Method for discriminating Chinese milk vetch variety by using SSR (Simple Sequence Repeat)fingerprint spectrum

A fingerprint and vetch technology, applied in the field of molecular biology, can solve the problems of identification of difficult varieties and high hybridization rate, and achieve the effect of high allelic diversity and high information content.

Inactive Publication Date: 2011-02-16
INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

Due to the cross-pollination of milk vetch, the hybridization rate is high, and it is difficult to accurately identify the species only by morphological observation and growth characteristics.

Method used

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  • Method for discriminating Chinese milk vetch variety by using SSR (Simple Sequence Repeat)fingerprint spectrum
  • Method for discriminating Chinese milk vetch variety by using SSR (Simple Sequence Repeat)fingerprint spectrum
  • Method for discriminating Chinese milk vetch variety by using SSR (Simple Sequence Repeat)fingerprint spectrum

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Participating milk vetch varieties: 1: Minzi No. 1; 2: Xinyang species; 3: Ningbo Daqiao species; 4: 8324411; 5: Minzi No. 6; 6: 8410441; 9: Yujiang Daye seeds; Minzi 1, 8324411, Minzi 6, 8410441, 8487711 were provided by Fujian Academy of Agricultural Sciences, Xinyang seeds were provided by Henan Xinyang Agricultural Science Research Institute, Yujiang Daye Seeds were provided by Jiangxi Academy of Agricultural Sciences, Yijiang seeds were provided by Anhui Academy of Agricultural Sciences, and Ningbo Daqiao seeds were provided by Zhejiang Academy of Agricultural Sciences.

[0033] Second, the identification results of SSR markers:

[0034] 1. The band patterns of the tested milk vetch samples formed by the amplification of primers designed for different SSR sites: Figure 7 For primer EG6-54 ( Figure 7 middle right) and primer EG6-167 ( Figure 7 Middle left) Fingerprint band patterns formed in the range of 75bp-300bp for 9 varieties of milk vetch tested (M is...

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Abstract

The invention provides a method for discriminating Chinese milk vetch varieties by using an SSR (Simple Sequence Repeat)fingerprint spectrum. A specific SSR site and bilateral primer sequences thereof (SEQ.NO.1-18) in a Chinese milk vetch genome, and a fingerprint spectrum which is obtained by carrying out PCR (Polymerase Chain Reaction) amplification on screened primers can be used for discriminating Chinese milk vetch varieties. The discriminating method mainly comprises the following steps of: extracting Chinese milk vetch total DNA (Deoxyribonucleic Acid); screening the specific SSR site and designing primers from genome DNA by using magnetic-bead enrichment method; and finally obtaining 6 SSR site primer pairs which can form obvious banded difference by carrying out the PCR amplification on different varieties of different Chinese milk vetch. The constructed fingerprint spectrum can be effectively used for discriminating Chinese milk vetch varieties.

Description

technical field [0001] The invention belongs to the field of molecular biology, and more specifically relates to a method for identifying varieties of green manure crop milk vetch by using SSR fingerprints. Background technique [0002] Milk vetch (scientific name: Astragalus sinicus ) is a plant of the genus Astragalus in the family Fabaceae. It is an annual or perennial herb. Also known as safflower grass, tilt shake. It is widely distributed in 25-33 degrees north latitude, and mostly grows by streams, hillsides and humid places. China has planted it on a large scale in the middle and lower reaches of the Yangtze River as early as the Ming and Qing Dynasties. The main root of milk vetch is relatively fat, generally 40 to 50 cm into the soil, and the lateral roots are shallower. Root nodules can be formed on the main root, lateral root and fine root of the surface of milk vetch, most of which are on the lateral root. The stem is cylindrical, hollow, tender and juicy,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
Inventor 林新坚陈坚张辉陈济琛朱炳耀
Owner INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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