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A kind of electrochemical immunoassay method for the detection of bisphenol A

An immune analysis and electrochemical technology, applied in the direction of material electrochemical variables, biological testing, material inspection products, etc., can solve the problems of difficult real-time on-site detection, long detection time, expensive instruments, etc., and achieve easy access and few reaction steps , cheap effect

Inactive Publication Date: 2018-05-29
TIANJIN UNIV OF SCI & TECH +3
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Problems solved by technology

[0005] In view of this, the present invention aims to propose an electrochemical immunoassay method for the detection of bisphenol A, to overcome the problems of traditional instrument detection and enzyme-linked immunoassay method for long detection time, expensive instruments, and difficulty in real-time on-site detection.

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  • A kind of electrochemical immunoassay method for the detection of bisphenol A

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Embodiment 1

[0030] Synthesis of Bisphenol A

[0031] Weigh 0.5-2.0g of bisphenol A and 0.5-2.5g of potassium carbonate and dissolve in 5-15mL of anhydrous dimethylformamide, and add 0.5-1.5g of ethyl 4-bromobutyrate. The reaction system was stirred at 65-80° C. for 8-16 hours. Then the reaction mixture was extracted with ethyl acetate, dried over sodium sulfate, and spin-dried under reduced pressure. The resultant is separated and purified by a silica gel column using a mixture of methanol and dichloromethane with a volume ratio of 1 / 198 to 2 / 98 as a developing solvent to obtain bisphenol A intermediate product a1. Dissolve 1.0~2.5gal in 3~7mL of methanol, and then add it into 1~5mL of 3~5mol / L sodium hydroxide aqueous solution. Stir at 20-30°C for 8-16 hours. After the reaction mixture was washed with ethyl acetate, the aqueous phase was acidified to pH 2-4 with 28-35% HCl. The acidified aqueous phase was then extracted with ethyl acetate. Next, the organic phases were combined, dri...

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Abstract

The invention provides an electrochemical immunoassay method for bisphenol A detection. The method comprises the steps of 1, competing reaction, wherein 5-20 microlitres of iso-volume mixed gradient dilution type solution of a sample to be detected or sample extraction solution and enzyme-labelled antigen solution diluted by phosphate buffered solution are added onto the surface of an enclosed electrode dropwise, the enclosed electrode is placed at 20-35 DEG C for incubation lasting 0.5-1.5 h, and flushing and blow-drying are conducted; 2, reduction peak current value detection, wherein the flushed electrode obtained from step 1 is immersed in 2-6 millilitre of ferrocenylmethanol solution of 0.2-1.5 mM, 20-60 microlitres of hydrogen peroxide of 5-15 mM is added at the same time, and then reduction peak current value is detected with the cyclic voltammetry method with a saturated calomel electrode as the reference electrode and a platinum electrode as the counter electrode. The immunoassay method is used for detection. The method overcomes the defect of a traditional enzyme-linked immunoassay method that detection is time-consuming, and reduces detection time.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and residue analysis of small molecule compounds, and in particular relates to an electrochemical immunoassay method for bisphenol A detection. Background technique [0002] Bisphenol-A is an endocrine disruptor derived from industrial chemicals. As an important organic chemical raw material, bisphenol A is mainly used in the production of various polymer materials such as polycarbonate and epoxy resin, and fine chemical products such as plasticizers, flame retardants, and coatings. From mineral water bottles, medical equipment to food packaging, most of them contain bisphenol A. However, because the surface of its finished product is easily degraded under the action of strong detergents, acidic or high-temperature liquids, there are residues in environmental water bodies, drinking water, aquatic products, vegetables, fruits and canned foods. Toxicology experiments have shown that bisphe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N27/48
CPCG01N27/48G01N33/53
Inventor 陆旸丁敏玲李梦娟张燕刘国珍李翔王硕
Owner TIANJIN UNIV OF SCI & TECH
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