Skin soothing cosmetic composition containing seven kinds of natural compounds

Abstract

The present invention relates to a skin soothing cosmetic composition comprising a complex extract of seven natural substances, which are Phellodendron amurense, Alnus japonica, Foeniculum vulgare, Prunus mume, Lithospermum erythrorhizon, Schisandra chinensis, and Poncirus trifoliata. More specifically, the present invention relates to a cosmetic composition containing seven kinds of natural complex extracts that inhibit the expression of IL-1α and IL-6 mRNA, which cause inflammation induced by UVB exposure, and inhibit the formation of reactive oxygen species, thus exhibiting an antioxidant effect. According to the present invention, the cosmetic composition comprising, as an active ingredient, a complex extract of seven natural substances, which are Phellodendron amurense, Alnus japonica, Foeniculum vulgare, Prunus mume, Lithospermum erythrorhizon, Schisandra chinensis, and Poncirus trifoliata, is characterized by effectively inhibiting the mRNA expression of IL-1α and IL-6, which cause inflammation, and the formation of reactive oxygen species (ROS), and thereby preventing skin inflammation and aging and having a skin-soothing effect.

Description

Skin soothing cosmetic composition containing seven natural complexes

[0001] The present invention relates to a cosmetic composition for skin soothing containing seven kinds of natural complexes, and more particularly, to a natural complex extract having the effect of alleviating skin inflammation by suppressing the expression of mRNA of inflammatory factors (IL-1α, IL-6) that cause inflammation by ultraviolet rays (UVB), and a method for producing the same.

[0002] The skin, the tissue that covers the body's surface, protects against physical stimuli like shock, heat, and sunlight. It also protects against chemical stimuli and bacterial invasion, and helps the body adapt to environmental changes by maintaining a constant body temperature. However, because it is so closely exposed to the external environment, it is also susceptible to sensitization from exposure to UV rays, environmental pollution, and chemicals.

[0003] Cytokines, such as tumor necrosis factor (TNF-α) and interleukins (IL-1α, IL-6), are key components of the inflammatory response and are collectively referred to as inflammatory factors. Inflammation mediated by these inflammatory factors is one of the body's defense mechanisms to minimize the response when cells or tissues are damaged by any cause and to restore the damaged area to its original state. Inflammation is also a primary immune response to identify and eliminate various foreign substances, such as pathogens, that have invaded the body to maintain homeostasis. However, this inflammation causes damage to cells and connective tissues through proteolytic enzymes, and damage to connective tissues reduces skin elasticity, leading to wrinkles and rapid skin aging.

[0004] Meanwhile, reactive oxygen species (ROS) refer to highly reactive radicals containing oxygen, and nitric oxide (NO) is also called reactive nitrogen species (RNS) containing nitrogen atoms (N), but is generally considered a subgroup of reactive oxygen species. Nitric oxide or reactive oxygen species can easily be generated due to environmental pollution, ultraviolet rays, stress, smoking, etc., and if produced in excess, it destroys collagen and elastin, which are fibrous structures in the skin, causing a loss of elasticity, which can lead to aging symptoms such as "wrinkles" and "sagging." In addition, reactive oxygen species are considered to be substances that acidify the skin, cause waste products to accumulate under the skin, and block blood circulation, accelerating skin aging.

[0005] Therefore, suppressing inflammation and reactive oxygen species and soothing irritated skin are crucial elements in preventing skin aging. Consequently, the development of functional materials with anti-inflammatory and antioxidant effects is becoming increasingly important to prevent various skin inflammations and aging.

[0006]

[0007] The purpose of the present invention is to provide a skin soothing cosmetic composition containing seven kinds of natural complexes and a method for producing the same.

[0008] Another aspect of the present invention is to provide a cosmetic composition that effectively suppresses the expression of mRNA of IL-1α and IL-6, which are inflammatory factors that cause inflammation in the skin, and the generation of active oxygen directly related to skin aging.

