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Device and method for detecting paralytic shellfish poisoning toxin

A technology for paralytic and shellfish poisoning, which is applied in the field of capillary electrophoresis electrochemical immunoassay devices for detecting paralytic shellfish poisoning, can solve problems such as no reports, and achieves less reagent consumption, simplified operation steps, high selectivity and specialization. Oneness effect

Inactive Publication Date: 2011-08-17
QINGDAO UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the device and detection method for detecting paralytic shellfish poisoning based on capillary electrophoresis electrochemical enzyme-linked immunoassay have not been reported

Method used

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  • Device and method for detecting paralytic shellfish poisoning toxin
  • Device and method for detecting paralytic shellfish poisoning toxin
  • Device and method for detecting paralytic shellfish poisoning toxin

Examples

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Embodiment 1

[0028] Example 1 Construction of capillary electrophoresis electrochemical immunoassay device

[0029] A specific capillary electrophoresis electrochemical immunoassay device for detecting paralytic shellfish poisoning such as figure 2 shown. Place a capillary column (Hebei Yongnian Optical Fiber Factory, 20 cm long, 50 μm inner diameter, 375 μm outer diameter) between buffer solution pool 3 (0.8 cm diameter, 1.5 cm height) and substrate solution pool 5 (0.8 cm diameter, 1.5 cm height). ), the capillary column was cut a small hole 5cm from the end, and communicated with the substrate solution in 5. The inside of the cell body 5 is filled with substrate solution, inserted electrodes and plastic tubes. There is a horizontal through hole in the middle of both sides of the cell body, which is used to place capillary columns, so that a section of capillary tube protrudes from both ends of the cell body, and the two sections are respectively As separation capillary 1 and reaction...

Embodiment 2

[0032] Example 2 Detection of paralytic shellfish poisoning in shellfish samples using capillary electrophoresis electrochemical immunoassay method

[0033]Shellfish (scallops or oysters) samples were removed from the shell, and the shellfish was soaked in distilled water for 3 times, homogenized for 10 minutes, adjusted to pH 3.5-4.0 with 6mol / L hydrochloric acid, bathed in ice water for 15 minutes, ultrasonicated for 10 minutes, and refrigerated at 4°C for 10 minutes. Centrifuge for 10 minutes while cool, pour out the supernatant and filter with a 0.2 μm membrane filter;

[0034] Add excess enzyme-labeled PSP and a certain amount of antibody to the shellfish sample solution treated above, and incubate and react at 37°C for 30 minutes. The PSP in the shellfish sample competes with the enzyme-labeled antigen for a certain amount of antibody:

[0035] Paralytic shellfish poison+enzyme-labeled paralytic shellfish poison (excessive)+antibody (certain amount)=paralytic shellfish p...

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Abstract

The invention discloses a novel capillary electrophoresis electrochemical immunoassay device for measuring paralytic shellfish poisoning toxin and a related detection method. The device comprises a notched capillary and a substrate tank, wherein two sides of the tank body are horizontally provided with a through hole; the capillary is inserted into the through hole, and a section of capillary extends out of the two sides of the substrate tank respectively and is used as a separation tube and a reaction tube respectively; the inlet end of the reaction tube is connected with a buffer solution tank; a buffer hydraulic tank and a substrate hydraulic tank are connected above the buffer solution tank and the substrate tank respectively; and the tail end of the capillary is inserted into an electrochemical detection tank. The detection method comprises that: the paralytic shellfish poisoning toxin in a shellfish sample and the enzyme-labeled paralytic shellfish poisoning toxin undergo competitive reaction with limited antibodies and then are separated in the capillary, and the separated enzyme-labeled paralytic shellfish poisoning toxin and the paralytic shellfish poisoning toxin-antibody complex are reacted with the substrate in the reaction tube respectively and then enter the electrochemical detection tank for detection.

Description

technical field [0001] The invention relates to capillary electrophoresis technology, in particular to a capillary electrophoresis electrochemical immunoassay device and a detection method for detecting paralytic shellfish poisoning. Background technique [0002] In recent years, the ever-increasing pollution of the marine environment has led to an increase in the number and scale of harmful red tide outbreaks. A large number of red tide toxins or shellfish poisons produced by red tide organisms not only seriously damage marine fishery resources and aquaculture, and deteriorate the marine environment, but also can go up through the food chain layer accumulation, endangering human health. Paralytic shellfish poisoning is distributed in coastal areas all over the world, and the main components are different due to the different types of red tide organisms and their geographical distribution. Paralytic shellfish poisoning can kill a large number of fish, and can accumulate in ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N27/447
Inventor 梅振华张召香李雪梅任锐李晓琳葛安青张书圣
Owner QINGDAO UNIV OF SCI & TECH
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