Streptococcus iniae loop-mediated isothermal amplification kit and application thereof

A Streptococcus iniae, ring-mediated isothermal technology, applied in the field of microbial detection, can solve the problems of being unsuitable for grassroots and on-site detection, complex equipment, high cost, etc., and achieve the effect of simple result interpretation
CN104651518AInactive Publication Date: 2015-05-27GUANGXI VETERINARY RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI VETERINARY RES INST
Publication Date
2015-05-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a streptococcus iniae loop-mediated isothermal amplification kit and application thereof. The kit comprises LAMP primers, a 2*reaction buffer, a Bst DNA polymerase, a fluorescent visual detection reagent, ultra-pure water and a streptococcus iniae DNA template, wherein the LAMP primers comprise outer primers F3 and B33 and inner primers FIP and BIP. Specificity detection and sensitivity detection show that the LAMP detection method provided by the invention can be used for monitoring reaction in real time and detecting the copy number of streptococcus iniae quantitatively so that the detection result can be obtained rapidly and accurately and convenience is brought for simple and rapid detection of the streptococcus iniae.
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Description

technical field

[0001] The invention relates to the technical field of microbial detection, in particular to a loop-mediated isothermal amplification kit for rapid, visual and real-time quantitative detection of Streptococcus iniae and its application. Background technique

[0002] Fish streptococcosis is a disease caused by streptococcal infection, and the pathogenic bacteria belong to 3 genera and 7 species. Streptococcus pathogens include Streptococcus iniae, Streptococcus agalactme, Streptococcus paramastitis ( Streptococcus parauberis) and Streptococcus dysgalactiae; Lactococcus garvieae and Lactococcus piscium of the genus Lactococcus; Among them, Streptococcus iniae, Streptococcus agalactiae, Streptococcus paramastitis, Streptococcus dysgalactiae and Lactococcus griseri are the pathogenic bacteria of streptococcosis in warm water fish, while Lactococcus piscine and Salmonella are cold-water fish chains. The causative bacteria of coccidiosis.

[0003] At present, S...

Claims

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