SNP molecular marker relating to peach tree bleeding disease resistance

A technology of peach gummosis and molecular markers, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., to achieve the effects of improving breeding efficiency, high accuracy, and high degree of separation

Active Publication Date: 2017-05-10
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

[0007] At present, the development of SNP markers based on whole-genome resequencing and the construction of high-density SNP chips have been applied to the synthesis of peach fruit aroma substances and the genetic mechanism of chilling injury. The molecular mechanism of resistance to gummosis in different peach varieties still remains to be studied. As a blank, it is necessary to use molecular breeding methods to mine and locate disease-resistant genes, select parents, and provide a basis for the selection of new disease-resistant varieties

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  • SNP molecular marker relating to peach tree bleeding disease resistance
  • SNP molecular marker relating to peach tree bleeding disease resistance
  • SNP molecular marker relating to peach tree bleeding disease resistance

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Embodiment Construction

[0041] The present invention will be further described below in conjunction with specific examples.

[0042] Embodiment The acquisition of the closely linked SNP molecular markers of the QTL associated with peach tree gummosis resistance comprises the following steps:

[0043] 1) Build F 1 hybrid population

[0044] F 1 Finally, 80 hybrid offspring were obtained, and the offspring populations were segregated for gummosis traits.

[0045] 2) Genomic DNA extraction

[0046] Use the Qiagen DNeasy mini Kit kit to extract genomic DNA from 2 peach parents and 80 hybrid offspring, and follow the method provided in the kit, as follows:

[0047] ①Weigh 0.1g of frozen leaves, quickly grind them in liquid nitrogen, and place them in a 2ml sterilized centrifuge tube.

[0048] ② Add 400 μl AP1 buffer solution and 4 μl RNase A to the above centrifuge tube, vortex and mix well, bathe in 65°C water for 10 minutes, and mix up and down 2-3 times.

[0049] ③ Add 130 μl AP2 buffer solution to...

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Abstract

The invention discloses an SNP molecular marker relating to the peach tree bleeding disease resistance. The SNP molecular marker which has close linkage with the peach tree bleeding disease resistance related QTL is obtained by locating a peach tree bleeding disease resistance gene locus, and is located on the sixth chromosome of a peach; the locus is SNP_IGA_627726; the SNP locus is obviously related to the morbidity of the bleeding disease of different types of peaches and is high in polymorphism; a novel authentication method is provided for parent selection in the early stage and generation selection in the late hybridization stage in a bleeding disease resistance species selection process; the accuracy of bleeding disease resistance detection can be improved, the breeding period can be shortened, the breeding efficiency is improved, and implementation of seed selection of a new bleeding disease resistance species of the peach tree in a high temperature and high humidity district is accelerated.

Description

technical field [0001] The invention belongs to the technical field of peach tree breeding, and in particular relates to a SNP molecular marker related to peach tree gummosis resistance. Background technique [0002] Peach is a Rosaceae drupe fruit tree native to western my country, and it is also one of the most widely cultivated fruit trees in the world. Peach gummosis is one of the important factors affecting the healthy and orderly development of peach industry, which has caused serious harm to peach production in my country. [0003] Peach tree gummosis is caused by the infection of the fungus Botryosphaeria dothidea, which mainly occurs on the trunk and side branches of the tree, and develops into fruiting branches and fruits when the disease is severe. In the early stage of the disease, light yellow transparent glue flows out of the lenticels, and after the glue is solidified, it gradually turns reddish brown, the diseased part is slightly swollen, the cortex and xyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李雄伟叶正文周京一高中山贾惠娟苏明申杜纪红周慧娟
Owner SHANGHAI ACAD OF AGRI SCI
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