Assembly array type multi-channel capacitance immune sensor based on glass capillary structure

An immunosensor, glass capillary technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of complex detection process and many mutual interference factors, and achieve the effects of simple equipment, low price, and easy automation

Inactive Publication Date: 2010-10-27
周亚民
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AI Technical Summary

Problems solved by technology

[0003] Immunological detection of multiple components of a sample generally requires a variety of differen

Method used

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Examples

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Embodiment 1

[0021] Example 1: Conductivity immunosensor for detecting IgG, IgM, IgA

[0022] (1) Structure of immune electrode: 10% aminopropyltriethoxysilane NH 2 (CH 2 ) 3 Si(OEt) 3 Inject the alcohol solution into the capillary (length 80mm, inner diameter 1mm), keep for 20 minutes, introduce amino groups on the surface of the capillary, and then inject 10% glutaraldehyde solution after cleaning, then inject 0.1% goat anti-human IgM reagent, keep for 20 minutes , Wash with 0.1M phosphate buffer solution, insert platinum wire to fix it as electrode.

[0023] (3) Inject 0.1% BSA solution, seal the active adsorption point, wash and dry.

[0024] (4) Assembly: Combine two glass capillaries with goat anti-human IgG fixed to obtain IgG immunoassay components. Similarly, the glass capillaries fixed with goat anti-human IgM are combined together to obtain IgM immunodetection components, and the glass capillaries fixed with goat anti-human IgA are combined together to obtain IgA immunodetection compo...

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Abstract

The invention belongs to the technical field of electrochemical immunoassay, and relates to an assembly array type multi-channel capacitance immune sensor based on a glass capillary structure, which is formed through the following steps: fixing antibody A (antigen A) on the inner wall of a glass capillary through aminosilane reagent and glutaraldehyde, inserting a platinum wire electrode into the glass capillary, fixing the platinum wire electrode in the glass capillary, injecting nonspecific protein, sealing active adsorption sites, washing, drying, and combining two glass capillaries formed through the above steps, thereby obtaining an immune electrode pair sensitive to A. Then an immune electrode pair sensitive to B, an immune electrode pair sensitive to C and an immune electrode pair sensitive to D are formed in sequence in the identical method. The assembly array type multi-channel capacitance immune sensor can be obtained through assembling the electrode pairs. A corresponding immune reaction substance is cultured and washed in the glass capillary and is then inserted in substrate solution with urinary enzyme, and the urinary enzyme labeled antigen (urinary enzyme labeled antibody) catalyzes the reaction of the substrate, thereby increasing the capacitance of the solution. Then the content of the antigen (antibody) can be measured.

Description

Technical field [0001] The invention relates to an electrochemical conductivity immunosensor array. Background technique [0002] The conductivity type immunosensor is a sensing device that uses the immune response to change the conductivity of the solution or film for analysis. Enzymes catalyze the reaction of the substrate, leading to changes in the type and concentration of ions in the reaction system, thereby changing the conductivity of the solution. For example, urease catalyzes urea to produce ion product NH 1 + When the NH formed 4 + The increase in the conductivity of the solution is related to the amount of enzyme, so the conductivity measurement can determine the amount of enzyme labeled tracer in enzyme-linked immunosorbent. The present invention uses this phenomenon to design an immune reaction in a glass capillary, introduces a urease marker, and through a competitive reaction, the enzyme marker is fixed on the inner surface of the capillary, and the conductance of...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N27/327
Inventor 周亚民
Owner 周亚民
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