Semiaquilegia root extract, preparation method and application thereof
A technology of extract and Tiankui, which is applied to the preparation of medicines for preventing and treating cataracts.
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Embodiment 1
[0030] Embodiment 1 of the present invention: preparation of geranium extract sample
[0031] Take the fresh or dried geranium, chop it up, and extract with water, methanol, ethanol, acetone; or a mixed solvent of water, methanol, ethanol, or acetone in any proportion as the extraction solvent. The extraction method is decoction, cold immersion, reflux, ultrasonic extraction, supercritical fluid extraction or microwave extraction, and the obtained extract is evaporated under reduced pressure, vacuum freeze-dried or spray-dried to remove the solvent to obtain the geranium extract. The specific raw materials and their weight, the extraction solvent used, the extraction method adopted, and the solvent removal method are shown in Table 1.
[0032] The preparation method of table 1 geranium extract sample
[0033]
[0034]
Embodiment 2
[0035]Example 2 of the present invention: identification of characteristic active ingredients in geranium extract
[0036] 1. TLC identification of tangelofolidine
[0037] Weigh about 1 g of the samples TK1-TK13 in Example 1 and place them in a 20 mL Erlenmeyer flask with a stopper, add 20.00 mL of methanol, ultrasonically extract for 30 min, take out, filter, and concentrate the filtrate in a water bath to 5 mL, respectively as the test samples TK1-TK6 solution. In addition, take the reference substance of tangconidine, add methanol to make a solution with a concentration of 1mg / mL, and use it as the reference substance solution. According to "Chinese Pharmacopoeia" 2005 edition an appendix VIB thin-layer chromatography, draw need testing solution 10 μ L respectively, reference substance solution 2 μ L, point on the same silica gel GF254 thin-layer plate, with n-butanol-glacial acetic acid-water (7 μ L) : 1: 2) solution is the developing agent, second development, take out...
Embodiment 3
[0041] Example 3 of the present invention: further purification of geranium extract samples
[0042] 1. Take an appropriate amount of geranium extract (TK5), disperse and dissolve it with an appropriate amount of water, repeatedly extract it several times with petroleum ether, then repeatedly extract the water layer with ethyl acetate and n-butanol, and recover the solvent respectively to obtain the ethyl acetate part ( TK5A) and n-butanol fraction (TK5B) and water fraction (TK5C).
[0043] 2. Take an appropriate amount of geranium extract (TK1), dissolve it with a small amount of methanol, put it on the D-101 macroporous resin column that has been pretreated, first elute with distilled water, then elute with 20% ethanol, and then use Elute with 50% ethanol, collect the 50% ethanol eluate, and recover the solvent to obtain a further purified sample (TK1A).
[0044] 3. Take an appropriate amount of geranium extract (TK1), dissolve it with a small amount of methanol and put it ...
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