33 results about "Indicator microorganisms" patented technology

A system for detecting a wide range of microbial organisms, including virus, and determining concentrations in near real-time to determine titer, without the requirement to grow micro-organisms includes an electrometer configured to measure photo-induced interfacial voltages and an electrode assembly with a substrate and at least one electrode on a surface of the substrate electrically coupled to the electrometer. An attachment factor is applied to an exposed surface of each electrode. The attachment factor is effective for interaction with the microbial organism. A transparent vessel for containing the electrolytic solution is provided. The microbial organism may be contained in the electrolytic solution or applied to the coated electrode before being submerged in the electrolytic solution. A light source is configured to controllably produce a flash of activating light directed through the transparent vessel at the electrode causing a sensible photo-induce interfacial voltage indicative of the microbial organism and titer. A corresponding method includes steps of preparing the electrode surfaces with an attachment factor and exposing the submerged electrode surfaces to a flash of activating light to induce interfacial voltages indicative of a determined microbial agent and titer.

The invention discloses a mask quality detection system and a detection method thereof. The detection system includes a simulated microorganism cultivation device and a microorganism detection device, and also includes a breathing simulation microorganism sampling device, and the breathing simulation microorganism sampling device includes an outer sleeve and an outer sleeve The top is movably connected with the funnel, the inner side of the upper end of the funnel is movably connected with the internal fixation plate, the lower end of the funnel is set in the sample container, and the side wall of the outer casing is provided with a side wall opening, which is connected with the artificial simulated lung. The advantages of this system are: Use non-toxic or less toxic bacteria or fungi or phages as indicator microorganisms to simulate clinically toxic pathogenic microorganisms such as lambda phages to simulate Sars virus, etc.; use artificial simulated lungs to simulate the breathing of masks used by humans Ventilation status, test the quality of the mask, and truly reflect the protective effect of protective equipment.

Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism. Corresponding kits are also provided.

The invention discloses a method for detecting target microorganisms. The method includes providing a culture device comprising a selective medium and a detection article comprising a first indicator system. The selective medium promotes the growth of indicator microorganisms. When an indicator microorganism is detected in a sample contacted with the medium, the detection article is contacted with the medium to detect the target microorganism.

Non-invasive methods for monitoring the effect of an agent for treating colorectal cancer, comprising (1) collecting fecal samples from healthy subjects, subjects having precancerous lesions, and subject having precancerous lesions and being treated with an anti-cancer agent; (2) isolating total microbial genomic DNA from the fecal samples to provide total microbial genomic DNA; (3) comparing the total microbial genomic DNA using fingerprint spectrum analysis; (4) identifying key fingerprint bands correlated with the effect of the anti-cancer agent; (5) identifying key microorganisms associated with the key fingerprint bands; (6) designing microbial sequence-specific primers and probes; and (7) determining the quantitative differences of the key microorganisms in fecal samples to identify an indicator microorganism for monitoring the effect of the anti-cancer agent.