Bacteriophage composition and application of bacteriophage composition in medicine for preventing and treating chicken salpingitis

A salpingitis and bacteriophage technology, applied in the field of microorganisms, can solve the problems of high drug cost, unsatisfactory effect, and long treatment time, and achieve the effects of wide application, broadening the scope of sterilization, and enhancing physical fitness and disease resistance

Active Publication Date: 2020-06-12
QINGDAO PHAGEPHARM BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Chinese medicine has some shortcomings such as long treatment time, unsatisfactory effect, and high cost of medication.
At present, there is no safe and effective drug for the prevention and treatment of chicken salpingitis

Method used

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  • Bacteriophage composition and application of bacteriophage composition in medicine for preventing and treating chicken salpingitis
  • Bacteriophage composition and application of bacteriophage composition in medicine for preventing and treating chicken salpingitis
  • Bacteriophage composition and application of bacteriophage composition in medicine for preventing and treating chicken salpingitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The isolation and preparation of embodiment 1 phage

[0042] The fecal liquid sewage, chicken intestinal tract and liver samples in the present invention are collected from many chicken farms in Shandong Province.

[0043] 1. Isolation and identification of pathogenic bacteria

[0044] From a laying hen farm in Shandong, sick chickens with salpingitis were collected for dissection, and pathogenic bacteria were isolated from the oviducts. They were cultured in SS medium, and the pathogenic bacteria obtained were identified by microscopic examination and identified by corresponding PCR.

[0045] Results: On the SS medium, small off-white colonies with black hearts were isolated, with smooth and raised colony edges, which were identified as Salmonella by PCR with specific primers for Salmonella; red colonies with a slightly lighter color around them were also isolated on the SS medium, and colonies were tested by large intestine Escherichia coli was identified by PCR wit...

Embodiment 2

[0051] Morphological characteristics of embodiment 2 coli phage PD07 and Salmonella phage PYC04 Bacteriophage PD07 is observed under transmission electron microscope (see figure 1 ). The head of the phage has a polyhedral structure and has a slender tail. The long diameter of the phage head is about 86.67 nm, the transverse diameter is about 106.67 nm, and the tail is about 120 nm. According to the definition of the International Committee on Taxonomy of Viruses (ICTV), the morphology of bacteriophage PD07 conforms to the characteristics of Myoviridae and belongs to Myoviridae.

[0052] Phage PYC04 was placed under a transmission electron microscope for observation (see figure 2 ). The head of the phage has a polyhedral structure and has a slender tail. The long diameter of the phage head is about 66.67nm, the transverse diameter is about 60nm, and the tail is about 133.33nm. According to the definition of the International Committee on Taxonomy of Viruses (ICTV), the morp...

Embodiment 3

[0053] Whole Genome Analysis of Example 3 Phage PD07 and PYC04

[0054] The genomes of Escherichia coli phage PD07 and Salmonella phage PYC04 were respectively extracted and sequenced to obtain:

[0055] The genome size of phage PD07 is 40629bp, and phage PD07 is a double-stranded DNA virus. The proportions of the four bases of A, G, C, and T in the whole genome sequence are 23.81%, 23.38%, 26.51%, 26.38%, respectively, and G+ The C% content is 49.89%. The genome size of bacteriophage PYC04 is 41843bp, which is a circular double-stranded DNA. The proportions of the four bases of genome sequence A, G, C, and T are 25.03%, 24.94%, 24.56%, and 25.47%, respectively, and the content of G+C% is 49.50%.

[0056] According to RAST prediction analysis, the genome of phage PD07 contains 271 open reading frames (ORFs), and the genome of phage PYC04 contains 62 open reading frames (ORFs). Compared with 62 ORFs protein sequences of phage PYC04, phage PD07 contained 24 known encoded func...

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Abstract

The invention discloses a bacteriophage composition and an application of the bacteriophage composition in a medicine for preventing and treating chicken salpingitis. The bacteriophage composition comprises a coliphage PD07 and a salmonella bacteriophage PYC04. A preservation number of the coliphage PD07 is CGMCC No18865, and the coliphage PD07 is preserved in CGMCC on November 4, 2019; the preservation number of the salmonella bacteriophage PYC04 is CGMCC No18867, and the salmonella bacteriophage PYC04 is preserved in the CGMCC on November 4, 2019. According to the bacteriophage composition,the coliphage PD07 and the salmonella bacteriophage PYC04 are compounded to be used as a component for effectively preventing and treating the chicken salpingitis; and compared with a single lytic escherichia coli phage, the bacteriophage composition has advantages that a sterilization range is greatly widened; sterilization activity is improved; a laying rate of laying hens can be increased, andphysique and disease resistance of the laying hens are enhanced.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacteriophage and its application in medicine for preventing and treating chicken oviductitis. Background technique [0002] The common cause of chicken salpingitis is due to biological pathogenic bacteria infection, Escherichia coli and Salmonella are the most common pathogens. A white pus-like inflammatory exudate is common from the fallopian tubes, and the feathers below the anus are often contaminated by inflammatory substances. When the fallopian tubes are inflamed, the eggshells will become thinner, and the proportion of eggs with sandy shells, deformed eggs, and eggs with broken shells will increase, and the appearance of eggs will deteriorate. Salpingitis affects the quality of eggs, and in severe cases, inflammation spread and systemic infection can occur, causing chicken death and causing significant economic losses. [0003] In my country, in order to ensure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12Q1/70A61K35/76A61P15/00A61P31/14A01N63/40A01P1/00C12R1/92
CPCC12N7/00A61K35/76C12N2795/10121C12Q1/70A61P15/08A61P31/04A61P1/12A01N63/40A23K10/18A23K50/75C12N2795/10131C12N2795/10133C12N2795/10321C12N2795/10331C12N2795/10333A61K2300/00Y02A50/30
Inventor 潘强任慧英孙虎芝刘广芹葛超罗志祥
Owner QINGDAO PHAGEPHARM BIO TECH CO LTD
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