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Kit for separating and identifying salmonella, and preparation and application thereof

A Salmonella and kit technology, which is applied in biochemical equipment and methods, microorganism-based methods, and microbial determination/inspection, etc., can solve the problems of high missed detection rate, complicated identification steps, and high cost.

Inactive Publication Date: 2012-11-28
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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Problems solved by technology

It mainly solves the technical problems of high missed detection rate, complicated identification steps and high cost, and unintuitive screening results existing in the existing salmonella isolation methods

Method used

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  • Kit for separating and identifying salmonella, and preparation and application thereof

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Embodiment Construction

[0050] Firstly, kits are prepared according to the content of the technical plan, and users of different laboratories (clinical laboratories and public health laboratories according to functional attributes) choose to use different enrichment solutions or selective plate combinations for different test materials according to the testing requirements Isolate, and finally use simple biochemical and specific enzyme-substrate reaction tests to differentially diagnose typhoid, paratyphoid and (or) non-typhoid, paratyphoid Salmonella (see Table 2).

[0051] 1. The interpretation, collection requirements and pretreatment methods of different test materials according to the needs of the clinical laboratory

[0052] 1) Patient's blood: Take 3-7ml of patient's venous blood by aseptic operation, immediately inoculate it in a commercial blood culture bottle for enrichment culture and at the same time inoculate xylose lysine deoxycholate agar plate (XLD) and Salmonella The enzyme chromogen...

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Abstract

The invention relates to a kit for separating and identifying salmonella, and a preparation and an application thereof, which are mainly used for solving the technical difficult problems of high omission ratio, complicated identification steps, higher cost and non-intuitive screening result of an existing salmonella separation method. The kit disclosed by the invention comprises general-purpose type non-selective enrichment broth, general-purpose type non-selective enrichment broth which is concentrated by 10 times, selenite sulfonamide green-sulfanilamide enrichment broth, a xylose lysine deoxycholate agar plate, a salmonella enzyme chromogenic agar plate, a salmonella biochemical identification tube, a hydrogen sulfide filter paper strip and an indole filter paper strip, and is characterized by further comprising improved Robert enrichment broth which comprises the following components: 13.58g of magnesium chloride, 7.2g of sodium chloride, 4.5g of papain digestion soybean peptone, 1.26g of KH2PO4, 0.18g of K2HPO4, 36mg of malachite green and 1000ml of distilled water. The kit disclosed by the invention is mainly designed for synchronous separation and identification of salmonella (including typhoid, paratyphoid and non-typhoid and paratyphoid salmonella) in enteric pathogenic bacteria.

Description

technical field [0001] The present invention is aimed at the simultaneous isolation and identification method of Salmonella (including typhoid, paratyphoid and non-typhoid, paratyphoid Salmonella) among intestinal pathogenic bacteria, especially relates to the typical colony morphology and simple Kit, preparation and application of combined biochemical and specific enzyme-substrate reaction assays for the identification of all Salmonella species (serotypes) including typhoid, paratyphoid and nontyphoidal, paratyphoid Salmonella. Background technique [0002] The current clear classification of Salmonella includes 2 type species, 6 subspecies, and nearly 2,500 serotypes, and those related to humans are mainly typhoid, paratyphoid, non-typhoid, and paratyphoid Salmonella (mostly belonging to subspecies I) , because the pathogenic characteristics and biological characteristics of Salmonella typhi and paratyphi are quite different from most non-typhoid and paratyphi Salmonella, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/42
Inventor 许学斌袁政安金汇明
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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