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Screening and application of solanum melongene SSR (Simple Sequence Repeats) molecular marker core primers

A core primer, molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of large genetic distance and narrow genetic basis.

Active Publication Date: 2019-12-31
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

Lu Ting et al. analyzed the genetic diversity of 66 eggplant materials with 25 pairs of SSR primers, and concluded that the genetic distance between eggplant species is relatively large, and the genetic basis within cultivated species is relatively narrow.

Method used

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  • Screening and application of solanum melongene SSR (Simple Sequence Repeats) molecular marker core primers
  • Screening and application of solanum melongene SSR (Simple Sequence Repeats) molecular marker core primers
  • Screening and application of solanum melongene SSR (Simple Sequence Repeats) molecular marker core primers

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Embodiment

[0030] The present embodiment relates to eggplant SSR molecular marker core primer and screening thereof, and the application in the identification of phylogenetic relationship; specifically as follows:

[0031] 1 Materials and methods

[0032] 1.1 Materials

[0033] The 133 pairs of SSR primers used in this experiment were synthesized by Shanghai Sangon Biotechnology Co., Ltd. In order to screen out the primers with higher polymorphism from 133 pairs of primers, 106 eggplant varieties were selected according to fruit shape, fruit rib (with / without), peel color (white / green / purple), fruit surface stripes, leaf 12 varieties with large differences (see Table 2) were selected for the 6 traits of color and fruit longitudinal diameter to screen for SSR primers. The research materials for primer verification and genetic relationship analysis were 106 eggplant germplasm samples (see Table 1), all from the Shanghai Sub-Center for Plant Variety Testing of the Ministry of Agriculture ...

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Abstract

The invention discloses screening and application of solanum melongene SSR (Simple Sequence Repeats) molecular marker core primers. A solanum melongene SSR molecular marker core primer group comprises17 SSR molecular marker core primer pairs. The stable and reliable solanum melongene SSR primers with high polymorphism are screened by extracting DNA and SSR-PCR of solanum melongene core germplasmswith great property differences. The screened 17 SSR primer pairs are used for analyzing 106 parts of solanum melongene culture variety resources; through similarity calculation and clustering analysis, the result shows that the screened 17 pairs of primers can be used for accurately and efficiently identifying the solanum melongene variety; and the foundation is laid for the application of an SSR molecular marker technology to solanum melongene germplasm genetic relationship analysis and variety identification.

Description

technical field [0001] The invention belongs to the technical field of molecular marker technology development and application, in particular to screening and application of a core primer for eggplant SSR molecular markers. Background technique [0002] Eggplant (Solanum melongene L.) is a crop of the Solanaceae (Solanaceae) genus Solanum (Solanum L.), the number of chromosomes is 2n=2x=24, its fruit is a berry, and it likes high temperature. Eggplant is known as cheese crisp and Kunlun melon in ancient times, commonly known as Diaocaizi, dwarf melon, and Luosu. It is an annual herb, and it is a perennial shrub in the tropics. Eggplant originates from the southern tropical region of Asia, and ancient India is its earliest domestication place. China has a long history of cultivating eggplant and is the second origin of eggplant. [0003] Eggplant is an important vegetable in our country and an important vegetable in Asia and the Mediterranean region. According to FAO data, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11C12Q1/6806
CPCC12Q1/6895C12Q1/6806C12Q2600/13C12Q2600/156C12Q2531/113
Inventor 邓姗任丽陈海荣章毅颖褚云霞赵洪李寿国
Owner SHANGHAI ACAD OF AGRI SCI
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