Nucleotide to 0-antigen specificity of escherichia coli 0154 type

Abstract

The invention provides a nucleotide specific to O-antigens of Escherichia coli O154, a full sequence of gene cluster in Escherichia coli O154, controlling O-antigen synthesis, such as the separated nucleotide shown in SEQ IN No.1, with a overall length of 13635 basic groups; or a nucleotide in SEQ IN No.1 having one or many inserted, deleted or substituted basic groups and simultaneously maintaining functions of the separated nucleotide; also including an oligonucleotide coming from glycosyltransferase gene and oligosaccharide unit-processing gene in the O-antigen gene cluster of Escherichia coli O154; by PCR, the invention verifies that oligonucleotide has specificity to all the O-antigens of Escherichia coli O154; the invention also discloses a method of using the oligonucleotide to detect and identify Escherichia coli O154 in the human body and environment.

Description

technical field

[0001] The present invention relates to the complete nucleotide sequence of the gene cluster controlling O-antigen synthesis in Escherichia coli O154 type (Escherichia coli O154), in particular to oligonucleotides in the gene cluster controlling O-antigen synthesis in Escherichia coli O154 type , these O-antigen-specific oligonucleotides can be used to quickly and accurately detect Escherichia coli O154 in the human body and the environment and identify the O-antigens in these pathogenic bacteria. Background technique

[0002] O-antigen is the O-specific polysaccharide component of Gram-negative bacterial lipopolysaccharide, which consists of many repeating oligosaccharide units. The synthesis process of O-antigen has been studied clearly: first, the nucleoside diphosphate monosaccharide is transferred to a lipid molecule fixed on the inner membrane of the cell by glycosyltransferase, and then the oligosaccharide unit is synthesized inside the inner membrane,...

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