32 results about "Indicator microorganisms" patented technology

The invention discloses a disease prediction method for aquaculture based on microflora change. The method is characterized in that diseases are predicted and warned according to change of sum of relative abundances of featured microflora in aquatic water. The method specifically comprises the following steps: obtaining microflora information in a healthy water body sample and a diseased water body sample by high-throughput sequencing or other methods; screening healthy featured microflora and disease featured microflora which respectively indicate the healthy and diseased states; and then, respectively establishing a predication model to predict unknown samples by taking the relative abundance of microorganisms in a disease featured microbial combination and that of microorganisms in a healthy featured microbial combination as independent variables as well as taking the healthy states of the samples as dependent variables. The method has the advantages that the probability of diseases of aquaculture organisms can be predicted by fewer indicative microbial types, and the prediction accuracy is high.

A system for detecting a wide range of microbial organisms, including virus, and determining concentrations in near real-time to determine titer, without the requirement to grow micro-organisms includes an electrometer configured to measure photo-induced interfacial voltages and an electrode assembly with a substrate and at least one electrode on a surface of the substrate electrically coupled to the electrometer. An attachment factor is applied to an exposed surface of each electrode. The attachment factor is effective for interaction with the microbial organism. A transparent vessel for containing the electrolytic solution is provided. The microbial organism may be contained in the electrolytic solution or applied to the coated electrode before being submerged in the electrolytic solution. A light source is configured to controllably produce a flash of activating light directed through the transparent vessel at the electrode causing a sensible photo-induce interfacial voltage indicative of the microbial organism and titer. A corresponding method includes steps of preparing the electrode surfaces with an attachment factor and exposing the submerged electrode surfaces to a flash of activating light to induce interfacial voltages indicative of a determined microbial agent and titer.

The invention provides a microbial index evaluation method for the health condition of mangrove wetland sediment and belongs to the field of environmental monitoring. The method comprises the steps ofinvestigating the current situation of a mangrove wetland; determining indicator microorganisms; selecting ideal reference points; establishing a mangrove sediment sample collection method and determining microbial analysis methods, wherein the microbial analysis methods comprise a sample DNA extraction method, a PCR amplification method and a clone library construction method; selecting an indicator microorganism index evaluation factor and adopting a gene copy number of the indicator microorganisms, a diversity index and the proportion of dominant flora as evaluation factors; determining the weight Wi of the microorganism index evaluation factors; calculating an evaluation ratio Ii of the microbial index evaluation factors, and calculating an evaluation index P; determining the evaluation grade, and evaluating the health condition of the mangrove wetland sediment according to the evaluation grade. The provided microbial index evaluation method for the health condition of the mangrove wetland sediment can be used for accurately and effectively evaluating the health condition of the mangrove wetland sediment under field conditions and is wide in application range.

A method for checking the protection tool of pathogenic microbes includes culturing non-toxic indicator microbe, sampling the microbes passing through the protecting tool by use of a sampling device,filling aseptic liquid in a sample holding container of said sampling device, putting the protecting tool to be checked in the filter, adding indicator microbe, turn on vacuum pump, collecting the indicator microbes, and culturing them to determine the protective power of said protecting tool.

The invention relates to a color developing particle indicating the growth of microorganisms and a preparation method. The indicator particles of the invention have good stability, high sensitivity and low toxicity to microorganisms.

precancerous lesions, and subject having precancerous lesions and being treated with an anti-cancer agent; (2) isolating total microbial genomic DNA from the fecal samples to provide total microbial genomic DNA; (3) comparing the total microbial genomic DNA using fingerprint spectrum analysis; (4) identifying key fingerprint bands correlated with the effect of the anti-cancer agent; (5) identifying key microorganisms associated with the key fingerprint bands; (6) designing microbial sequence-specific primers and probes; and (7) determining the quantitative differences of the key microorganisms in fecal samples to identify an indicator microorganism for monitoring the effect of the anti-cancer agent.

