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Method for detecting diarrheic shellfish poison

A technology for diarrheal shellfish poisoning and shellfish, which is applied in the field of capillary electrophoresis electrochemical immunoassay for detecting diarrheal shellfish poisoning in shellfish samples, can solve problems such as unreported, and achieves low reagent consumption, high selectivity and high efficiency. Specific, high-sensitivity effects

Inactive Publication Date: 2010-09-08
QINGDAO UNIV OF SCI & TECH
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Problems solved by technology

But so far, the device and detection method for detecting diarrheal shellfish poisoning based on capillary electrophoresis electrochemical enzyme-linked immunoassay have not been reported.

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  • Method for detecting diarrheic shellfish poison
  • Method for detecting diarrheic shellfish poison
  • Method for detecting diarrheic shellfish poison

Examples

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Embodiment Construction

[0028] The following are specific examples for implementing the present invention, which serve to further clarify the content of the present invention and make it easier for readers to understand, but do not constitute a limitation or limitation on the scope of protection claimed by the present invention.

[0029] Experimental conditions:

[0030] MPI-A capillary electrophoresis numerical control high-voltage power supply, MPI-A capillary electrophoresis electrochemical analyzer (Xi'an Ruimai Analytical Instrument Co., Ltd.), three-electrode system: platinum disk working electrode, platinum wire auxiliary electrode and silver / silver chloride reference Specific electrode, PHS-3D pH meter (Shanghai Leici Scientific Instrument Co., Ltd.). o-aminophenol, phosphoric acid, boric acid, acetic acid, sodium hydroxide, horseradish peroxidase (Shanghai Xueman Biotechnology Co., Ltd.), diarrheal shellfish poisoning diagnostic kit (USA, Abraxis company), other reagents were of analytical g...

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Abstract

The invention discloses a novel capillary electrophoresis electrochemical immunoassay for determining a diarrheic shellfish poison in a shellfish sample. After the diarrheic shellfish poison in the shellfish sample and an enzyme labeled diarrheic shellfish poison competitively react with limited antibodies, the diarrheic shellfish poison and the enzyme labeled diarrheic shellfish poison are separated in a capillary tube. The separated enzyme labeled diarrheic shellfish poison and the enzyme labeled diarrheic shellfish poison-antibody composite respectively react with substrates in reaction tubes and enter an electrochemical detection cell for end-column electrochemical detection. The content of diarrheic shellfish poison in the shellfish sample is calculated according to the drop of the peak of the enzyme labeled diarrheic shellfish poison-antibody composite. The invention has the advantages that the sample processing processes are simplified, the selectivity is good, the accuracy is high and the method is the ideal method for detecting the diarrheic shellfish poison in the shellfish sample.

Description

technical field [0001] The invention relates to capillary electrophoresis technology, in particular to a capillary electrophoresis electrochemical immunoassay method for detecting diarrheal shellfish poison in shellfish samples. Background technique [0002] In recent years, the ever-increasing pollution of the marine environment has led to an increase in the number and scale of harmful red tide outbreaks. A large number of red tide toxins or shellfish poisons produced by red tide organisms not only seriously damage marine fishery resources and aquaculture, and deteriorate the marine environment, but also can go up through the food chain layer accumulation, endangering human health. [0003] Diarrheal shellfish poisoning mainly comes from organisms of the genus Dinosaurs and Prorocentrum, including at least 15 compounds, 13 of which have been identified. Diarrheal shellfish poisoning is a fatty acid derivative with multiple cyclic ethers, which is an unsaturated hydrocarbon...

Claims

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Application Information

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IPC IPC(8): G01N33/561G01N33/53G01N27/447
Inventor 张召香李晓琳葛安青李雪梅梅振华张书圣
Owner QINGDAO UNIV OF SCI & TECH
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