11 results about "Indicator microorganisms" patented technology

A method and an arrangement, for detecting microorganisms on a surface are disclosed. At least a portion of the surface is covered with a sensor foil in an airtight manner. An oxygen-permeable layer of the sensor foil is doped with an oxygen indicator dye, loaded with oxygen and faces the at least one portion of the surface. An excitation light passes through an oxygen-impermeable at least partially transparent read-out carrier layer of the foil to the dye in the oxygen-permeable layer which is then excited by the excitation light. An emission of the oxygen indicator dye) transmitted through the carrier layer is detected by a detection element over or after a period of time (t). The emission of the oxygen indicator dye from the oxygen-permeable layer is indicative of the amount of oxygen consumed by microorganisms from the oxygen-permeable layer covering the at least one portion of the surface.

This invention discloses a method for ecological risk assessment of soil heavy metal compound pollution based on a joint species distribution model and counterfactual inference. A dual-model statistical framework of abundance and existence probability models is used, combined with AICc model selection, Cauchy combination test p-value merging, and FDR multiple hypothesis correction to screen indicator microbial groups. A joint species distribution model is constructed from the indicator microbial groups, and the posterior distribution of parameters is estimated using Bayesian MCMC. Based on the g-computation counterfactual inference framework, quantitative assessments of the average treatment effect of single metals, multi-metal interaction effects, single-metal ecological transformation risk index, and heavy metal comprehensive risk index are achieved. A Monte Carlo simulation is used to construct a natural fluctuation zero distribution to statistically determine the risk level. This invention realizes a paradigm shift from pollution concentration characterization to ecological effect-driven risk assessment, and is applicable to regional soil heavy metal compound pollution ecological risk assessment and management decisions.

The invention belongs to the technical field of culture media, and particularly relates to a method for evaluating a culture medium raw material soy peptone, which comprises the steps of antibacterial substance detection, microbial growth promotion effect evaluation and soy peptone quality judgment according to a comprehensive evaluation standard. According to the method, the antibacterial substances in the soy peptone are accurately measured through a protein sequencing method, and the quality of the soy peptone can be comprehensively analyzed from multiple dimensions by combining growth data of various indication microorganisms, so that the limitation of a single evaluation index is avoided, the accuracy is greatly improved, the evaluation result is more accurate and reliable, and the method is suitable for large-scale popularization and application. The experiment and production efficiency is improved, the production cost is reduced, and the resource waste is reduced.

The present application relates to the technical field of water quality monitoring, and discloses a water sample pollution inspection method guided by microorganism detection, comprising: applying a pulsed electric field to a biochemical response system to reduce the mass transfer resistance of the cell membrane of an indicator microorganism by using a physical pore-forming effect; injecting a fluorescent substrate and synchronously collecting fluorescence emission intensity and backscattering light intensity; correcting the amplitude of the fluorescence emission intensity by using the backscattering light intensity to eliminate physical shielding interference and obtain a pre-steady-state fluorescence emission sequence; intercepting a characteristic value of the change rate of the pre-steady-state fluorescence emission sequence to calculate an intrinsic catalytic turnover index representing the specific catalytic activity of intracellular enzymes, and determining the pollution degree of the water sample to be tested according to the offset of the index relative to a preset reference value; and the present application can quickly identify pollutants with transmembrane mass transfer disorders and eliminate the influence of endogenous activity fluctuations of microorganisms on the detection result by means of the cooperation of electric pore-forming intervention and calculation logic.

The invention discloses a preparation method of a microbial standard substance for characterizing the sterilization performance of hydrogen peroxide, and belongs to the technical field of biological detection.The preparation method comprises the following steps that S1, raw materials are selected, specifically, indicator microorganisms, a carrier, modified chitosan, modified mesoporous nano-silica and tannic acid are included; s2, preparing a composite protection solution: dissolving the modified chitosan in a phosphate buffer solution, and adding a tannic acid solution; then adding the modified mesoporous nano silicon dioxide; s3, preparing a spore protection suspension; s4, inoculating and drying the carrier; and S5, packaging and storing. The method solves the problems of inaccurate, unstable and unreliable mass value of the microbial standard substance in the existing hydrogen peroxide sterilization performance evaluation.