[0009] Specifically, the purpose of the cosmetic composition of the present invention is to confirm the excellent skin anti-inflammatory and antioxidant effects of an extract obtained by mixing seven kinds of materials, including Phellodendron amurense, Oryzae, Fennel, Prunus mume, Lithospermum officinalis, Schisandra chinensis, and Tangerine, in a specific ratio, and to provide a cosmetic composition comprising the same.

[0010] The technical tasks to be achieved in the embodiments are not limited to those mentioned above, and other technical tasks not mentioned can be considered by a person having ordinary skill in the art from the various embodiments described below.

[0011]

[0012] To achieve these purposes, the present invention provides a cosmetic composition for skin soothing and anti-inflammation, comprising extracts of at least one selected from the group consisting of Phellodendron amurense, Oryza sativa, Fennel, Prunus mume, Lithospermum officinalis, Schisandra chinensis, and Tangerine.

[0013] Specifically, the cosmetic composition may be formed by including a mixed extract obtained by mixing and extracting all of the above Phellodendron amurense, Alder tree, Fennel, Yellow plum, Lithospermum chinensis, Schisandra chinensis, and Tangerine chinensis, and more specifically, may be formed by using a mixed extract obtained by mixing in a ratio of 10 to 20 wt% of Phellodendron amurense, 21 to 28 wt% of Alder tree, 2 to 7 wt% of Fennel, 2 to 7 wt% of Yellow plum, 10 to 14 wt% of Lithospermum chinensis, 29 to 35 wt% of Schisandra chinensis, and 5 to 12 wt% of Tangerine chinensis. In one embodiment of the present invention, the cosmetic composition was manufactured by including 15 wt% of Phellodendron amurense, 24 wt% of Alder tree, 4 wt% of Fennel, 4 wt% of Yellow plum, 12 wt% of Lithospermum chinensis, 32 wt% of Schisandra chinensis, and 9 wt% of Tangerine chinensis, but is not limited thereto.

[0014] In the present invention, the phellodendron amurense is the dried bark of Phellodendron amurense, a member of the Zanthoxylum family. Phellodendron amurense is a deciduous broad-leaved tree that grows deep in the mountains, reaching a height of approximately 10 m. Its bark is covered with cork and its inner bark is yellow. The leaves are lanceolate and obovate, and the leaf margins have fine teeth and soft hairs. The berberine component of Phellodendron amurense exhibits hypotensive effects, antibacterial effects against pathogenic microorganisms, and anti-inflammatory effects.

[0015] The alder tree described herein can reach a height of 20 m, has hairy young branches, and its leaves grow alternately and are oblong. Alder trees grow in low-lying wetlands and are cultivated throughout Korea, as well as in Japan, Manchuria, and Siberia. In oriental medicine, the bark or buds are used as a medicinal herb for fever reduction, hemostasis, and digestive inflammation.

[0016] The fennel plant in the present invention is an annual plant of the Apiaceae family, native to the Mediterranean coast. It grows 1.5 to 2 meters tall, has a straight, hollow stem, and its leaves are long, sheath-shaped, pointed, and finely divided into three to four feather-like lobes. It is used worldwide as a food or spice, and its diverse pharmacological properties have led to its use in Oriental medicine. The phenolic compounds in fennel are known to exhibit excellent analgesic and anti-inflammatory effects.

[0017] The plum in the present invention is the fruit of the plum tree, native to China. It grows to a height of 5-10 m, has alternate, ovate leaves, sharply serrated edges, and hairs on both sides. Its light red flowers bloom before the leaves, a characteristic feature. Plums are rich in organic acids, promoting carbohydrate metabolism and relieving fatigue. They also have excellent detoxification properties, helping to treat conditions like stomach upset and food poisoning.

[0018] The lily of the valley plant of the present invention is a perennial herb belonging to the family Boraginaceae. Its stem is straight, and its roots branch out in a ginseng-like pattern. The purple pigment in the root forms around the outer skin, resulting in a strong purple color. The presence of purple pigment in the root is a distinguishing characteristic. The root has been used as a dye and medicinal ingredient since ancient times, and is known to be particularly effective in treating skin inflammation, including dermatitis, freckles, and rashes.

