Buffer solution for electrochemiluminescence immunity analyzer and preparation method of buffer solution
A technology of luminescence immunity and buffer solution, which is applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, analysis of materials, etc., can solve the problems of dependence on imports of buffer solution, high amount of tripropylamine, and high cost of reagents. Low production cost, reduced hazard, simple formulation effect
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Embodiment 1
[0031] Embodiment 1, Buffer solution for electrochemiluminescence immunoassay analyzer of the present invention
[0032] Potassium dihydrogen phosphate 40.0g / L
[0033] Tween 20 0.8g / L
[0034] Lauryl polyethylene glycol ether 0.1g / L
[0035] Polydocanol 1.0g / L
[0036] Tripropylamine 12.0g / L
[0037] Citric acid 0.5g / L
[0038] The pH of the buffer is 6.5.
[0039] Weigh the required potassium dihydrogen phosphate, citric acid, polydocanol, Tween 20 and lauryl polyethylene glycol ether according to the formula and put them into a beaker, add an appropriate amount of deionized water, heat and stir until completely dissolved, After cooling, add tripropylamine and stir for 20 min. After stirring evenly, dilute to 1 L with deionized water, adjust the pH value to 7.0 with NaOH solution, and filter to obtain a buffer solution.
Embodiment 2
[0040] Embodiment 2, Buffer solution for electrochemiluminescence immunoassay analyzer of the present invention
[0041] Potassium dihydrogen phosphate 46.8 g / L
[0042] Tween 20 1.0g / L
[0043] Lauryl polyethylene glycol ether 0.5g / L
[0044] Polydocanol 1.009g / L
[0045] Tripropylamine 15g / L
[0046] Citric acid 0.6085g / L
[0047] The pH value of the buffer is 6.8.
[0048] Weigh the required potassium dihydrogen phosphate, citric acid, polydocanol, Tween 20 and lauryl polyethylene glycol ether according to the formula and put them into a beaker, add an appropriate amount of deionized water, heat and stir until completely dissolved, After cooling, add tripropylamine and stir for 20 min. After stirring evenly, dilute to 1 L with deionized water, adjust the pH value to 6.8 with NaOH solution, and filter to obtain a buffer solution.
Embodiment 3
[0049] Embodiment 3, Buffer solution for electrochemiluminescence immunoassay analyzer of the present invention
[0050] Potassium dihydrogen phosphate 48.0 g / L
[0051] Tween 20 3.0g / L
[0052] Lauryl polyethylene glycol ether 0.6g / L
[0053] Polydocanol 5.0g / L
[0054] Tripropylamine 16.0g / L
[0055] Citric acid 1.0 g / L
[0056] The pH of the buffer is 7.0.
[0057] Weigh the required potassium dihydrogen phosphate, citric acid, polydocanol, Tween 20 and lauryl polyethylene glycol ether according to the formula and put them into a beaker, add an appropriate amount of deionized water, heat and stir until completely dissolved, After cooling, add tripropylamine and stir for 20 minutes. After stirring evenly, dilute to 1 L with deionized water, adjust the pH value to 0.0 with NaOH solution, and filter to obtain a buffer solution.
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