The invention discloses a biological Chinese medicine film and a preparation method thereof. The biological Chinese medicine film comprises the main medicine components of inactivated brewers' yeast, fructus forsythia, wild chrysanthemum flower and pepper, and also contains additives such as sodium carboxymethylcellulose as a film forming material, glycerol as a polasticizer and peppermint oil as an aromatic. The preparation method of the biological Chinese medicine film comprises the following steps of: firstly, preparing a film forming material size, then adding the inactivated brewers' yeast, a Chinese medicine concentrated solution and the additives, uniformly stirring; and then defoaming, coating the film, drying, removing the film, and packaging. Compared with the prior art, the biological Chinese medicine film provided by the invention plays a role of protecting the surface of a wound, also plays roles in resisting bacteria and virus infection, diminishing inflammation and relieving pain, is capable of improving mouth mucosa nutrition metabolism, protecting various cells from being damaged and enhancing immunity of the organism, has the characteristics of simple formulation, good medicine compatibility, small volume, light weight, convenience for package, storage, transportation, carrying and application and the like, and is mainly applied to various ulcer venereal diseases.