[0019] The five flavors mentioned in the present invention are named after the five flavors they possess, a harmonious balance of sour, sweet, spicy, bitter, and salty. They grow in Korea, Japan, China, and other countries. Omija contains ingredients such as schizandrin and gomisin, which are known to have health benefits such as strengthening the heart, lowering blood pressure, and boosting immunity.

[0020] In the present invention, the tangerine is the fruit of the tangerine tree. The tangerine tree is a deciduous broadleaf shrub belonging to the Rutaceae family, growing approximately 3 m tall and bearing white flowers in May. The tangerine fruit is round, ripens to a yellow color, and has a pleasant fragrance, but is inedible. The tangerine contains essential oils such as limonene, naringin, and ponsyrin. In oriental medicine, it has been used to treat bronchial diseases.

[0021] In the present invention, the "extract" refers to a solvent into which an effective ingredient contained in an extracted raw material is transferred by contacting the solvent and the extracted raw material under specific conditions, and can include any substance obtained by separating a component contained in a raw material from a natural material, regardless of the extraction method or type of component. For example, it can include all substances obtained by extracting a component soluble in a solvent from a natural material using water or an organic solvent, and substances obtained by extracting only a specific component of a natural material, such as a specific component such as an oil, and in the present invention, it can particularly be an essential oil, but is not limited thereto.

[0022] In addition, the extract may include all forms of extracts that can be formed using the extract, such as extracts derived from the above-mentioned natural products, dilutions or concentrates of the extracts, dried products obtained by drying the extracts, preparations or purified products of the extracts, or mixtures thereof. Furthermore, the extracts may be extracted from natural, hybrid, or mutant plants, and may also be extracted from plant tissue cultures.

[0023] The extract of the present invention can be extracted using methods commonly used in the art. Non-limiting examples of the extraction methods include cold immersion extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, and extrusion extraction, and these may be performed alone or in combination of two or more methods.

[0024] The type of extraction solvent used in the present invention is not particularly limited, and any solvent known in the art can be used as needed. Non-limiting examples of the extraction solvent include water; lower alcohols having C1 to C4, such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols, such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents, such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; or mixtures thereof. Specifically, water, lower alcohols, 1,3-butylene glycol, and ethyl acetate can be used alone or in combination of two or more. In addition, the extract extracted as described above can be used as is after filtering, for example, filtering out particles using nylon or the like, or filtering using a freeze filtration method, etc., to remove floating solid particles, or can be used after drying using freeze drying, hot air drying, spray drying, etc.

[0025] In one embodiment of the present invention, 20 g of each of the seven kinds of natural products were separately immersed in 200 g of 70% ethanol, extracted at 60°C for 4 hours, filtered through a filter paper (Whatman No. 2), concentrated, and dissolved in a solvent (butylene glycol 30%, purified water 70%) to make the obtained concentrate 1.0% (w / w), and then filtered through a filter paper (Durapore 0.22 μm, PVDF) to obtain a natural product extract for each of the seven kinds of natural products of the present invention, but the present invention is not limited to this method.

[0026] The cosmetic composition of the present invention is characterized in that it effectively suppresses the mRNA expression of inflammatory factors IL-1α and IL-6 and the production of reactive oxygen species (ROS), thereby preventing skin inflammation, preventing aging, and having a skin soothing effect. Each of these effects was confirmed through specific experimental examples (Experimental Examples 3 to 5).

[0027] In the present invention, the cosmetic composition may be formulated as one or more selected from the group consisting of shampoo, skin, toner, emollient lotion, nourishing lotion, moisturizing cream, nourishing cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, mist, spray, and powder. Specifically, the cosmetic composition may be formulated in the form of a shampoo, but is not limited thereto.

[0028] The above cosmetic composition can be formulated by a conventional method. For the formulation of external skin preparations, reference may be made to the contents disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, and for the formulation of cosmetic compositions, reference may be made to the contents disclosed in International cosmetic ingredient dictionary, 6th ed. (The Cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995).

