, and then rinse them with 10 % NaClO solution for 10 min, rinsed with sterile water 4 times, and set aside.

[0023] (3) Segmentation of the young stems of Rhodiola rosea: Put the sterilized young stems of Rhodiola rosea in a petri dish, put them on the ultra-clean workbench, an...

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Abstract

The invention relates to a method for cultivating Rhodiola rosea aseptic seedlings with well-developed root system, and belongs to the field of modern biological methods. The method comprises the following steps: (1), collecting wild Rhodiola rosea seedlings; (2), disinfecting the collected stems of Rhodiola rosea wild seedlings; (3), collecting young rhodiola rosea alpine stems (4) preparation of culture medium; (5) cultivation of aseptic seedlings of Rhodiola rosea with well-developed root system. The present invention overcomes the disadvantages and deficiencies such as weak seedling growth, difficult rooting, less root number, and short seedling age in the existing Rhodiola rosea tissue culture process, and provides a method for cultivating sterile seedlings of Rhodiola rosea with well-developed root system, In order to obtain high-quality regenerated plants of Rhodiola rosea.

Description

technical field [0001] The invention relates to a method for cultivating Rhodiola rosea aseptic seedlings with well-developed root system, and belongs to the field of modern biological methods. Background technique [0002] Alpine Rhodiola (Rhodiolas activated carbon halinensis A. Bor.), also known as Sakye Rhodiola, is a perennial dicotyledonous herbaceous plant that grows in high-altitude and low-temperature mountainous areas. In my country, it is mostly distributed in the northeast, and it is one of the rare and precious medicinal plants in the Changbai Mountain area. The roots, stems, leaves, flowers, and seeds of Rhodiola alpine can be used as medicine. The active ingredients are salidroside, flavonoids and various trace elements. Rhodiola not only has anti-oxidation, anti-fatigue, anti-hypoxia, Anti-cold, anti-aging, anti-microwave radiation and other similar "adaptogen" effects. Due to the large demand of the pharmaceutical and food industry, wild Rhodiola rosea has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 周晓馥徐洪伟未晓巍
Owner JILIN NORMAL UNIV
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