Method for rapidly and quantitatively analyzing type B paratyphoid salmonella in cucumbers on basis of prediction models

A technology of paratyphoid B and Salmonella, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the problems of bacterial contamination and neglect, and achieve easy operation and save food Effects of cultivation and detection of source pathogenic bacteria

Inactive Publication Date: 2018-01-09
TIANJIN INSTITUE OF QUALITY STANDARD & TESTING OF AGRICULTUAL PRODS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Due to the large difference between my country's food consumption patterns and European and American countries, most vegetables are eaten after heat processing, so the problem of pathogenic bacteria pollution has not been taken seriously.
[0004] At present, there are few reports on the outbreak of pathogenic bacteria on fresh vegetables in my country. In order to control the occurrence of Salmonella contamination incidents, a large number of scholars have studied the survival and growth of different Salmonella serotypes on various vegetables, and developed a variety of Salmonella. Prevention and control measures

Method used

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  • Method for rapidly and quantitatively analyzing type B paratyphoid salmonella in cucumbers on basis of prediction models
  • Method for rapidly and quantitatively analyzing type B paratyphoid salmonella in cucumbers on basis of prediction models
  • Method for rapidly and quantitatively analyzing type B paratyphoid salmonella in cucumbers on basis of prediction models

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Fresh cucumbers are peeled with a sterilized knife in a sterile room and cut into 1cm 3 For the left and right small dings, spread them evenly in a sterile ultra-clean bench, sterilize them under ultraviolet light for 30 minutes, then turn off the ultraviolet light and ventilate them in the ultra-clean bench for 30 minutes to remove ultraviolet residues. Aseptically pack them into 20g each in In aseptic homogeneous bags, 110 bags are divided into 110 bags, and placed in a 4°C refrigerator for later use;

[0050] (2) Randomly select 2 parts of the above-mentioned aliquoted samples, add 180mL of buffered peptone water respectively, homogenize with a slap-type homogenizer for 1min, take 0.2mL and spread it on the surface of a bismuth sulfite (BS) agar plate, and incubate at 36°C for 48h. Carry out the background detection of cucumber, the background requires no growth of Salmonella;

[0051] (3) Use a sterile pipette to draw 1mL with a concentration of 2×10 4 Inocula...

Embodiment 2

[0056] (1) Use: for market supervision, shelf life prediction of samples positive for Salmonella paratyphi b in cucumber samples in farmers' markets;

[0057] (2) Prediction method: Through market random inspection, it is detected that Salmonella paratyphi b in a cucumber sample is positive, record the initial amount of bacteria and the ambient temperature when the sample is sold at the farmer's market stall at that time, and use the prediction model established to obtain the temperature condition Under the maximum specific growth rate, the safe shelf life time of the cucumber sample can be predicted by the maximum specific growth rate, initial bacterial load, and ambient temperature, and the predicted value when stored at 35°C is obtained by using the established growth kinetic model. The Salmonella growth values ​​of the actual samples in the fresh-cut cucumber storage experiment at 35°C were compared, and the results are shown in Table 2.

[0058] Table 2

[0059]

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Abstract

The invention discloses a method for rapidly and quantitatively analyzing Salmonella paratyphi B in cucumber based on a predictive model. The method is based on traditional microbiology cultivation, combined with mathematics, statistics and other disciplines to simulate Salmonella paratyphi B in cucumber at 10, l5, 20, 25, 30 and 35 °C growth curves under different temperature conditions, construct a first-level and second-level prediction model, apply this model to carry out risk prediction on the initial bacterial load of commercially available cucumber samples at any ambient temperature, and Ensure the safety and quality of cucumbers in a series of links such as production, processing, transportation, sales, and storage, and provide technical support for cucumber safety supervision.

Description

technical field [0001] The invention belongs to the technical field of food microorganism identification and application, and specifically relates to a method for rapid and quantitative analysis of Salmonella paratyphi B in cucumber based on a prediction model. The method is based on traditional microbiology cultivation and combines mathematics, statistics and other disciplines to simulate cucumber The growth curves of Salmonella paratyphi b in China under different temperature conditions of 10, 15, 20, 25, 30 and 35°C were used to construct the first-level and second-level prediction models, and the model can be used to analyze the commercially available cucumber samples at any ambient temperature. Risk prediction is carried out on the initial amount of bacteria carried in order to ensure the safety and quality of cucumbers in a series of links such as production, processing, transportation, sales, and storage, and provide technical support for the safety supervision of cucumb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12Q1/06C12R1/42
Inventor 赵新兰青阔刘娜陈锐王成沈晓玲兰璞朱珠王永
Owner TIANJIN INSTITUE OF QUALITY STANDARD & TESTING OF AGRICULTUAL PRODS
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