Preparation method for bovine gamma interferon impedance type immunosensor based on zinc oxide nano-materials

A technology of zinc oxide nanometer and interferon gamma, which is applied in the direction of analyzing materials, material testing products, electrochemical variables of materials, etc., can solve the problems of small molecular weight of bovine interferon gamma, unsatisfactory sensitivity, and sensitivity to cytokines. , to reduce non-specific adsorption, improve immune response specificity and reaction efficiency, and achieve good biocompatibility

Active Publication Date: 2014-10-08
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, bovine gamma interferon detection commonly used biological analysis methods, these biological analysis methods have low sensitivity, laborious, time-consuming, and difficult to standardize
In addition, they are sensitive to buffers and other cytokines in the test sample and are susceptible to interference
Although enzyme-linked immunosorbent assays have also been established to detect bovine gamma interferon, these methods are also cumbersome, laborious, time-consuming, and the sensitivity is not satisfactory
[0004] Due to the small molecular weight of bovine gamma interferon and its extremely small amount in body fluids, the sensitivity of the current method for determining bovine gamma interferon is far from the actual clinical application.

Method used

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  • Preparation method for bovine gamma interferon impedance type immunosensor based on zinc oxide nano-materials
  • Preparation method for bovine gamma interferon impedance type immunosensor based on zinc oxide nano-materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of granular zinc oxide nanomaterials.

[0023] Weigh 0.5 g of submicron zinc oxide powder ( ZnO ) into a PTFE-lined stainless steel reactor with a capacity of 50 mL, add 40 mL of 20 % H 2 o 2 aqueous solution, stirred for 5 min. Then it was placed in an oven at 120 °C for 6 h, and the reaction system was naturally cooled to room temperature, filtered with suction, washed with deionized water several times, and finally dried in an oven at 80 °C for 10 h to obtain ZnO 2 White powder.

[0024] Weigh 0.5 g of ZnO prepared by hydrothermal reaction at 120 °C for 6 h 2 , placed in a 25 ml corundum crucible. Then it was put into a muffle furnace and heated at 300 °C for 6 h. After the reaction system was naturally cooled to room temperature, it was taken out to obtain a white ZnO powder.

[0025] (2) Under ultrasound, uniformly disperse the granular zinc oxide nanomaterials in deionized water to form a zinc oxide mixture with a zinc oxide content o...

Embodiment 2

[0033] (1) Preparation of rod-shaped ZnO nanomaterials.

[0034] Take 3.0 mmol of zinc nitrate (Zn(NO 3 ) 2 ·6H 2 O) Place in a PTFE-lined stainless steel reaction kettle with a volume of 100 mL, add 80 mL of absolute ethanol solution to make the filling degree of the Teflon reaction kettle reach 80%, stir at room temperature for 10 minutes, and weigh 30.0 mmol Sodium hydroxide (NaOH) was added to the above solution, stirred at room temperature for 1 h until completely dissolved. Then it was reacted at 120° C. for 12 h in an electric heating constant temperature blast drying oven, and the reaction system was naturally cooled to room temperature, then taken out, filtered with suction, cleaned several times with deionized water and dehydrated alcohol, and the sample was placed after suction filtration. Dried in an oven at 80° C. for 12 hours, and the rod-shaped zinc oxide nanomaterial of white powder can be obtained after drying.

[0035] (2) Uniformly disperse rod-shaped z...

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Abstract

The invention discloses a preparation method for a bovine gamma interferon impedance type immunosensor based on zinc oxide nano-materials, and belongs to the technical field of electrochemical immunoassay. A glassy carbon electrode is modified by the zinc oxide nano-materials with different morphologies and excellent performances, and a bovine gamma interferon antibody is fixed on the electrode, so that a novel electrochemical impedance type immunosensor is prepared. The novel electrochemical impedance type immunosensor can be applied to unmarked electrochemical immunoassay of bovine gamma interferon. The immunosensor does not need to be marked, is simple, quick, low in cost, high in sensitivity, good in reproducibility and good in stability, and can be used for early diagnosing of bovine tuberculosis and research of a bovine cellular immunity mechanism.

Description

technical field [0001] The invention belongs to the technical field of electrochemical immunoassay, in particular to the preparation technology of sensors. Background technique [0002] Tuberculosis is one of the most serious infectious diseases affecting the health and milk quality of dairy cows. Bovine gamma interferon (BoIFN-γ) is a type of glycoprotein secreted by T cells under the stimulation of a specific inducer with biological activities such as anti-virus, anti-tumor and immune regulation functions. It is the earliest discovery and the most concerned of cytokines. [0003] At present, the detection of bovine gamma interferon is commonly used by biological analysis methods, which are low in sensitivity, laborious, time-consuming, and difficult to standardize. In addition, they are sensitive to buffers and other cytokines in the assay sample and are susceptible to interference. Although enzyme-linked immunosorbent assays have also been established to detect bovine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N27/26
CPCG01N33/5438G01N33/6866
Inventor 杨占军秦坯芽陈祥
Owner YANGZHOU UNIV
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