Method for identifying foxtail millet variety by adopting SSR molecular marker technique and application

A molecular marker and millet technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high cost, long cycle, restricting the accuracy of identification, etc., and achieve the effect of saving time and better effect.

Active Publication Date: 2016-03-09
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Although this method is simple and economical, it has a long cycle, high cost, and is limited by seasons, and the performance of many characters is affected by cultivation measures and environmental factors, which restricts the accuracy of identification.

Method used

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  • Method for identifying foxtail millet variety by adopting SSR molecular marker technique and application
  • Method for identifying foxtail millet variety by adopting SSR molecular marker technique and application
  • Method for identifying foxtail millet variety by adopting SSR molecular marker technique and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the establishment of method

[0026] 1. Prepare millet materials: Gaizhou red, duck beak, big head yellow, big white hair, old laibian, red sticky valley, knife handle, gogenhong, money string, big green seedlings, yellow sticky valley, red millet, yellow grain 15 kinds of millet materials, such as duck mouth, big grain yellow, and red seedling valley, are planted in the greenhouse; take the young millet sprouts about 10d-15d after germination, freeze them in liquid nitrogen and put them in a -80°C ultra-low temperature refrigerator for later use; 15 kinds of millet varieties The information is shown in Table 1.

[0027] Table 115 Millet Variety Information

[0028] serial number

[0029] 2. Millet DNA extraction: Extract the DNA of various millet materials with a plant genome kit, and use 1% agarose gel electrophoresis to detect the DNA purity; dilute the DNA concentration of each material to 50ng / μL, and place it at -20 ℃ in the refrigerator ...

Embodiment 2

[0050] This embodiment provides a method for identifying millet varieties using SSR molecular marker technology, comprising the following steps:

[0051] 1. Prepare millet materials: Plant 45 kinds of millet materials in the greenhouse, including big-leaf yellow grain, purple stalk knife handle, money string tight grain, four-inch red root, red root rope head, thin-skinned white grain, and hen's beak; take germination After 10d-12d, the young millet sprouts were frozen in liquid nitrogen and stored in a -80°C ultra-low temperature refrigerator for later use; the information of the 45 millet varieties is shown in Table 7.

[0052] Table 745 Millet Variety Information

[0053]

[0054]

[0055] 2. Millet DNA extraction: Extract the DNA of various millet materials with a plant genome kit, and use 1% agarose gel electrophoresis to detect the DNA purity; dilute the DNA concentration of each material to 50ng / μL, and place it at -20 ℃ in the refrigerator for later use;

[005...

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Abstract

The invention discloses a method for identifying a foxtail millet variety by adopting an SSR molecular marker technique and application, and belongs to the technical field of plant variety identification. The method comprises the steps that foxtail millet DNA is extracted, a PCR amplification reaction is performed through the foxtail millet DNA and 5 pairs of SSR core primers, and after electrophoresis detection is performed, the foxtail millet variety is identified according to an electrophoresis detection result; the nucleotide sequences of the SSR core primers are shown as SEQ ID NO.1-10. According to the method, the foxtail millet variety can be effectively distinguished, and an important way is supplied to further identification of a large number of the foxtail millet varieties.

Description

technical field [0001] The invention relates to a method and application for identifying millet varieties using SSR molecular marker technology, and belongs to the technical field of plant variety identification. Background technique [0002] Millet is a crop of the genus Setaria in the Gramineae family. It has physiological characteristics such as short growth period, drought tolerance, and barren tolerance. It is widely distributed in the temperate and tropical regions of Eurasia. It is one of the main cultivated crops in the middle and upper reaches of the Yellow River in China. At the same time, the nutritional value of millet is extremely high, rich in amino acids, proteins, vitamins and trace elements such as calcium, copper, iron, zinc, selenium, iodine, magnesium, etc., and its nutritional and health effects are also very significant. For many years, breed identification was based on morphological markers. Although this method is simple and economical, it has a long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 张东杰王颖沈琰杨义杰孙大庆张桂芳
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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