One-step seedling and efficient in-vitro propagation method with gynura bicolor leaves

A technique for in vitro propagation of G. violaceum, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of long seedling breeding cycle, easy mutation of germplasm, complicated procedures, etc., and achieves good growth and seedlings. The effect of clump growth is robust

Inactive Publication Date: 2014-08-27
ZHAOQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the rapid propagation technology of G. purple-backed so far still uses the traditional tissue culture method. It has to go through multiple stages such as primary culture, subculture, rooting culture and seedling hardening and transplanting. The cycle is long, the germplasm is prone to variation, and the cost is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Medium preparation: MS was used as the basic medium, 0.25 mg of 6-BA, 0.4 mg of NAA, 0.2 mg of IBA, 7 g of carrageenan, and 30 g of sucrose were added per liter, and the pH of the medium was adjusted to 6.0 after mixing ; Dispense each bottle with about 30 mL, put it in an autoclave with sterilization conditions set at 121°C and 105 Kpa for 15 minutes, take out the culture bottle, and cool it for later use;

[0032] (2) Explant treatment and inoculation: According to the conventional inoculation method, the leaves of aseptic seedlings of G. purple-backed seedlings with a leaf diameter of about 0.8-1.5 cm were cut as explants, and the leaves were directly inoculated on the above-mentioned medium (MS+ 0.25 mg / L 6-BA+0.4 mg / L NAA+0.2 mg / L IBA+30 g / L sucrose+7 g / L carrageenan), inoculate 2 explants in each bottle, and aseptically seal the mouth of the bottle;

[0033] (3) Cultivation: Move the inoculated culture bottle into the culture room for culture, the culture cond...

Embodiment 2

[0036] Other operations are the same as in Example 1, except that in step (1), the formula of the medium is (MS+0.25 mg / L 6-BA+0.6 mg / L NAA+0.2 mg / L IBA+30 g / L sucrose + 7 g / L carrageenan); in step ((3), after 15 days of culture, the survival rate of the explants was 100%, the leaves were obviously enlarged, the leaf margins were upturned, and the induction rate of callus cells (or tissues) 100%. After cultivating for 35 days, the leaves of the explants transformed into massive bud clusters, the differentiation rate was 100%, the height of the bud clusters was about 0-7 mm, the growth rate of the bud clusters was faster, and a small amount of complete leaves were formed. Meanwhile, the bud clusters The inside of the clump tissue emits white fluff, and then the fluff grows rapidly into fine roots densely covered with white fluff, and the root tissue is relatively rich. After 45 days of cultivation, the seedling clump begins to form, with bulbs appearing at the base, and the lea...

Embodiment 3

[0038] Other operations are the same as in Example 2, except that in step (1), the formula of the medium is (MS+0.25 mg / L 6-BA+0.8 mg / L NAA+0.2 mg / L IBA+30 g / L sucrose + 7 g / L carrageenan); in step ((3), after 15 days of culture, the survival rate of the explants was 100%, the leaves were significantly enlarged and arched, the leaf margins were upturned, and the callus induction rate was 100%. After 35 days of culture, the leaves of the explants had rapidly transformed into massive bud clusters, the differentiation rate was 100%, the height of the bud clusters was about 2-7 mm, the bud clusters grew vigorously, and a large number of complete leaves were formed. At the same time, the base of the bud clusters had A large number of white fluffy fine roots are formed, and the root system is rich. After 40 days of cultivation, the seedling clusters begin to form, with obvious bulbs at the base, and the leaves are stretched and green. After 65 days of cultivation, the seedling clust...

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PUM

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Abstract

The invention relates to a high-efficiency in vitro propagation method for one-step seedling formation of an endangered plant of the begoniaceae begonia family. The method of the present invention comprises: medium preparation, adopting the adjusted medium, including preparation, packaging and sterilization; adopting the leaves of aseptic seedlings of G. purple-backed aseptic seedlings as explants, directly carrying out aseptic inoculation; The culture bottles of good seeds are moved into the culture room, cultivated under suitable conditions for 70-80 days, and the number of seedlings induced by a single explant is more than 20; without hardening, the regenerated plants are opened and transplanted to suitable conditions for culture. The survival rate of commercial seedlings reaches 100%. According to the method of the present invention, the tissue culture of G. purple-backed seedlings has a short regeneration period, effectively maintains the characteristics of the original species, significantly improves the asexual reproduction coefficient, and is easy to operate, simple in the training procedure, and obviously reduces the cost of raising seedlings. An effective technology for the industrial production of high-quality seedlings of Heliotrope chinensis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a tissue culture method for rapid seedling propagation of rare and endangered plants, in particular to a one-step seedling rapid propagation method for leaf tissue culture of the medicinal and edible homologous plant Begoniaceae Gelonia chinensis. Background technique [0002] Purple-backed geranium ( Begonia fimbristipulata Hance) is a succulent herb belonging to the family Begoniaceae Begonia, a species endemic to China, also known as Danye, Red Heliotrope, and Sanxuezi. It is commonly found in valleys, cliffs, and mosses under sparse forests at an altitude of 200-1 120 m. On wet rocks, it likes warm, humid and cool climates, and is generally distributed in clusters or clusters. The associated plants include mosses, small ferns, and shrubs. Glycyrrhizae can be found in Shenzhen, Dongguan, Zhaoqing and Jiaoling in Guangdong, but Dinghushan in Zhaoqing is the most famous, and its ty...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 邵玲梁廉梁广坚
Owner ZHAOQING UNIV
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