Method for determining glucose in aqueous solution by using TiO2 nano-tube modified ito electrode

Abstract

The invention provides a method for determining the glucose in an aqueous solution by using a TiO2 nano-tube modified ito electrode. The method comprises: synthesizing TiO2 nano-tubes by using a hydrothermal method, grafting -OH on the surface, modifying an ito electrode simultaneously with the TiO2 nano-tubes and catechol by using an electrochemical method to form a composite film, modifying thesurface of the composite film with ferrocenecarboxylic acid, coating the surface of the outer layer of the ito electrode with glucose oxidase in a dropwise manner, and determining the glucose contentin the aqueous solution by using a chronoamperometry method. According to the present invention, the electrode is modified by coupling the TiO2 nano-tubes with the catalytic activity and the catechol,and the ferrocenecarboxylic acid synergistically acts as the electron mediator of glucose oxidase, such that the electron transfer and reaction signal during the glucose determination is enhanced, and the method has significant advantages in the determination of the trace sample.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to a 2 A nanotube-modified ito electrode is used to measure glucose in aqueous solution. Background technique [0002] The analysis of glucose will be a very popular research in the field of biomedicine in the future. At present, for the determination of glucose in the solution on the market, a glucose oxidase membrane detection device is used, that is, glucose oxidase can oxidize glucose under aerobic conditions to produce substances with certain chemical properties. The signal detector in the detection device The amount of substances with chemical properties can be detected, and the strength of the signal detected through analysis and research can be used to calculate the content of glucose in the sample. However, due to the traditional methods in the market for the determination of the stability of samples in dissolved oxygen and the elimination of interference from impurities i...

AI Technical Summary

Problems solved by technology

However, due to the traditional methods in the market for the determination of the stability of samples in dissolved oxygen and the elimination of interference from impurities in complex samples, there are great limitations in the linear range and lower limit of detection of the determination method.

Especially in the determination of trace samples, its limitations are more obvious

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