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Mushroom bacterial standard detecting method

A standard detection and strain technology, applied in biochemical equipment and methods, measuring devices, and microbial determination/inspection, etc., can solve the problems of single identification, difficulty, and difficulty in distinguishing strains

Inactive Publication Date: 2008-03-05
上海市农业科学院食用菌研究所
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Problems solved by technology

According to the results of the current research, although there are certain differences among the mushroom strains, it is difficult to distinguish each strain through a single method. It is necessary to combine various methods to establish information files, so as to formulate corresponding strains. Standard Test Method
At present, there is no standard detection method for shiitake mushroom strains in China. The identification of new strains generally uses a few indicators, such as: the difference in agronomic status from the control strains (the size of the fruiting body is different, etc.), However, when the detection sample population is large, it is difficult to identify each strain by a single method

Method used

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Embodiment

[0518] 1. Collection of mushroom strains

[0519] The mushroom strains selected and bred by the breeding unit are collected from the main mushroom breeding scientific research units in my country. Each strain is accompanied by a certificate of the breeding unit, which proves that the strain is selected and named by the breeding unit itself. The total collection of mushroom standards There are 24 strains. In order to make the test data more reliable, 7 mushroom strains were added as reference during the test, a total of 31 strains, see Table 1 for details.

[0520] Table 1 24 standard test strains and reference strains of Lentinus edodes

[0521] Numbering

[0522] 5

[0523] 2. Research on biological characteristics

[0524] 2.1 Test material

[0525] 24 standard strains of mushroom production and 7 reference strains (Table 1).

[0526] 2.2 Test method

[0527] 2.2.1 Determination of mycelium growth rate: Inoculate mushroom mycelium blocks with a dia...

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Abstract

The present invention discloses standard Lentinus edodes seed detecting method, which includes 5 tested aspects including morphological characteristic, agronomic character, physiological character, quality character and molecular fingerprint of special DNA segment, and 36 test indexes, including 34 essential ones and 2 optional ones. Of the 34 essential indexes, there are 10 hypha character indexes and 24 cultivating character indexes.

Description

technical field [0001] The invention belongs to the field of strain detection, and in particular relates to a standard detection method for mushroom strains. Background technique [0002] The output of shiitake mushrooms in my country has grown rapidly from 19,500 tons in 1983 to 2.42 million tons in 2005, accounting for more than 70% of the total output of shiitake mushrooms in the world. Some experts predict that due to the rapid development of China's shiitake mushroom industry, it will become the edible fungus with the highest output in the world in the past 10 years. Chinese shiitake mushrooms have attracted the attention of people in the world's mushroom industry for their astonishing development speed, high-quality quality and low cost. Chinese shiitake mushrooms have become popular all over the world. [0003] The contribution rate of high-quality strains to the yield and quality of shiitake mushrooms is very important, which determines the important position of shii...

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/68G01N27/447
Inventor 谭琦郭力刚陈海荣王南曹晖吕波崔野韩堵苑苑顾晓君汪虹
Owner 上海市农业科学院食用菌研究所
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