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Bacillus amyloliquefaciens SCAU-070 and application thereof

A SCAU-070, amylolytic spore technology, applied in the field of microbiology, can solve the problems of bacterial drug resistance, harm to humans and aquatic animals, and achieve the effects of reducing use, preventing or treating aquatic animal diseases, and promoting the growth of aquatic animals

Active Publication Date: 2020-11-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the large-scale misuse of antibiotics can cause severe bacterial resistance and also harm humans and aquatic animals

Method used

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  • Bacillus amyloliquefaciens SCAU-070 and application thereof
  • Bacillus amyloliquefaciens SCAU-070 and application thereof
  • Bacillus amyloliquefaciens SCAU-070 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and Identification of Bacillus amyloliquefaciens SCAU-070

[0033] 1. Experimental method

[0034] (1) Sampling: healthy Gifu tilapia was collected from the underground breeding room of the School of Animal Science, South China Agricultural University.

[0035] (2) Culture medium: liquid medium for enrichment (LB medium): 10 g peptone, 5 g yeast powder, 10 g NaCl, 1000 mL distilled water, pH 7.0-7.2.

[0036] Medium for separation: Add 1.5%-2% agar in LB medium.

[0037] (3) Isolation of Bacillus

[0038] After the surface of the tilapia was sterilized with cotton dipped in 75% alcohol, it was placed in an ultra-clean bench for dissection. Isolate the tilapia gut and squeeze out the gut contents using sterilized forceps and scissors.

[0039] Thoroughly triturate the tilapia gut contents with 1 mL of sterile water. After diluting 10 times, spread 0.1 mL of bacterial liquid on the prepared isolation medium (at least 3 copies of each medium), and ...

Embodiment 2

[0047] Antibacterial experiment of embodiment 2 Bacillus amyloliquefaciens SCAU-070

[0048] 1. Agar block method antibacterial experiment

[0049] (1) Experimental method

[0050] Bacillus amyloliquefaciens SCAU-070 was cultured at 37°C for 24 h with LB agar medium, and the agar block containing Bacillus amyloliquefaciens SCAU-070 cells was excised and placed on a test plate for indicating microorganisms. Four common aquatic animal pathogenic bacteria, Streptococcus agalactiae, Streptococcus iniae, Micrococcus luteus, and Vibrio parahaemolyticus, were used as indicator microorganisms, and the test plate with agar blocks and indicator microorganisms was incubated at 37°C for 27 hours , the antimicrobial activity was expressed as the diameter of the zone of growth inhibition (in mm), and each test was performed in triplicate.

[0051] (2) Experimental results

[0052] The bacteriostasis experiment result of Bacillus amyloliquefaciens SCAU-070 is shown in table 1, and the ant...

Embodiment 3

[0063] Example 3 The prebiotic effect of Bacillus amyloliquefaciens SCAU-070 on tilapia

[0064] 1. Experimental method

[0065] (1) Preparation of Bacillus amyloliquefaciens SCAU-070 added feed

[0066] Inoculate Bacillus amyloliquefaciens SCAU-070 in LB liquid medium, culture at 37°C for 24 hours, and then follow the fermentation process: glucose 42g / L, L-glutamic acid 14g / L, MgSO 4 0.5g / L, KCl 0.5g / L, KH 2 PO 4 1g / L, FeSO 4 0.15mg / L, MnSO 4 5.0mg / L, CuSO 4 0.16mg / L, pH 7.0, temperature 27°C, rotation speed 200r / min, inoculum size 4%, liquid volume 50mL / 250mL, cultured in a shaker for 36h. Then the fermentation broth was centrifuged at 8000rpm for 15min and the supernatant was discarded. In order to remove residual vegetative bodies, all the spores were transferred to lysozyme (4mg / mL) for 10min, and the treated spores were washed with sterile water and centrifuged at 8000rpm for 15min, and repeated once. The subsequent spores were heat treated in a water bath at...

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Abstract

The invention discloses Bacillus amyloliquefaciens SCAU-070 and application thereof. The Bacillus amyloliquefaciens SCAU-070 is obtained by separating and screening, the strain is deposited in the Guangdong Microbial Culture Collection Center on July 1, 2020, and the collection number is GDMCC No: 61074. The strain can significantly promote the growth of aquatic animals, inhibit the growth of aquatic animal pathogenic bacteria such as streptococcus agalactiae, streptococcus iniae, micrococcus luteus and vibrio parahaemolyticus in aquatic animal breeding water environment, improve the antioxidant ability of aquatic animal tissues, and regulate the content of cytokines in aquatic animal tissues; and therefore, the strain is of great significance in reducing the use of antibiotics and prohibited drugs, and has good application prospects and market value in the preparation of aquatic animal feed additives, microecological preparations for regulating the aquatic animal breeding water environment and medicines for improving the body immunity of aquatic animals.

Description

technical field [0001] The invention belongs to the technical field of microbiology. More specifically, it relates to a Bacillus amyloliquefaciens SCAU-070 and its application. Background technique [0002] According to the UN Food and Agriculture Organization's survey on the status of the global aquatic industry, the total fish production in 2016 was 171 million tons, of which about 88% (more than 151 million tons) were used for direct human consumption, a proportion that has increased in recent decades. This also means that people's consumption of aquatic products has reached an unprecedented high value and is likely to continue to rise. [0003] Among aquaculture species, tilapia is considered to be the most popular farmed fish in the world. Tilapia contributes 4.5 million tons per year to global food security and is a relatively cheap and high-quality protein source. However, mortality in farmed fish is often high due to disease outbreaks, especially during the higher ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/80A61K35/747A61P37/04A01N63/22A01P1/00C02F3/34C12R1/07C02F103/20
CPCA23K10/18A23K50/80A61K35/747A61P37/04A01N63/22C02F3/34C02F2103/20C12R2001/07C12N1/205Y02A40/81
Inventor 潘庆吴峰张晓勇
Owner SOUTH CHINA AGRI UNIV
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