Buffer solution for electrochemiluminescence immunity analyzer and preparation method of buffer solution

A technology of luminescence immunity and buffer solution, which is applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, analysis of materials, etc., can solve the problems of dependence on imports of buffer solution, high amount of tripropylamine, and high cost of reagents. Low production cost, reduced hazard, simple formulation effect

Active Publication Date: 2015-06-10
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the current situation that the buffer solution used in the electrochemiluminescence immunoassay analyzer in the prior art relies on imports for a long time, the cost of the reagent is high, and the defect of a large amount of tripropylamine is used, the purpose of the present invention is to provide a low-cost, small amount of tripropylamine, and superior reagent performance. Buffer solution for electrochemiluminescence immunoassay analyzer and preparation method thereof

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  • Buffer solution for electrochemiluminescence immunity analyzer and preparation method of buffer solution
  • Buffer solution for electrochemiluminescence immunity analyzer and preparation method of buffer solution
  • Buffer solution for electrochemiluminescence immunity analyzer and preparation method of buffer solution

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Embodiment 1

[0031] Embodiment 1, Buffer solution for electrochemiluminescence immunoassay analyzer of the present invention

[0032] Potassium dihydrogen phosphate 40.0g / L

[0033] Tween 20 0.8g / L

[0034] Lauryl polyethylene glycol ether 0.1g / L

[0035] Polydocanol 1.0g / L

[0036] Tripropylamine 12.0g / L

[0037] Citric acid 0.5g / L

[0038] The pH of the buffer is 6.5.

[0039] Weigh the required potassium dihydrogen phosphate, citric acid, polydocanol, Tween 20 and lauryl polyethylene glycol ether according to the formula and put them into a beaker, add an appropriate amount of deionized water, heat and stir until completely dissolved, After cooling, add tripropylamine and stir for 20 min. After stirring evenly, dilute to 1 L with deionized water, adjust the pH value to 7.0 with NaOH solution, and filter to obtain a buffer solution.

Embodiment 2

[0040] Embodiment 2, Buffer solution for electrochemiluminescence immunoassay analyzer of the present invention

[0041] Potassium dihydrogen phosphate 46.8 g / L

[0042] Tween 20 1.0g / L

[0043] Lauryl polyethylene glycol ether 0.5g / L

[0044] Polydocanol 1.009g / L

[0045] Tripropylamine 15g / L

[0046] Citric acid 0.6085g / L

[0047] The pH value of the buffer is 6.8.

[0048] Weigh the required potassium dihydrogen phosphate, citric acid, polydocanol, Tween 20 and lauryl polyethylene glycol ether according to the formula and put them into a beaker, add an appropriate amount of deionized water, heat and stir until completely dissolved, After cooling, add tripropylamine and stir for 20 min. After stirring evenly, dilute to 1 L with deionized water, adjust the pH value to 6.8 with NaOH solution, and filter to obtain a buffer solution.

Embodiment 3

[0049] Embodiment 3, Buffer solution for electrochemiluminescence immunoassay analyzer of the present invention

[0050] Potassium dihydrogen phosphate 48.0 g / L

[0051] Tween 20 3.0g / L

[0052] Lauryl polyethylene glycol ether 0.6g / L

[0053] Polydocanol 5.0g / L

[0054] Tripropylamine 16.0g / L

[0055] Citric acid 1.0 g / L

[0056] The pH of the buffer is 7.0.

[0057] Weigh the required potassium dihydrogen phosphate, citric acid, polydocanol, Tween 20 and lauryl polyethylene glycol ether according to the formula and put them into a beaker, add an appropriate amount of deionized water, heat and stir until completely dissolved, After cooling, add tripropylamine and stir for 20 minutes. After stirring evenly, dilute to 1 L with deionized water, adjust the pH value to 0.0 with NaOH solution, and filter to obtain a buffer solution.

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Abstract

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to a buffer solution for an electrochemiluminescence immunity analyzer and a preparation method of the buffer solution. The buffer solution for the electrochemiluminescence immunity analyzer comprises the following components: potassium dihydrogen phosphate 4, tween-20, dodecyl polyglycol ether, polidocanol, tripropyl amine and citric acid; and the pH value of the buffer solution is 6.5-7.0. The buffer solution for the electrochemiluminescence immunity analyzer is simple to prepare and less in usage amount of tripropyl amine to reduce harms of tripropyl amine to bodies of operators and reduce the burden of the environment; the R value of a correlation coefficient after linear regression reaches more than 0.99 compared with an imported buffer solution for an electrochemical immunity analyzer and expresses relatively good relativity; and moreover, the buffer solution is low in production cost, and the selling price can be reduced by 30% compared with a reagent for an equivalent blood cell analyzer on the market, so that the buffer solution for the electrochemiluminescence immunity analyzer is beneficial for large-scale popularization and application.

Description

technical field [0001] The invention belongs to reagents for medical diagnostic instruments, in particular to a buffer solution for electrochemiluminescence immunoassay analyzers and a preparation method thereof. Background technique [0002] At present, the commonly used methods for the quantitative detection of disease-related trace substances in the human body include chemiluminescence immunoassay and electrochemiluminescence immunoassay. The precision is high, and the reagent is stable, and it can be analyzed fully automatically, so it is an ideal marker immunoassay method. [0003] However, the electrochemiluminescence immunoassay is known for its high sensitivity (up to 10 -11 ~10 -17 mol / L) and good repeatability have been widely used. Electrochemiluminescence immunoassays are divided into luminescent systems of organic compounds and luminescent systems of inorganic substances. Organic luminescent substances include hydrazide compounds, acridinium esters and lucig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N21/76G01N33/574G01N33/78G01N33/74
CPCG01N21/76G01N33/531G01N33/57438G01N33/57473G01N33/74G01N33/78G01N2800/36G01N2800/7028
Inventor 何林辉李冰黄伟豪
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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