Kit for detecting APOE (Apoliprotein E) gene of Alzheimer disease

A technology of gene detection and detection reagents, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of increasing the difficulty of diagnosing the etiology, and achieve the effect of promoting the development of new drugs and high sensitivity

Inactive Publication Date: 2017-12-08
苏州康吉诊断试剂有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0003] With the continuous deepening of research, more and more risk factors have been discovered, which has enabled a deeper understanding of the cause of Alzheimer's disease, but at the same time it has also increased the difficulty of clinical diagnosis of the cause

Method used

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  • Kit for detecting APOE (Apoliprotein E) gene of Alzheimer disease
  • Kit for detecting APOE (Apoliprotein E) gene of Alzheimer disease
  • Kit for detecting APOE (Apoliprotein E) gene of Alzheimer disease

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Embodiment Construction

[0045] The specific embodiments of the present invention are described in further detail below.

[0046] (1) Collection of blood samples and extraction of genomic DNA

[0047] Genomic DNA was prepared according to the following method.

[0048] (1) Add 1.0ml of red blood cell lysate to a clean 1.5ml centrifuge tube, then add 200μl of anticoagulated whole blood, mix well, and centrifuge at 8000rpm for 2 minutes within ten minutes; suck up the upper hemoglobin solution with a sample gun and use a shaker The precipitate is fully popped and mixed; the digestion solution is prepared according to the ratio of STE buffer: Tris-Hcl buffer: proteinase K=150:30:15, add 200μl of digestion solution to each tube, mix well, and digest at 50°C for 1 hour; add The Tris-Hcl extract and absolute ethanol are thoroughly mixed upside down and transferred to a centrifugal adsorption column. Centrifuge at 8000rpm for one minute and discard the filtrate; add 600μl of 70% ethanol to the centrifugal adsorpti...

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Abstract

The invention discloses a kit for detecting an APOE (Apoliprotein E) gene of Alzheimer disease, and belongs to the technical field of biological detection. The kit disclosed by the invention comprises a unit PCR detection reagent; the unit PCR detection reagent comprises a buffer solution, protease K, magnesium chloride stock solution, a primer, a probe and a polymerase; the primer is a primer pair of a reagent for detecting rs429358 and rs7412 gene segments of the APOE gene; and the kit for detecting the APOE gene of Alzheimer disease is enough to measure the polymorphism of the APOE gene by only a small amount of DNA samples based on a new mutational site related to the APOE of the APOE gene, and can be used in a gene diagnosis method for measuring the polymorphism of the APOE-related gene.

Description

Technical field [0001] A kit belongs to the field of biological detection technology, and mainly relates to a kit for the APOE gene detection of Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a common disease in the elderly, with an incidence rate of about 5% over the age of 60, and the incidence has risen sharply with age, and has become a major disease that seriously threatens the quality of life of the elderly one. According to statistics, AD patients are expected to reach 25 million by 2050, which will become an important constraint on family and social development. Therefore, AD research is a major issue in the world of biomedicine. [0003] With the continuous deepening of research, more and more risk factors have been discovered, which has enabled a deeper understanding of the causes of Alzheimer's disease, but also increased the difficulty of clinical diagnosis of the cause. Among them, genetic factors play an important role in the pathoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 邵敏华黄迅威
Owner 苏州康吉诊断试剂有限公司
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