Nanoparticles of cadmium alginate, lead alginate and copper alginate, and preparation method thereof, and applications of nanoparticles of cadmium alginate, lead alginate and copper alginate in preparation of electrochemical immunoassay probes

A technology of lead alginate and copper alginate, applied in the field of immunoassay and detection, to achieve good reproducibility, low detection limit and wide detection range

Inactive Publication Date: 2016-04-20
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The alginic acid microspheres prepared by these methods are all at the micron level. At present, the preparation of alginic acid microspheres with uniform particle size, good sphericity, and nano-scale is still a difficult problem to be solved.

Method used

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  • Nanoparticles of cadmium alginate, lead alginate and copper alginate, and preparation method thereof, and applications of nanoparticles of cadmium alginate, lead alginate and copper alginate in preparation of electrochemical immunoassay probes
  • Nanoparticles of cadmium alginate, lead alginate and copper alginate, and preparation method thereof, and applications of nanoparticles of cadmium alginate, lead alginate and copper alginate in preparation of electrochemical immunoassay probes
  • Nanoparticles of cadmium alginate, lead alginate and copper alginate, and preparation method thereof, and applications of nanoparticles of cadmium alginate, lead alginate and copper alginate in preparation of electrochemical immunoassay probes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of cadmium alginate, lead alginate or copper alginate nanospheres

[0039] (1) 2.0 g of triton, 1.0 g of n-hexanol, 3.0 g of n-octane, and 1.5 mL of 1.5% (w / w) sodium alginate aqueous solution were mixed and stirred for 45 min to prepare a uniform microemulsion 1 .

[0040] (2) Mix 2.0g triton, 1.0g n-hexanol, 3.0g n-octane, and 0.5M1.5mL of CdCl 2 Aqueous solution, Pb(NO 3 ) 2 Aqueous solution or CuCl 2 The aqueous solution was mixed and stirred for 45 minutes to prepare a uniform microemulsion 2.

[0041] (3) Add microemulsion 1 dropwise into microemulsion 2, stir and react at room temperature for 4 hours, centrifuge the product, and wash the precipitate with deionized water to obtain cadmium alginate, lead alginate or copper alginate nanospheres; transmission electron microscope The observed morphology of cadmium alginate, lead alginate and copper alginate nanospheres is as follows: figure 1 A, 1B and 1C are shown. The morphology of cadmium algin...

Embodiment 2

[0057] 1. Preparation of cadmium alginate, lead alginate or copper alginate nanospheres

[0058] (1) 2.5 g of triton, 1.5 g of n-hexanol, 3.5 g of n-octane, and 2.0 mL of 1.5% (w / w) sodium alginate aqueous solution were mixed and stirred for 45 min to prepare a uniform microemulsion 1 .

[0059] (2) Mix 2.5g triton, 1.5g n-hexanol, 3.5g n-octane, and 1.0M2.0mL of CdCl 2 Aqueous solution, Pb(NO 3 ) 2 Aqueous solution or CuCl 2 The aqueous solution was mixed and stirred for 1 hour to prepare a uniform microemulsion 2.

[0060] (3) Add microemulsion 1 dropwise into microemulsion 2, stir and react at room temperature for 5 hours, centrifuge the product, and wash the precipitate with deionized water to obtain cadmium alginate, lead alginate or copper alginate nanospheres.

[0061] 2. Preparation of immune probes and examples of their immune properties using cadmium alginate, lead alginate and copper alginate nanospheres:

[0062] 2.1 Tested sample: human serum

[0063] 2.2 Me...

Embodiment 3

[0076] 1. Preparation of cadmium alginate, lead alginate or copper alginate nanospheres

[0077] (1) 1.5 g of triton, 0.5 g of n-hexanol, 2.5 g of n-octane, and 1.0 mL of 1.5% sodium alginate aqueous solution were mixed and stirred for 45 min to prepare a uniform microemulsion 1 .

[0078] (2) Mix 1.5g triton, 0.5g n-hexanol, 2.5g n-octane, and 0.75M1.0mL of CdCl 2 aqueous solution, or Pb(NO 3 ) 2 Aqueous solution or CuCl 2 The aqueous solution was mixed and stirred for 30 minutes to prepare a uniform microemulsion 2.

[0079] (3) Add microemulsion 1 dropwise into microemulsion 2, stir and react at room temperature for 3 hours, centrifuge the product, and wash the precipitate with deionized water to obtain cadmium alginate, lead alginate and copper alginate nanospheres.

[0080]2. Preparation of immune probes and examples of their immune properties using cadmium alginate, lead alginate and copper alginate nanospheres:

[0081] 2.1 Tested sample: human serum

[0082] 2.2 ...

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Abstract

The present invention discloses nanoparticles of cadmium alginate, lead alginate and copper alginate, and a preparation method thereof, and applications of the nanoparticles of the cadmium alginate, the lead alginate and the copper alginate in preparation of electrochemical immunoassay probes. According to the present invention, cadmium ions, lead ions or copper ions are respectively subjected to cross-linking with sodium alginate to prepare the nano-scale nanoparticles of the cadmium alginate, the lead alginate and the copper alginate and generate distinguished electrochemical signals; the preparation method is characterized in that the preparation conditions are mild, and the product can be directly used for labeling without additional signal substances; with the electrochemical immunosensor prepared by using the nanoparticles of the cadmium alginate, the lead alginate and the copper alginate to label different antibodies, the simultaneous detection on three targets without partition can be achieved; and the nanoparticle immunoassay probes of the cadmium alginate, the lead alginate and the copper alginate, and the electrochemical immunosensor prepared from the nanoparticles of the cadmium alginate, the lead alginate and the copper alginate have wide application prospects in the immune sensing field.

Description

technical field [0001] The invention relates to a preparation method of cadmium alginate, lead alginate and copper alginate nanoparticles and their application in preparing immune probes, belonging to the field of electrochemical sensors, which can be used for immune analysis and detection. Background technique [0002] Cancer is a general term for a class of malignant tumors. In my country, 3 million people are diagnosed with cancer every year, and this number is still increasing year by year. Cancer treatment is difficult and has a high mortality rate, which is one of the problems that plague human beings. Studies have shown that early detection and early treatment can effectively improve the cure rate of cancer and reduce mortality. Early screening of cancer is mainly aimed at tumor markers. Tumor markers are usually directly produced by embryonic tissue and tumor tissue, and its concentration changes can reflect the dynamic changes of the tumor, and are related to tum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/14C08L5/04G01N33/543G01N33/574
Inventor 马占芳王子凤
Owner CAPITAL NORMAL UNIVERSITY
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