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Chrysanthemum salt-tolerance associated molecular marker and obtaining method and application thereof

A molecular marker and salt-tolerance technology, which is applied in the field of salt-tolerance-related molecular markers of chrysanthemum and its acquisition, and achieves the effects of short research period, broad genetic basis and high positioning accuracy

Active Publication Date: 2015-12-02
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the precise mapping and cloning of molecular markers associated with salt tolerance in chrysanthemums have not yet been reported.

Method used

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  • Chrysanthemum salt-tolerance associated molecular marker and obtaining method and application thereof
  • Chrysanthemum salt-tolerance associated molecular marker and obtaining method and application thereof
  • Chrysanthemum salt-tolerance associated molecular marker and obtaining method and application thereof

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Embodiment Construction

[0042] The present invention will be further described below in conjunction with the examples, and the experimental methods that do not specify specific conditions in the following examples generally follow the known methods in the art.

[0043] (1) Acquisition of test materials and their salt tolerance identification phenotype data

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Abstract

The invention discloses a chrysanthemum salt-tolerance associated molecular marker and an obtaining method and application thereof. The obtaining method comprises the steps that a, salt-tolerance identification is performed for two consecutive years; b, chrysanthemum variety population structure analysis is performed; c, chrysanthemum variety genetic relationship analysis is performed; d, the molecular marker closely associated with chrysanthemum salt-tolerance is determined. According to the obtaining method, genome-wide molecular marker scanning is performed on 159 cut-flower chrysanthemum varieties by utilizing the three molecular marker technologies of SRAP, SCoT and EST-SSR at the same time, the number of obtained dyed strips is large, the dyed strips are good in polymorphism and high in reproducibility, and the molecular marker which is closely associated with salt-tolerance can be obtained. Three marker loci detected through the obtaining method are remarkably associated with salt-tolerance of varieties and provide a certain reference for chrysanthemum salt-tolerance molecular marker-assisted selection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a molecular marker associated with salt tolerance of chrysanthemums, an obtaining method and application thereof. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium Ramat.) originates in China and is a perennial herbaceous plant of the genus Chrysanthemum in the family Asteraceae. There are many varieties of chrysanthemum, abundant germplasm resources, and rich genetic variation information. Soil salt damage has always been an important factor affecting agricultural production, and secondary salt damage caused by protected cultivation has attracted more and more attention. Cut chrysanthemums are planted in greenhouses. During the cultivation and management process, chemical fertilizers are applied but cannot be fully leached by rainwater. In addition, the continuous evaporation of soil surface water will cause salt accumulation in the soil of the facility, which wil...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12N15/82C12Q1/68A01H5/00
Inventor 陈发棣李丕睿苏江硕张飞王海滨蒋甲福房伟民
Owner NANJING AGRICULTURAL UNIVERSITY
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