Paralichthys olivaceus streptococcus iniae GAPDH series-connection multi-epitope polypeptide and application thereof

A Streptococcus iniae, multi-epitope technology, applied in the direction of fusion of polypeptides, peptides, bacteria, etc., can solve the problems of epitope vaccine reports and unsatisfactory effects, and achieve strong immune protection
CN110372797AActive Publication Date: 2019-10-25OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2019-10-25

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Abstract

The invention provides a paralichthys olivaceus streptococcus iniae GAPDH series-connection multi-epitope polypeptide and application thereof. The provided series-connection multi-epitope polypeptideis formed by binding four antigen epitope peptides through peptide joints, wherein the amino acid sequences of the four antigen epitope peptides are shown in SEQID NO:1-4 respectively. One specific amino acid sequence of the series-connection multi-epitope polypeptide is SEQID NO:8. The invention further provides a streptococcus iniae GAPDH multi-epitope vaccine prepared by using the series-connection multi-epitope polypeptide as an antigen and a vaccine adjuvant. The multi-epitope vaccine can provide strong immune protection for paralichthys olivaceus resistance to S.iniae infection, and theeffect is significantly higher than that of a GAPDH recombinant protein subunit vaccine and a S.iniae inactivated vaccine.
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Description

technical field

[0001] The invention belongs to the technical field of aquatic vaccines, and in particular relates to a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) tandem multi-epitope polypeptide of Streptococcus iniae and its application in preparing vaccines. Background technique

[0002] Streptococcus iniae (S. iniae) is a Gram-positive bacterium belonging to the genus Streptococcus of the family Streptococcus. Streptococcus iniae is widely distributed in marine and freshwater environments, and can infect a variety of marine and freshwater farmed fish, such as flounder (P.olivaceus), turbot (Scophthalmus maximus), red sea bream (Chrysophrys major), black sea bream (Sparus microcephalus) , Tilapia (Oreochromis nilotica), etc., can be infected from juvenile to adult fish, causing significant economic losses to the aquaculture industry. S. iniae can also infect humans, causing bacterial cellulitis, meningitis, endocarditis, and infectious arthritis. At present, antib...

Claims

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