Method of constructing electrochemical immunosensing interface based on conductive gel with active sites
A conductive gel and active site technology, applied in the field of immunoassay or electrochemical sensing, can solve the problems of molecular skeleton binding sites, destroy Π conjugated network structure, hinder electron transport and other problems, and achieve rapid and gentle antibody chemistry. Fixed, broad application prospects, excellent conductivity effect
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[0021] The present invention will now be described in further detail in conjunction with the accompanying drawings and the following examples, but it should be understood that these examples are for illustrative purposes only, and should not be construed as limitations on the implementation of the present invention.
[0022] In this example, the supramolecular complex of vinyl ferrocene and monoaldehyde cyclodextrin is used to copolymerize with pyrrole to obtain a conductive gel, which is loaded on the working electrode and the antibody is immobilized to obtain an electrochemical immunosensing interface. Specific steps are as follows:
[0023] (1) Preparation of supramolecular complexes
[0024] Weigh 2.33 g of β-cyclodextrin and 0.523 g of 2-iodoxybenzoic acid in a 100 mL Erlenmeyer flask, add 20 mL of dimethyl sulfoxide to it, ultrasonically dissolve it for 2 min, react at room temperature for 24 h, and the reaction is complete After that, it was settled with cooling aceton...
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