Streptococcus iniae trivalent DNA vaccine and construction method thereof

A DNA vaccine, Streptococcus iniae technology, applied in the field of vaccinology, can solve problems such as lack of vaccines
CN102716499AInactive Publication Date: 2012-10-10INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2012-10-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of vaccinology, in particular to a Streptococcus iniae trivalent DNA vaccine and to a construction method thereof. The method comprises the steps of using Streptococcus iniae G26 as a template, allowing the primers of F1 / R1, F2 / R2 and F3 / R3 to carry out PCR amplification respectively, allowing the product and a vector pCN3 plasmid to be subjected to connection and transformation, and allowing the plasmid to be subjected to equivalent volume mixing to obtain the trivalent DNA vaccine. The efficiency of the protection of the vaccine obtained by the invention against Streptococcus iniae infection is more than 90% in one month or two months after immunization.
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Description

technical field

[0001] The invention relates to the field of vaccinology, in particular to a Streptococcus iniae trivalent DNA vaccine and a construction method thereof. Background technique

[0002] Streptococcus iniae is a Gram-positive bacterium that can infect a variety of cultured seawater and freshwater fish, including flounder, tilapia, grouper, and American redfish. considered to be a major fish pathogen. In addition, Streptococcus iniae is a zoonotic pathogen that can infect humans through sick animals, so the research on its immune control is of great significance. At present, due to the lack of effective vaccines, the prevention and treatment of streptococcal dolphin disease in my country mainly relies on the use of drugs such as antibiotics. In this case, the development of an effective vaccine against this bacteria has become an urgent need for the aquaculture industry. Contents of the invention

[0003] The purpose of the present invention is to provide a ...

Claims

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