The invention discloses a genetic engineering biological indicator used for biological monitoring of one or more sterilization effects. According to the genetic engineering biological indicator, foreign genes, including promoters, reporter genes and selectable maker genes, are guided into indicator microorganisms by means of genetic engineering. The selectable maker genes are positioned on the upstream of the inducible promoters, and the reporter genes are positioned on the downstream of the inducible promoters. In the absence of inducers, the selectable maker genes are expressed, the reporter genes are not expressed, and accordingly, successfully-transfected strains are screened. After exposure by sterilization factors, the indicator microorganisms contact with the inducers, and the reporter genes are expressed. Autofluorescence of the genetic engineering biological indicator can be achieved without specific enzyme substrates, and the genetic engineering biological indicator has the advantages of high signal-to-noise ratio, strong fluorescence signal, easiness in detection, high sensitivity and the like.

The testing system includes simulative microbe culture unit, microbe detection unit and microbe sampling unit with function for simulating breath. The sampling unit includes outer tube, filler and container for samples. The filler is flexible connected to top part of the outer tube, and the inner side of the filler is flexible connected to internal fixation board. The low end of the filler is setup inside container. There is an opening in sidewall of the outer tube. The opening is connected to a manual simulation lung. Non-poisonous or lesser poisonous bacteria, fungus or bacteriophage are used as microbiological indicators to simulate stronger poisonous bacteria such as SARS. Ventilation condition of respirator is simulated through manual lung. Thus, the testing system reflects real protecting effect.

The present invention provides a method for evaluating oral health, in particularly for evaluating a risk for suffering from periodontal disease, comprising: (1) providing an oral sample of a subject; (2) detecting at least two indicator microbes in the oral sample; and (3) analyzing amounts of the at least two indicator microbes; wherein when each of the amounts of the at least two indicator microbes is higher than a standard value for an amount of a corresponding indicator microbe, it indicates that the subject has a high risk for suffering from periodontal disease.

The invention relates to an indicator tube for indicating microbe growth and application thereof. As a general container, the indicator tube of the invention employs a drying method to conduct coloration for indicating storage and transportation of particles; so that indicating particles are maintained at a drying state be for usage, and influence of humidity on a redox indicator (e.g. tetrazole salt) is avoided, and the redox indicator is not susceptible to degradation or color changing. The invention also provides a kit for detecting microbe growth. The indicator tube of the invention is easily used, has good versatility, high sensitivity and low toxicity.

The present invention provides a marine sediment organic pollution rapid monitoring method which mainly includes the following steps: extracting sediment environment total DNA; using a primer for amplification of 16 s rDNA fragments; cloning and determining DNA sequence; comparing the obtained DNA sequence with Candidate division WS3 16 s DNA sequence (SEQ ID NO. 3) and Tenericutes 16 s DNA sequence (SEQ ID NO. 4); and analyzing Candidate division WS3 and Tenericutes microorganisms exist and content. The marine sediment organic pollution rapid monitoring method combines the advantages of the molecular biological technique and indicator biological method, organic pollutants (organochlorine pesticides and polychlorinated biphenyls (PCBS)) and indicator microorganisms (Candidate division WS3 and Tenericutes) of the organic pollutants are directly corresponded, marine complex organic matter polluted environment fast and exact monitoring can be achieved, key pollutant kind of comprehensive organic matter polluted environment can be identified, and the influence of pollution comprehensive effect on microbial community and the ecological system can be predicted.

The invention discloses a method for detecting the biological toxicity of atmospheric particulates. The method comprises the steps that two proteins SSA1 and HSP60 havingspecific sensitive response to oxidative stress of saccharomycetes are screened, a gene fluorescence probe is utilized to mark and control genes of the specific proteins of a living saccharomycetes body to make the living saccharomycetes body serve as an atmospheric particulate indicator microbe, and the biological toxicity of the atmospheric particulates is inversed by detecting the expression concentrations of different fluorescent proteins having specific wavelength in real time. The toxicity of atmospheric particulates is detected at the level of the single living saccharomycetes body, the method has the advantages of being high in sensitivity, low detection limit and the like, can be applied to thehuman health effectassessment of the atmospheric particulates and biological ingredients and measurement and real-time monitoring of air quality.