The application discloses a kind of microbial standard substance preparation methods of characterizing hydrogen peroxide sterilization performance, belong to biological detection technical field, comprising the following steps: S1, raw material selection: including indicating microorganism, carrier, modified chitosan, modified mesoporous nanometer silicon dioxide and tannic acid;S2, preparation composite protective solution: modified chitosan is dissolved in phosphate buffer solution, add tannic acid solution;Again add modified mesoporous nanometer silicon dioxide;S3, preparation spore protection suspension;S4, the inoculation and drying of carrier;S5, packaging and storage;The application solves the problem that microbial standard substance quality value is not accurate, unstable, unreliable in the existing hydrogen peroxide sterilization performance evaluation.

A system and method are provided for detecting aquatic microorganisms. The system comprises a nucleic acid extraction step for extracting nucleic acid from aquatic samples, a nucleic acid amplification step for amplifying the extracted genomic material, and a nucleic acid detection step for detecting the presence of amplified nucleic acids, indicating the presence of microorganisms. The method and system are applicable to both marine and freshwater environments and can be used for point-of-care detection of pathogenic and / or beneficial aquatic microorganisms. A nucleic acid amplification component comprising a hand-held isothermal heater to amplify the extracted nucleic acid, and a nucleic acid detection component configured to detect amplified nucleic acid. The hand-held isothermal heater includes a main body, a sample port configured, to receive a sample tube, a viewing screen, a heat block, and an electronics compartment. The system enables rapid and efficient detection of pathogenic and beneficial aquatic microorganisms.

Using deep metagenomic sequencing of individual gut microbiomes, coupled with techniques such as detailed long-term diet, fasting, and same-meal postprandial cardiometabolic blood markers analyses, intestinal microbes (defined by clusters of genomes referred to as species-level genome bins (SGBs)) have been identified as associated with different conditions and / or habits. Techniques are disclosed for using food guidance to intentionally alter the composition of the microbiome of an individual. Such alteration may include increasing the presence, abundance, or relative abundance of pro-health indicator microbe(s) and / or decreasing the presence, abundance, or relative abundance of poor health indicator microbe(s).

An apparatus and methods for detecting the presence of bacteria in a catheter are provided. According to various embodiments, an apparatus may include a housing having an aperture configured to orient a catheter through the housing and a compliant portion configured to move inwardly, at least one cutting element coupled to the compliant portion and configured to separate at least one catheter segment from the received catheter when the compliant portion is depressed, a media source having media configured to contact the at least one catheter segment after separating from the received catheter, and a detection element configured to indicate microbial growth in response to contact of the at least one catheter segment with the media.

Systems and devices for detection of and analysis a microbial species response to a treatment agent are provided. The system comprises a treatment agent that interacts with a microbial species to externalize a previously intracellular target biomolecule. The target biomolecule or a fragment thereof is an extracellular biomolecule target which is analyzed and detected thus indicating microbial susceptibility to the treatment agent. The system can be a rapid infection detection system for detecting a pathogen in a patient and provide treatment prediction for multiple treatment agents, such as antibiotics or other pharmaceutical agents. Devices with multiple treatment and detection channels are also provided. The devices can simultaneously indicate the response of a microbial species to multiple treatment agents and include a detection segment which visibly indicates the microbial response such that the device can be used in a clinical setting to design a treatment protocol for a patient.

Methods and systems for detecting unwanted microbes, and the origins thereof, in an environment are disclosed herein. The methods disclosed herein can be applied to a wide range of environments, including, but not limited to, food production facilities, office facilities, and public facilities. The methods comprise obtaining one or more samples from one or more locations in an environment, sequencing a plurality of nucleic acid sequences within the samples, identifying one or more indicator microbial signatures or origin-indicating microbial signatures within the plurality of nucleic acid sequences, comparing the indicator microbial signatures against one or more databases, and identifying one or more unwanted microbes, and / or the origins thereof, that correspond to one or more of the microbial signatures identified.