Floral character associated molecular marker screening method of amenone form chrysanthemum and application of method

Abstract

The invention belongs to the biotechnical field and provides a floral character QTL (quantitative trait locus) molecular marker screening method of amenone form chrysanthemum. The method can be used for positioning and cloning excellent genes of floral characters of amenone form chrysanthemum and cultivating novel varieties of amenone form chrysanthemum. The method comprises the following steps: I, obtaining a test material and phenome data; II, constructing a linkage map of chrysanthemum; III, combining phenome data with a molecular genetic map for QTL analysis of floral characters of amenone form chrysanthemum; and IV, determining the floral character associated molecular marker of the amenone form chrysanthemum. By using 160 F1 segregation population which is obtained by taking an amenone form chrysanthemum variety QX-053 as a female parent and a non-menone form chrysanthemum variety Nannongjingyan as a male parent, a plurality of molecular markers which are remarkably associated with floral characters of amenone form chrysanthemum. The molecular markers which are associated with floral characters of amenone form chrysanthemum are obtained for fine-mapping and cloning excellent genes of floral characters of amenone form chrysanthemum so as to greatly improve the selection efficiency, so that the amenone form chrysanthemum cultivating process is accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a QTL molecular marker screening method for flower organ characteristics of Tuogui-type chrysanthemums. The method can be used for positioning and cloning of excellent genes for flower organs of Tuogui-type chrysanthemums and cultivation of new varieties of Tuogui-type chrysanthemums. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium) originates in my country and is one of my country's top ten traditional famous flowers and the world's four major cut flowers. Chrysanthemum is extremely rich in flower type, flower color, and plant type. It is an important flower species used in potted plants, cut flowers, and garden covers. It has high ornamental and application value, and occupies a very important position in flower production. Chrysanthemums can be divided into small chrysanthemums and large chrysanthemums according to the diameter of the inflorescence, and small c...

AI Technical Summary

Problems solved by technology

At present, there are also reports on the molecular marker loci related to the ornamental traits of chrysanthemums, but there is no report on the gene mapping of flower organ traits of Tuogui type chrysanthemums at home and abroad.

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