The invention relates to an industrial and urban mixed sewage stable operation control method. Activated carbon composite activated sludge and zeolite composite nitrobacterium in a target sewage treatment plant are used as indicator microorganisms to prepare microbial electrodes, and multichannel biosensors are adopted for monitoring toxicity of inflow water quality of the sewage treatment plant and water quality of an aerobic digester to provide basis for prevention and control of impact of toxic substances in industrial wastewater on a biological treatment system, so that energy saving, consumption reduction and operation stability of the industrial and urban mixed sewage treatment plant are guaranteed. The method is simple, cheap and convenient to operate, the defect that traditional chemical monitoring instrument analysis fails to objectively reflect microbial activity is overcome, and a promising popularization and application prospect is achieved.

The invention discloses a novel genetic engineering biological indicator. A foreign gene comprising a constitutive promoter, a reporter gene and a selectable maker gene is imported into an indicator microorganism through a gene engineering technique means, both the reporter gene and the selectable maker gene are located at the downstream of the constitutive promoter and are regulated and controlled by the upstream constitutive promoter, the selectable maker gene is used for screening a strain being successfully transfected, the reporter gene is used for indicating the growth of the microorganism after sterilization and exposure, and when the strain grows, the constitutive promoter is activated to promote the expression of the reporter gene and the selectable maker gene; according to the novel genetic engineering biological indicator provided by the invention, the constitutive promoter activated in the earlier stage of spore recovery growth is used for starting the expression of the foreign gene, remaining living spores can be quickly detected at the earlier stage of spore recovery growth, and the indicator is suitable for detection on the organism with one or more sterilization efficacies. The indicator provided by the invention has no need of any inductors or specific enzyme substrates, has a simple foreign gene system, is easy to operate and high in sensitivity and has a good industrial application prospect.

The invention discloses a method for rapidly identifying or screening microorganisms producing bacteriostatic active substances. The method comprises steps as follows: a solid culture medium culture dish (an LB culture medium or a PDA culture medium and the like) is prepared; tested microorganisms and indicator microorganisms are inoculated to the same culture dish under the sterile condition with a cross-streak method; after inoculation, the culture dish is put in a culture box for inverted culture under the condition of 26-40 DEG C for 12-240 h; whether the tested microorganisms produce the active substances for inhibiting the microorganisms is determined. By means of the method, whether the tested microorganisms produce the active substances for inhibiting the indicator microorganisms can be rapidly identified or the microorganisms producing the active substances for inhibiting the indicator microorganisms can be rapidly screened, the steps are simple, and the efficiency is high.

The present invention relates to a method for detecting the presence of water born pathogens and indicator microorganism including bacteria from water sample by selecting the target gene carried in template DNA by amplifying the target DNA using specific primers with biotinylated tag consist of all or a substantial part of 5′-CTGATCGAATGGCTGCCAGGCTCC-3′ and 5′-CAACCAGACGATAGTTATCACGCA-3′ and taq DNA polymerase to get desired biotinylated tagged probe followed by hybridization of biotinylated tagged probe with target gene in template DNA followed by enzyme coupled reaction.

The invention relates to an inspection method for a microbial drug sensitivity test by using a drug sensitivity indicator, which takes resazurin or soluble resazurin salt thereof as the drug sensitivity indicator and indicates a microbial drug sensitivity test result through a color and/or fluorescence signal. After the drug sensitivity indicator is combined with a drug sensitivity test, the sensitivity of the drug sensitivity test can be improved, the detection time of the drug sensitivity test can be shortened, and the drug sensitivity indicator has important clinical guidance significance and high application value.