[0029] Specifically, the cosmetic composition may be prepared in a general emulsified formulation or a solubilized formulation. For example, the cosmetic composition may be formulated as a toner such as a flexible toner or a nourishing toner; an emulsion such as a facial lotion or a body lotion; a cream such as a nourishing cream, a moisturizing cream, or an eye cream; an essence; a cosmetic ointment; a spray; a gel; a pack; a sunscreen; a makeup base; a foundation such as a liquid type, a solid type, or a spray type; a powder; a makeup remover such as a cleansing cream, a cleansing lotion, or a cleansing oil; or a cleanser such as a cleansing foam, soap, or a body wash, but is not limited thereto. In addition, the cosmetic composition may be formulated as an ointment, a patch, a gel, a cream, or a spray, but is not limited thereto.

[0030] In addition to the essential ingredients, other ingredients may be appropriately blended into each formulation of the above cosmetic composition, within a range that does not impede the purpose of the present invention, depending on the type of formulation or intended use.

[0031] The above cosmetic composition may typically include an acceptable carrier, and may appropriately mix, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., but is not limited thereto.

[0032] The above acceptable carriers may vary depending on the formulation. For example, when formulated as an ointment, paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or mixtures thereof may be used as carrier components.

[0033] When the above cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane, butane or dimethyl ether may be further included.

[0034] When the above cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizer, or an emulsifier can be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-butylglycol oil can be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil, and sesame oil, glycerol fatty acid ester, polyethylene glycol, or fatty acid ester of sorbitan can be used.

[0035] When the above cosmetic composition is formulated as a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used as a carrier component.

[0036] When the above cosmetic composition is formulated as soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. can be used as carrier components.

[0037] The above cosmetic composition may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestering agents, chelating agents, preservatives, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, and any other ingredients commonly used in cosmetics, depending on the quality or function of the final product.

[0038] However, the above-mentioned auxiliary agent and its mixing ratio can be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

[0039] According to the present invention, a cosmetic composition comprising a composite extract of seven natural products, including Phellodendron amurense, Oryzae, Fennel, Japanese apricot, Lithospermum chinensis, Schisandra chinensis, and Tangerine, as an active ingredient is characterized in that it effectively suppresses the mRNA expression of IL-1α and IL-6 that cause inflammation and the production of reactive oxygen species (ROS), thereby preventing skin inflammation and aging and having a skin soothing effect.

[0040] In addition, the cosmetic composition of the present invention is useful in various formulations such as shampoo, skin, toner suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, and spray.

[0041] In addition, the present invention is characterized in that it is harmless to the human body and can be easily applied to products by including a natural product-derived extract.

[0042] The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the detailed description of the present invention or the composition of the invention described in the claims.

[0043] The accompanying drawings, which are included as part of the detailed description to aid understanding of the embodiments, provide various embodiments and, together with the detailed description, describe technical features of the various embodiments.

[0044] Figure 1 shows the IL-1α expression inhibitory effect of the complex extract according to Examples 2 to 5 of the present invention.

[0045] Figure 2 shows the IL-6 expression inhibitory effect of the complex extract according to Examples 2 to 5 of the present invention.

[0046] Figure 3 shows the ROS production inhibition effect of the composite extract according to Examples 2 to 5 of the present invention.

[0047] The following embodiments combine components and features of the embodiments in a predetermined form. Each component or feature may be considered optional unless explicitly stated otherwise. Each component or feature may be implemented without being combined with other components or features. Furthermore, various embodiments may be formed by combining some components and / or features. The order of operations described in various embodiments may be changed. Some components or features of one embodiment may be included in another embodiment or may be replaced with corresponding components or features of another embodiment.

[0048]

[0049] Example 1. Preparation of natural product extracts

[0050] The cosmetic composition of the present invention is manufactured using seven kinds of natural products, and extracts of each of these products were manufactured.

[0051] Specifically, seven kinds of natural products, Schisandra chinensis, Oryzae, Phellodendron amurense, Lithospermum Parkii, Tangerine, Fennel, and Yellow Plum, were each prepared in a dried state, and 20 g of each of these natural products were separately soaked in 200 g of 70% ethanol, extracted at 60℃ for 4 hours, filtered through a filter paper (Whatman No. 2), and concentrated. The obtained concentrate was dissolved in a solvent (butylene glycol 30%, purified water 70%) to 1.0% (w / w), and then filtered through a filter paper (Durapore 0.22 μm, PVDF) to obtain natural product extracts for each of the seven kinds of natural products of the present invention.

