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Method of constructing electrochemical immunosensing interface based on conductive gel with active sites

A conductive gel and active site technology, applied in the field of immunoassay or electrochemical sensing, can solve the problems of molecular skeleton binding sites, destroy Π conjugated network structure, hinder electron transport and other problems, and achieve rapid and gentle antibody chemistry. Fixed, broad application prospects, excellent conductivity effect

Active Publication Date: 2020-01-17
TAIYUAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, conductive gels are usually inert, and it is very difficult to introduce binding sites on their molecular backbones.
Although copolymerization of conductive monomers with active site-containing units such as acrylic acid can easily introduce binding sites, non-conductive active units will destroy the continuous Π-conjugated network structure in the gel, hinder electron transport, and seriously weaken its conductivity.
Therefore, how to prepare a conductive gel electrochemical sensing interface with active sites and maintain excellent conductivity still faces great challenges.

Method used

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  • Method of constructing electrochemical immunosensing interface based on conductive gel with active sites
  • Method of constructing electrochemical immunosensing interface based on conductive gel with active sites
  • Method of constructing electrochemical immunosensing interface based on conductive gel with active sites

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Embodiment Construction

[0021] The present invention will now be described in further detail in conjunction with the accompanying drawings and the following examples, but it should be understood that these examples are for illustrative purposes only, and should not be construed as limitations on the implementation of the present invention.

[0022] In this example, the supramolecular complex of vinyl ferrocene and monoaldehyde cyclodextrin is used to copolymerize with pyrrole to obtain a conductive gel, which is loaded on the working electrode and the antibody is immobilized to obtain an electrochemical immunosensing interface. Specific steps are as follows:

[0023] (1) Preparation of supramolecular complexes

[0024] Weigh 2.33 g of β-cyclodextrin and 0.523 g of 2-iodoxybenzoic acid in a 100 mL Erlenmeyer flask, add 20 mL of dimethyl sulfoxide to it, ultrasonically dissolve it for 2 min, react at room temperature for 24 h, and the reaction is complete After that, it was settled with cooling aceton...

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Abstract

The invention relates to a method of constructing an electrochemical immunosensing interface based on conductive gel with active sites and belongs to the field of immunoassay or electrochemical sensing. According to the purposes of the method, firstly, the host-guest recognition action of vinyl ferrocene and monoaldehyde cyclodextrin is utilized to obtain a supramolecular complex, then the shape-controlled conductive gel is synthesized through copolymerization by use of a mixed solvent method and is loaded to a working electrode, and finally the electrochemical immunosensing interface is obtained through an aldehyde-amine condensation reaction of chemically immobilized antibodies. According to the method, preparation conditions are mild, the reaction is rapid, the obtained interface has excellent conductivity, meanwhile a large quantity of antibodies can be fixed, finally sensitivity recognition can be directly performed on to-be-detected antigens through current signal changes, and therefore the electrochemical immunosensing interface has broad application prospects in immunoassay and electrochemical sensing fields.

Description

technical field [0001] The invention relates to a method for constructing an electrochemical immunosensing interface by using a conductive gel with an active site, and belongs to the field of immunodetection or electrochemical sensing. Background technique [0002] Electrochemical immunosensing is a combination of electrochemical analysis technology and immunodetection, and realizes the analysis of the analyte by using the specific recognition of antigens and antibodies and the change of electrical signals in electrochemistry. With its strong specificity and high sensitivity , good accuracy and easy operation, electrochemical immunosensing has shown great development prospects in the detection of cancer, heart disease and various metabolic diseases. How to improve its detection effect has always been one of the hot spots that researchers are most concerned about. Among them, since the recognition of the antibody and the antigen to be tested and the electron transfer between...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N33/531
CPCG01N27/26G01N27/30G01N33/531
Inventor 马姣毛晓慧丛虹霄张若茜
Owner TAIYUAN UNIV OF TECH
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