Molecular marker of major QTL, qTLA-9 for regulating angle of tilt of paddy rice leaves, and application thereof

A technology of molecular markers and leaf inclination angle, which is applied in the field of rice breeding and molecular biology, can solve the problem of incomplete understanding of genes related to the regulation of rice leaf inclination angle, and achieve the effects of improving breeding efficiency, high map density, and high LOD value

Inactive Publication Date: 2019-05-21
ZHEJIANG NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

There are many studies on the genes related to the regulation of rice leaf inclination angle, which will be of great help to further research on its molecular mechanism. However, many studies focus on one or a few genes, and the current understanding of the genes related to the regulation of rice leaf inclination angle is still incomplete.

Method used

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  • Molecular marker of major QTL, qTLA-9 for regulating angle of tilt of paddy rice leaves, and application thereof
  • Molecular marker of major QTL, qTLA-9 for regulating angle of tilt of paddy rice leaves, and application thereof
  • Molecular marker of major QTL, qTLA-9 for regulating angle of tilt of paddy rice leaves, and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1, acquisition of experimental materials

[0046] The rice variety Huazhan has a relatively large leaf inclination angle. Huazhan was used as the donor parent and the local rice variety Reyan was used as the recipient parent to construct RILs by crossing. , until the phenotypes of offspring strains do not segregate), and finally 134 stable genetic strains were obtained (F13, all strains were phenotypically stable), such as figure 1 .

[0047] Select 60 seeds (F13) of the parent and each strain, soak the seeds for 2 days after surface disinfection, wrap them in a moist towel, put them in a 37°C incubator for 48 hours, and select the seeds with consistent dew and whiteness for sowing. After 30 days, the parents with similar growth conditions and 24 seedlings of each line were selected for transplantation. All rice materials were planted in the experimental field of the School of Chemistry, Zhejiang Normal University, Jinhua City, Zhejiang Province, and were ma...

Embodiment 2

[0048] Embodiment 2, leaf inclination angle data measurement

[0049] At the tillering stage, 5 tillers were randomly selected from each line, and the vertical inclination angles between the veins and stems of the three leaves were measured with a protractor. Record the data of each group and calculate the average value.

[0050] figure 2 Among them, RY represents the rice variety Reyan, and HZ represents the rice variety Huazhan; according to figure 2 It can be known that the data of the inclination angle of the inverted trefoil showed a continuous normal distribution and a wide range, and there were many super-parents, showing the genetic characteristics of quantitative traits.

Embodiment 3

[0051] Embodiment 3, QTL location analysis

[0052] Using the genetic map constructed by a large number of SNP and Indel markers developed by the laboratory in the early stage, the quantitative trait locus (QTL) interval mapping of the inclination angle of the rice leaf is carried out, and the markers and quantitative trait tables of the entire genome are analyzed by R-QTL professional software QTLs were mapped to corresponding positions in the linkage group one by one, and their genetic effects were estimated. If a molecular marker with LOD>2 is detected, it is considered that there is a QTL between the two markers corresponding to the highest LOD value ( image 3 ). In the whole genome, we found a major QTL located between the Indel Tal-1 marker and the Indel Tal-2 marker on chromosome 9, with a genetic distance of 74.80cM-98.33cM and a physical distance of 17448080bp-22938953bp, and Named qTLA-9 ( Figure 4 ).

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Abstract

The invention discloses a major QTL, qTLA-9, which is used for regulating the angle of tilt of paddy rice leaves, and locates between the mark Indel Tal-1 and on Indel Tal-2 on the ninth chromosome, and the genetic distance is 74.80 cm-98.33 cm, the physical distance is 17448080 bp-2293 8953 bp. 2293 8953 bp. The invention also provides a mark Indel of the major QTL, qTLA-9, which is used for regulating the angle of tilt of paddy rice leaves; the invention also provides the application of the major QTL: by developing the molecular marker closely linked to the major QTL, the test result that whether there are leaf inclination-related QTLs in rice varieties or strains can be attained, thereby speeding up the breeding process of excellent rice varieties.

Description

technical field [0001] The invention belongs to the technical field of rice breeding and molecular biology, and specifically relates to a main effect QTL related to rice leaf inclination angle and application thereof. Background technique [0002] With the increase of world population and the decrease of arable land, rice is one of the main food crops in the world, and its per unit yield needs to be further improved on the original basis. Donald first proposed the concept of ideal plant type, that is, the ideal plant type that can maximize the photosynthetic efficiency of plants (Leng Yujia et al., 2014). Since then, many scholars at home and abroad have conducted a lot of research on the ideal plant type in order to achieve a new breakthrough in rice yield. Leaf inclination, the angle formed by leaves and stems, is an important agronomic trait and an important component of ideal plant architecture in rice. The morphological characteristics of "ideal rice" proposed by Mats...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 周纯饶玉春王跃星戴志俊焦然金哲伦王予烨叶珺邓琳杰林雪章怡兰
Owner ZHEJIANG NORMAL UNIVERSITY
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