[0052]

[0053] Experimental Example 1. Confirmation of the skin soothing effect through inhibition of IL-1α activity of individual materials.

[0054] It was evaluated whether each of the above natural product extracts exhibited an effect of suppressing skin inflammation by inhibiting the expression of interleukin-1α (IL-1α).

[0055] Specifically, to confirm the true efficacy, an IL-1α gene expression experiment was performed. HaCaT (immortalized keratinocyte) cells were seeded in 60 mm dishes and cultured for 24 hours. The medium was replaced with serum-free medium, and 50 mJ / cm2 of Ultraviolet B (UVB), which causes inflammatory reactions such as sunburn, erythema, and edema in HaCaT, was irradiated. The samples were treated at various concentrations and cultured for 4 hours in a 37°C, CO25% incubator. After culture, the cells were harvested with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. The extracted total RNA was quantified, and cDNA was synthesized from 1 μg of RNA using a cDNA synthesis kit on a ProFlex PCR system (Applied Biosystems). To confirm gene expression, a 7500 fast real-time PCR system (Applied Biosystems) was used, and the results are shown in Table 1 below.

[0056] As a result of the cytotoxicity evaluation, it was confirmed that there was no toxicity when treated at a concentration of 1% for Schisandra chinensis, Oryzae, Phellodendron amurense, and Zizyma chinensis, and at a concentration of 0.5% for Tangerine, Fennel, and Yellow Plum, and the experiment was conducted accordingly.

[0057] Sample UVB concentration (%) IL-1α mRNA expression inhibition rate (%) Control--93.6 UVB 50mJ-0.0 Quercetin (μM) 50mJ 2534.2 Schisandra chinensis 50mJ 1.082.8 Alder tree 50mJ 1.062.6 Phellodendron amurense 50mJ 1.043.4 Ligusticum chinensis 50mJ 1.043.8 Tangerine chinensis 50mJ 0.537.8 Fennel 50mJ 0.524.6 Yellow plum 50mJ 0.528.2

[0058] As a result of the IL-1α expression inhibition evaluation, as shown in Table 1, Schisandra chinensis showed the highest IL-1α expression inhibition activity at 82.8%. All seven materials were confirmed to be effective in inhibiting IL-1α expression, and in particular, Schisandra chinensis, Oryzae, Phellodendron amurense, Lithospermum chinensis, and Tangerine chinensis showed a superior inhibition rate than the positive control, quercetin.

[0059] Experimental Example 2. Confirmation of the skin soothing effect through the inhibition of IL-6 activity of individual materials.

[0060] We evaluated whether each of the above natural product extracts exhibited an effect of suppressing skin inflammation by inhibiting the expression of interleukin-6 (IL-6).

[0061] Specifically, an IL-6 gene expression experiment was performed to confirm the true efficacy. HaCaT (immortalized keratinocyte) cells were seeded on 60 mm dishes and cultured for 24 hours, then replaced with serum-free medium, and irradiated with Ultraviolet B (UVB) at 50 mJ / cm2, which causes inflammatory reactions such as sunburn, erythema, and edema in HaCaT, and the samples were treated at various concentrations and cultured for 4 hours in a 37℃, CO25% incubator. After culture, the cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. The extracted total RNA was quantified, and then cDNA was synthesized from 1 μg of RNA using a cDNA synthesis kit using a ProFlex PCR system (Applied Biosystems). To confirm gene expression, a 7500 fast real-time PCR system (Applied Biosystems) was used, and the results are shown in Table 2 below.

[0062] As a result of the cytotoxicity evaluation, it was confirmed that there was no toxicity when treated at a concentration of 1% for Schisandra chinensis, Oryzae, Phellodendron amurense, and Lithospermum chinensis, and at a concentration of 0.5% for Tangerine, Fennel, and Yellow Plum, and the experiment was conducted accordingly.

