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Multiple-effect QTL related to regulation and control of rice amylose content and gel consistency, molecular markers and application

An amylose content and molecular marker technology, applied in the fields of rice breeding and molecular biology, can solve the problem of slow progress in rice quality improvement, and achieve the effects of convenient selection of rice progeny, accelerated breeding process, and convenient selection.

Active Publication Date: 2020-07-10
CHINA NAT RICE RES INST +1
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  • Abstract
  • Description
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Problems solved by technology

[0005] At present, due to the complexity of rice quality inheritance and the limitations of traditional breeding methods, the progress of rice quality improvement is slow

Method used

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  • Multiple-effect QTL related to regulation and control of rice amylose content and gel consistency, molecular markers and application
  • Multiple-effect QTL related to regulation and control of rice amylose content and gel consistency, molecular markers and application
  • Multiple-effect QTL related to regulation and control of rice amylose content and gel consistency, molecular markers and application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0043] 1. Acquisition of experimental materials

[0044] The rice variety Huazhan has low amylose content and gel consistency. Huazhan was used as the donor parent and the local rice variety Reyan No. 2 was used as the recipient parent to construct RILs by hybridization. 1 Bagged individual plants were inoculated until the phenotypes of the progeny lines did not segregate), and finally 120 stable genetic lines were obtained (F 13 , all strains are phenotypically stable), such as figure 1 shown.

[0045] Select the parents and the seeds of each line (F 13 ) of 60 grains each, soak the seeds for 2 days after surface disinfection, then wrap them in a moist towel, put them in a 37°C incubator for 48 hours, and select the seeds with consistent dew and whiteness for sowing. After 30 days, the parents with similar growth conditions and 24 seedlings of each line were selected for transplantation. All rice materials were planted in the experimental field of the School of Chemistry,...

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Abstract

The invention relates to the technical fields of rice breeding and molecular biology, and discloses multiple-effect QTL related to regulation and control of rice amylose content and gel consistency, molecular markers and application. The multiple-effect QTL is named as qAC-GC-1 and is located on a DNA fragment of chromosome 4, the genetic distance is 10.1 cM to 12.2 cM, the physical distance is 4207206 bp to 4766887 bp, and the LOD values of the amylose content and the gel consistency are 6.03 and 5.48 respectively. The molecular markers are respectively named as Indel Tal-1 and Indel Tal-2. The multiple-effect QTL can effectively increase the amylose content and the gel consistency of rice, can accelerate the cultivation of rice with moderate amylose content and high gel consistency in the molecular assisted breeding process of rice, and optimizes the quality of rice. The method is simple, convenient, feasible, safe, effective, beneficial to improving the rice quality and suitable forlarge-scale popularization and application.

Description

technical field [0001] The invention relates to the technical fields of rice breeding and molecular biology, in particular to multi-effect QTLs, molecular markers and applications related to regulating rice amylose content and gel consistency. Background technique [0002] Rice is the main food crop. With the development of science and technology and the improvement of living standards, people have put forward higher requirements for the inspection and evaluation system of rice quality. In the evaluation system of rice, the main reference data are the appearance, processing, cooking, nutrition and taste of rice. Among them, cooking quality is the most important item, and its characteristics are mainly evaluated by the following three physical and chemical indicators, namely amylose content (Amylose Content, AC), gel consistency (Gel Consistency, GC) and gelatinization temperature (Gelatinization Temperature, GT ), the most important of which is the amylose content. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王跃星饶玉春李三峰朱旭东林晗胡萍焦然
Owner CHINA NAT RICE RES INST
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