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Rotary type electrochemical immunoassay paper chip analysis device and detection method thereof

An analysis device and paper chip technology, which is applied in measurement devices, analytical materials, scientific instruments, etc., can solve problems such as limiting the application of paper chips, and achieve the effects of extensive on-site real-time detection, simple operation, and high-sensitivity quantitative analysis.

Active Publication Date: 2018-05-04
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The flow of the fluid on the paper chip is mainly driven by capillary force without the need for external power. However, this advantage brings certain difficulties to the manipulation of the fluid in the analysis of the paper chip, which limits the multi-step and multi-component analysis of the paper chip. Applications in complex systems

Method used

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  • Rotary type electrochemical immunoassay paper chip analysis device and detection method thereof
  • Rotary type electrochemical immunoassay paper chip analysis device and detection method thereof
  • Rotary type electrochemical immunoassay paper chip analysis device and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Preparation of a Rotary Electrochemical Immuno-Paper Chip Analysis Device

[0027] Such as figure 1 As shown, the rotating paper chip analysis device is composed of three layers of paper chips, the auxiliary disk C, the detection disk B and the washing disk A, which are rotationally connected by rivets. The detection disk B has four immune regions 5, wherein the working electrodes 4 are printed on the immune regions on one side of the disk. Four circular holes 2 and a circular hydrophilic area printed with a reference electrode 6 and a counter electrode 7 are distributed on the auxiliary disk C. The detection disk B and the auxiliary disk C constitute the upper valve of the device. When the auxiliary disk B is rotated to disconnect the detection channel, the immune area 5 on the detection disk is exposed and the reaction reagent is added to the immune reaction. On the other hand, the rotation of the auxiliary disk B The detection channel can also be communi...

Embodiment 2

[0029] Example 2: Operation process of immunoassay on the device

[0030] In the rotating paper chip analysis device obtained in Example 1 above, each paper chip disk can rotate around the center, and the connection and disconnection of the fluid channels on the paper chip can be controlled by rotating the auxiliary disk and the washing disk, so as to realize multi-step immune analysis process.

[0031] The immunoassay process controlled by the rotary valve is as follows: For the reaction process in the immunoassay, firstly, the auxiliary disc C is rotated to close the detection channel, so that the immune area 5 of the detection disc B is revealed, and then the washing disc A is rotated to clean the washing channel on the washing disc. 1 is disconnected from the immune area 5 on the detection disc, at this time, the reaction reagent can be added to the immune area 5 for reaction. After the reaction is completed, the immune area needs to be washed to remove unreacted reagents...

Embodiment 3

[0034] Example 3: Quantitative determination of tumor markers by the device

[0035] The detection of tumor markers using the above-mentioned device:

[0036] 1) The capture antibody is immobilized on the modified multi-walled carbon nanotubes and the chitosan-loaded working area through the cross-linking reaction of glutaraldehyde. Specifically, first rotate the auxiliary plate and the washing plate to expose the immune area on the detection plate, then add 5 μL of multi-walled carbon nanotubes to the immune area to improve the conductivity of the electrode, and then add 5 μL of 0. 25 mg / mL chitosan was added to the immune area and dried in the air; then 5 μL of 2.5% glutaraldehyde was added to activate the immune area for 2 h; the washing plate was rotated and the unreacted glutaraldehyde was removed by adding washing buffer Finally, CEA and PSA capture antibodies were added to the immune area, incubated at room temperature for 40 min, and then the washing plate was rotated...

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Abstract

The invention relates to the technical field of electrochemical analysis, biological immunity and micro-fluidic chips, in particular to a rotary type electrochemical immunoassay paper chip analysis device and a detection method thereof. The device comprises an auxiliary disc, a reagent disc and a washing disc which are rotationally connected, wherein the auxiliary disc, the reagent disc and the washing disc can independently rotate; a respective fluid passage is formed in each of the auxiliary disc, the reagent disc and the washing disc; holes are formed in each fluid passage; the fluid is controlled to flow in each hole when the holes formed in the auxiliary disc, the reagent disc and the washing disc and are rotated to the mutually communicated holes. Each layer of paper chips in the device can independently rotate around the center, so that a rotating valve is ingeniously integrated onto the paper chip device for controlling the flowing of fluid on the paper chip. The preparation ofthe device in the invention is simple and fast; the operation is convenient; a novel device is provided for the fast and convenient medical instant diagnosis.

Description

technical field [0001] The invention relates to the technical fields of electrochemical analysis, biological immunity and microfluidic chip, and more specifically relates to a rotary electrochemical immunological paper chip analysis device and a detection method thereof. Background technique [0002] As an important branch of microfluidics, microfluidic paper chips use paper as a supporting material, which has the advantages of low cost, easy fabrication, and portability. Since it was proposed by the Whiteside research group of Harvard University in 2007, it has rapidly developed into a An emerging analytics platform. Compared with conventional test strip detection, the microfluidic paper chip analysis device can realize multi-channel and multi-indicator parallel quantitative analysis, meeting the needs of fast, cheap and convenient analysis of disease diagnosis, food safety and environmental pollutants. Various detection methods are currently applied to paper chip devices ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/543
CPCG01N33/54373G01N33/57484
Inventor 李博伟孙献歌陈令新付龙文
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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