[0063] Sample UVB concentration (%) IL-6 mRNA expression inhibition rate Control--3.2 UVB 50mJ-100.0 Quercetin (μM) 50mJ 2544.4 Schisandra chinensis 50mJ 1.082.0 Alder tree 50mJ 1.077.4 Phellodendron amurense 50mJ 1.046.5 Ligusticum chinensis 50mJ 1.042.1 Tangerine chinensis 50mJ 0.531.6 Fennel 50mJ 0.539.0 Yellow plum 50mJ 0.540.1

[0064] As a result of the IL-6 expression inhibition evaluation, as shown in Table 2, Omija showed the best activity at 82.0%. All seven materials were confirmed to be effective in inhibiting IL-6 expression, and in particular, Omija, Oryzae, and Phellodendron amurense showed a superior interleukin-6 expression inhibition rate than the positive control, quercetin.

[0065] Examples 2 to 5. Preparation of seven types of composite extracts

[0066] The composite extracts of Examples 2 to 5 of the present invention were prepared by mixing in the weight ratios shown in Table 3 below.

[0067] The composite extract of Example 2 was prepared by mixing seven materials in equal proportions, and for Examples 3 to 5, the extracts were mixed and extracted in the weight ratios shown in Table 3 below, taking into account the results of Experimental Examples 1 and 2, so as to exhibit the best anti-inflammatory effect.

[0068] 50 g of 7 kinds of materials mixed in the ratios of Examples 2 to 5 were immersed in 500 g of 70% ethanol, extracted at 60°C for 4 hours, filtered through filter paper (Whatman No. 2), and concentrated. The obtained concentrate was dissolved in a solvent (butylene glycol 30%, purified water 70%) to a concentration of 1.0% (w / w), filtered through filter paper (Durapore 0.22 μm, PVDF), and then used.

[0069] Raw material Example 2 Example 3 Example 4 Example 5 Schisandra chinensis 14.3% 32.0% 28.5% 40.0% Alder 14.3% 24.0% 28.5% 20.0% Phellodendron amurense 14.3% 15.0% 14.3% 10.0% Lithospermum 14.3% 12.0% 14.3% 9.0% Tangerine 14.3% 9.0% 4.8% 7.0% Fennel 14.3% 4.0% 4.8% 7.0% Yellow plum 14.3% 4.0% 4.8% 7.0%

[0070] Experimental Example 3. Confirmation of the skin soothing effect of the complex extract through inhibition of IL-1α activity.

[0071] In order to evaluate whether the composite extracts of Examples 2 to 5 inhibit the expression of IL-1α and are effective in suppressing skin inflammation, an experiment was conducted using the same method as Experimental Example 1, and the results are shown in Table 4 below.

[0072] Sample UVB Concentration (%) IL-1α Inhibition Rate (%) Control--93.6 UVB 50mJ-0.0 Quercetin (μM) 50mJ 2534.2 Example 250mJ 3.0 11.6 Example 350mJ 3.0 38.2 Example 450mJ 3.0 24.7 Example 550mJ 3.0 27.3

[0073] As shown in Table 4, Example 3 showed an inhibition rate of 38.2%, which was close to the positive control. On the other hand, Example 2 showed a low inhibition rate of 11.6%. In addition, it was confirmed that the effect was significantly superior in the ratio of Example 3 compared to the results of Example 5, which contained the largest amount of Schisandra chinensis, which was the most effective when used alone, and Example 4, which contained a larger amount of Oryzae.

[0074] Experimental Example 4. Confirmation of the skin soothing effect of the complex extract through inhibition of IL-6 activity.

[0075] In order to evaluate whether the complex extracts of Examples 2 to 5 are effective in suppressing skin inflammation by inhibiting the expression of IL-6, an experiment was conducted using the same method as Experimental Example 2, and the results are shown in Table 5 below.

