SRAP molecule labeled primer for Megalobrama amblycephala family identification, method thereof, and application of primer

A technology of molecular markers and bream, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of complex operation, long time-consuming, difficult application, etc., and achieve simple operation and cost saving , easy-to-sequence effects
CN104293907AInactive Publication Date: 2015-01-21HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2015-01-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an SRAP molecule labeled primer for Megalobrama amblycephala family identification, a method thereof, and an application of the primer. The SRAP molecule labeled primer is selected from one of the following primer combinations: Me2 / Em18, Me3 / Em19, Me9 / Em13, Me9 / Em15, Me9 / Em16, Me11 / Em4, Me11 / Em15, Me11 / Em18, Me12 / Em13 and Me12 / Em16, and the sequences of all the primers are respectively shown in a sequence table. The disclosed SRAP molecular marker primer is used to identify the Megalobrama amblycephala family by carrying out SRAP molecule labeling of the Megalobrama amblycephala family, and a band obtained in the invention has the advantages of clearness, good polymorphism, high repeatability, simple operation and broad application prospect.
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Description

technical field

[0001] The invention relates to an SRAP molecular marker primer used for identification of bream family, and belongs to the technical field of aquatic animal molecular biology. Background technique

[0002] Related sequence amplified polymorphism (sequence related amplified polymorphism, SRAP) is a marker system developed based on traditional PCR, which amplifies the reading frame regions (Open Reading Frames, ORFs) of genes through unique primer design. In this method, the 17bp forward primer specifically amplifies the exon, and the 18bp reverse primer specifically amplifies the intron and promoter region. Polymorphisms occur due to differences in the length of the interval between introns and promoters within or between species. Compared with other methods, SRAP molecular markers are characterized by simplicity, rapidity, stability, good reproducibility, medium yield, co-dominant markers, and easy cloning and sequencing of target fragments. They are widely...

Claims

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