Set of SNP sites suitable for identifying variety and purity of hops and applications of set of SNP sites

A hops and locus technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of economic loss of beer enterprises, lack of simple and effective identification methods for hops raw materials, etc., and achieve accurate results, The effect of low cost and accurate detection results

Inactive Publication Date: 2017-12-08
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Abstract
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Problems solved by technology

In recent years, the procurement of hop raw materials by my country's beer production enterprises not only comes from real estate, but also imported hops also occupy a certain market share. However, some hop r

Method used

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  • Set of SNP sites suitable for identifying variety and purity of hops and applications of set of SNP sites
  • Set of SNP sites suitable for identifying variety and purity of hops and applications of set of SNP sites
  • Set of SNP sites suitable for identifying variety and purity of hops and applications of set of SNP sites

Examples

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Embodiment Construction

[0048] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0049] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0050] 1. Genomic DNA extraction from hop samples

[0051] Take a batch of 48 samples of Cascade hop granules collected on the market, use liquid nitrogen to grind and pulverize them several times, add them to a 2ml centrifuge tube, and use the Qiagen DNeasy Plant Mini Kit (250) plant DNA extraction kit to follow the steps Extract genomic DNA.

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Abstract

The invention discloses a set of SNP sites suitable for identifying the variety and purity of hops. The set of SNP sites comprises five SNP sites for hops totally. Based on a whole genome sequencing result for 10 representative hop varieties at home and abroad, comparison and screening are carried out on the SNP sites of the whole genome, at present, the identification for the variety and purity of hops of 5 SNP sites with relatively good polymorphism is verified, the 5 SNP sites can identify the variety of the hops, meanwhile, a genotype result is stable, and after the follow-up verification work for the authenticity of the SNP sites is completed, an SNP site fingerprint database of the hops is complemented to be complete. With the SNP sites and the identification method provided by the invention, an identification system for the variety and the purity of hops, which realizes low cost, high efficiency and standardization is provided for the beer production industry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a set of SNP sites suitable for identifying hop varieties and purity and applications thereof. Background technique [0002] Hops (Humulus lupulus), also known as hops, fragrant hops, hops and wild hops. In my country, it is mainly distributed in Xinjiang, Northeast China, North China and Shandong, Gansu and Shaanxi provinces. Hops is a crop with valuable economic value and medicinal value. Hops contain resins, volatile oils, flavonoids, tannins, choline, crude fibers, amino acids and other chemical components, which have antibacterial, antitumor, antioxidant, sedative and other activities. The resin contained in it gives the beer a unique aroma and refreshing bitter taste; tannin can not only eliminate the lactic acid bacteria produced during the fermentation process, but also solidify and separate the excess protein in the wine to clarify the wine. Therefore, hops ar...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 栾春光郝建秦刘诗宇王德良
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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