[0076] Sample UVB Concentration (%) IL-6 Inhibition Rate Control--95.8 UVB 50mJ-0.0 Quercetin (μM) 50mJ 2546.5 Example 250mJ 3.0- Example 350mJ 3.0 22.9 Example 450mJ 3.0 10.8 Example 550mJ 3.0 12.1

[0077] As shown in Table 5, in the case of Example 3, an inhibition rate of 22.9% was confirmed, whereas in the case of Example 2, the expression rate was 113.2%, showing that the expression of IL-6 was rather promoted. In addition, the results of Examples 4 and 5 showed lower effects than Example 3, and considering that this is the same as Experimental Example 3, the contents of Schisandra chinensis and Oryzae, which showed the best effect as a single raw material, increased, it can be seen that this is the result of the synergistic effect according to the mixing ratio of Example 3.

[0078] Experimental Example 5. Confirmation of antioxidant effect through inhibition of ROS production.

[0079] To evaluate whether the composite extracts of Examples 2 to 5 exhibit antioxidant effects by inhibiting the production of reactive oxygen species (ROS), experiments were conducted using the following method.

[0080] To confirm the antioxidant efficacy, a Reactive Oxygen Species (ROS) assay was performed. HaCaT (immortalized keratinocyte) cells were seeded in 96-well plates and cultured for 24 hours. After replacing with serum-free medium, samples were treated at various concentrations and cultured for 24 hours in a 37°C, CO25% incubator. After culture, the cells were washed with HBSS, treated with H2DcFDA solution, and incubated in an incubator for 30 minutes in a light-shielded environment. After washing with HBSS, 100 mM H2O2 solution was treated, incubated in an incubator for 10 minutes, and the fluorescence value was measured to determine the amount of ROS produced. The results are shown in Table 6 below.

[0081] Sample Concentration (%) ROS Production Inhibition Rate (%) Control-0.0 EGCG (ppm) 1.0 2 6.48 Example 20.75 4.56 Example 30.75 19.23 Example 40.75 7.50 Example 50.75 9.87

[0082] As shown in Table 6, Example 3 showed an inhibition rate of 19.23%, whereas Example 2 showed a low inhibition rate of 4.56%. In addition, Example 4 showed 7.50%, and Example 5 showed 9.87%, which were higher than Example 2, but only about half the effect compared to Example 3. When these experimental results are combined, it can be confirmed that the seven types of complex extracts of the present invention and the cosmetic composition containing the same can effectively suppress the expression of mRNA of inflammatory factors IL-1α and IL-6 and the generation of active oxygen directly related to skin aging.

[0083] The various embodiments described above may be embodied in other specific forms without departing from the technical idea and essential characteristics thereof. Therefore, the above detailed description should not be construed as limiting in all respects but rather as illustrative. The scope of the various embodiments should be determined by a reasonable interpretation of the appended claims, and all changes within the equivalent scope of the various embodiments are included within the scope of the various embodiments. Furthermore, claims that are not explicitly cited in the claims may be combined to form an embodiment or incorporated into a new claim through a post-application amendment.

Claims

1. Containing extracts of one or more selected from the group consisting of Phellodendron amurense, Oryzae, Fennel, Prunus mume, Licorice, Schisandra chinensis and Tangerine. Cosmetic composition for soothing skin and preventing inflammation.

2. In paragraph 1, The above extract is obtained by mixing and extracting Phellodendron amurense, Oryzae, Fennel, Prunus mume, Licorice, Schisandra chinensis and Tangerine. Cosmetic composition for soothing skin and preventing inflammation.

3. In paragraph 2, The above extract is a mixed extract mixed in a ratio of 10 to 20 wt% of Phellodendron amurense, 21 to 28 wt% of Oryza sativa, 2 to 7 wt% of Anise, 2 to 7 wt% of Yellow Plum, 10 to 14 wt% of Lithospermum officinalis, 29 to 35 wt% of Schisandra chinensis, and 5 to 12 wt% of Tangerine. Cosmetic composition for soothing skin and preventing inflammation.

4. In the first paragraph, the cosmetic composition is characterized by inhibiting the expression of IL-1α and IL-6. Cosmetic composition for soothing skin and preventing inflammation.

5. In the first paragraph, the cosmetic composition is characterized in that it additionally has an antioxidant effect by suppressing the production of reactive oxygen species (ROS). Cosmetic composition for soothing skin and preventing inflammation.

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