Method for constructing electrochemical immunosensing interface based on conductive gel with active sites

Abstract

The invention relates to a method for constructing an electrochemical immunosensing interface by using a conductive gel with an active site, and belongs to the field of immunodetection or electrochemical sensing. The purpose of the present invention is to first use the host-guest recognition effect of vinyl ferrocene and monoaldehyde cyclodextrin to obtain a supramolecular complex, and then copolymerize into a conductive gel with controllable morphology by a mixed solvent method and load it on the working electrode Finally, the antibody was chemically immobilized by aldehyde-amine condensation reaction to obtain an electrochemical immunosensing interface. The preparation conditions of the present invention are mild, the reaction is fast, and the obtained interface not only has excellent conductivity, but also can realize a large amount of immobilization of antibodies, and finally can directly and sensitively recognize the antigen to be tested through the change of the current signal. Therefore, the electrochemical immunosensor provided by the present invention The sensing interface will have broad application prospects in the fields of immunoassay and electrochemical sensing.

Description

technical field [0001] The invention relates to a method for constructing an electrochemical immunosensing interface by using a conductive gel with an active site, and belongs to the field of immunodetection or electrochemical sensing. Background technique [0002] Electrochemical immunosensing is a combination of electrochemical analysis technology and immunodetection, and realizes the analysis of the analyte by using the specific recognition of antigens and antibodies and the change of electrical signals in electrochemistry. With its strong specificity and high sensitivity , good accuracy and easy operation, electrochemical immunosensing has shown great development prospects in the detection of cancer, heart disease and various metabolic diseases. How to improve its detection effect has always been one of the hot spots that researchers are most concerned about. Among them, since the recognition of the antibody and the antigen to be tested and the electron transfer between...

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Problems solved by technology

[0004] However, conductive gels are usually inert, and it is very difficult to introduce binding sites on their molecular backbones.

Although copolymerization of conductive monomers with active site-containing units such as acrylic acid can easily introduce binding sites, non-conductive active units will destroy the continuous Π-conjugated network structure in the gel, hinder electron transport, and seriously weaken its conductivity.

Therefore, how to prepare a conductive gel electrochemical sensing interface with active sites and maintain excellent conductivity still faces great challenges.

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