Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing electrochemical immunosensing interface based on conductive gel with active sites

A conductive gel, active site technology, applied in the field of immunodetection or electrochemical sensing, can solve the problems of difficult molecular skeleton binding sites, destroy the Π conjugated network structure, hinder electron transport, etc., and achieve rapid and mild antibody chemistry. Fixed, broad application prospects, the effect of excellent electrical conductivity

Active Publication Date: 2021-11-09
TAIYUAN UNIV OF TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, conductive gels are usually inert, and it is very difficult to introduce binding sites on their molecular backbones.
Although copolymerization of conductive monomers with active site-containing units such as acrylic acid can easily introduce binding sites, non-conductive active units will destroy the continuous Π-conjugated network structure in the gel, hinder electron transport, and seriously weaken its conductivity.
Therefore, how to prepare a conductive gel electrochemical sensing interface with active sites and maintain excellent conductivity still faces great challenges.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing electrochemical immunosensing interface based on conductive gel with active sites
  • Method for constructing electrochemical immunosensing interface based on conductive gel with active sites
  • Method for constructing electrochemical immunosensing interface based on conductive gel with active sites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The present invention will now be described in further detail in conjunction with the accompanying drawings and the following examples, but it should be understood that these examples are for illustrative purposes only, and should not be construed as limitations on the implementation of the present invention.

[0022] In this example, the supramolecular complex of vinyl ferrocene and monoaldehyde cyclodextrin is used to copolymerize with pyrrole to obtain a conductive gel, which is loaded on the working electrode and the antibody is immobilized to obtain an electrochemical immunosensing interface. Specific steps are as follows:

[0023] (1) Preparation of supramolecular complexes

[0024] Weigh 2.33 g of β-cyclodextrin and 0.523 g of 2-iodoxybenzoic acid into a 100 mL Erlenmeyer flask, add 20 mL of dimethyl sulfoxide to it, ultrasonically dissolve it for 2 min, and react at room temperature for 24 h. After completion, it was settled with cooling acetone, and a white pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for constructing an electrochemical immunosensing interface by using a conductive gel with an active site, and belongs to the field of immunodetection or electrochemical sensing. The purpose of the present invention is to first use the host-guest recognition effect of vinyl ferrocene and monoaldehyde cyclodextrin to obtain a supramolecular complex, and then copolymerize into a conductive gel with controllable morphology by a mixed solvent method and load it on the working electrode Finally, the antibody was chemically immobilized by aldehyde-amine condensation reaction to obtain an electrochemical immunosensing interface. The preparation conditions of the present invention are mild, the reaction is fast, and the obtained interface not only has excellent conductivity, but also can realize a large amount of immobilization of antibodies, and finally can directly and sensitively recognize the antigen to be tested through the change of the current signal. Therefore, the electrochemical immunosensor provided by the present invention The sensing interface will have broad application prospects in the fields of immunoassay and electrochemical sensing.

Description

technical field [0001] The invention relates to a method for constructing an electrochemical immunosensing interface by using a conductive gel with an active site, and belongs to the field of immunodetection or electrochemical sensing. Background technique [0002] Electrochemical immunosensing is a combination of electrochemical analysis technology and immunodetection, and realizes the analysis of the analyte by using the specific recognition of antigens and antibodies and the change of electrical signals in electrochemistry. With its strong specificity and high sensitivity , good accuracy and easy operation, electrochemical immunosensing has shown great development prospects in the detection of cancer, heart disease and various metabolic diseases. How to improve its detection effect has always been one of the hot spots that researchers are most concerned about. Among them, since the recognition of the antibody and the antigen to be tested and the electron transfer between...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N33/531
CPCG01N27/26G01N27/30G01N33/531
Inventor 马姣毛晓慧丛虹霄张若茜
Owner TAIYUAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com