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Preparation method for constructing electrochemical immunosensor based on imitation mussel chemistry

An immunosensor and electrochemical technology, applied in the field of functional polymers, can solve the problems of inability to load signal molecules to provide enough effective sites, expand detection signals, etc., and achieve the effect of expanding the detection range and reducing the detection limit.

Pending Publication Date: 2019-12-20
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the study found that using nanomaterials alone as a carrier may not provide enough effective sites for the loading of signal molecules, failing to achieve the expected purpose of expanding the detection signal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Preparation of mussel-like chemically modified multi-walled carbon nanotubes (MWCNTs@PDA)

[0041] MWCNTs (100 mg) were dissolved in a mixed solution of ethanol (40 mL) and distilled water (30 mL), ultrasonically dispersed for 30 min, and then dopamine hydrochloride (80 mg) was added under magnetic stirring. After 10 minutes, Tris buffer (pH = 8.5, 50 ml) was added dropwise to the reaction system and reacted at room temperature for 24 hours. Finally, the product was obtained by washing with ethanol and distilled water and centrifuging.

Embodiment 2

[0042] Example 2: Preparation of Macroinitiator (MWCNTs@PDA-Br)

[0043] Dried MWCNTs@PDA (100 mg) was dissolved in anhydrous dichloromethane (DCM) (20 mL) and sonicated for 30 min. Dimethylaminopyridine (DMAP) (20 mg) and bromophenylacetic acid (BPA) (340 mg) were then added. Under stirring in an ice bath, dicyclohexylcarbodiimide (DCC) (330 mg) was dissolved in DCM (10 ml), and added dropwise to a round-bottomed flask with a constant pressure dropping funnel, and the temperature of the reaction system was gradually raised. to room temperature. After 24 hours, it was collected by centrifugation and washed several times with ethanol and distilled water, and finally the product was dried.

Embodiment 3

[0044] Example 3: Preparation of surface-initiated PET-ATRP functional polymer brushes (MWCNTs@PDA-PGMA)

[0045] GMA (6.04 ml, 45.6 mmol), MWCNTs@PDA-Br (50 mg), EY (15.8 mg, 0.0228 mmol), TEA (95 μl, 0.684 mmol), EBPA ( 40 μl, 0.228 mmol) and DMF (20 ml) in the reaction mixture. By filling the reactant with N 2 to achieve oxygen-free N 2 atmosphere. The mixture was then transferred to a magnetically driven stirring device containing LED flexible strips for LED irradiation (λ max = 460 nm), the reaction was carried out overnight at room temperature. The reaction was exposed to air and quenched in the dark. The reaction mixture was then dissolved in DMF and precipitated into a large amount of methanol solution. The lower precipitate was collected by centrifugation, washed with DMF and water, then dialyzed, and then freeze-dried to obtain the product.

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Abstract

The present invention discloses a method for constructing an electrochemical immunosensor by using the multi-walled carbon nanotube imitation mussel chemical modification to synthesize functional nano-composite probes. The method comprises: wrapping a polydopamine layer on the surface of a multi-walled carbon nanotube by using the imitation mussel excitation chemistry; after the polydopamine layeris grafted with the bromine initiator, using light-induced electron transfer-atom transfer radical polymerization and initiation on the surface of the polydopamine layer to form polymethylacrylic acid-glycidyl ester with a controllable molecular weight; using the amidogen to modify the polymethylacrylic acid-glycidyl ester to synthesize a functional nano-composite, and loading a large amount of electrochemically labeled anthraquinone dicarboxylic acid and ferrocenecarboxylic acid on the functional groups of the functional nano-composite to prepare two kinds of nano-composite probes for signalamplification; and finally, using the two functionalized nano-composite probes to prepare an electrochemical immunosensor, and performing electrochemical analysis and combined detection on two tumormarkers of the carcinoembryonic antigen and the alpha-fetoprotein.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical immunosensor, in particular to a method for designing multi-walled carbon nanotube nanocomposite probes based on mussel imitation chemistry and surface-induced atom transfer radical polymerization technology for the construction of electrochemical immunosensors The method belongs to the technical field of functional polymers. Background technique [0002] The detection of tumor markers (TMs) is an important method and means for clinical diagnosis of cancer. However, the content of TMs in the serum of early cancer patients is extremely low, so it is urgent to expand the detection signal and improve the sensitivity of detection. With the deepening of research, researchers have found that a tumor may secrete multiple tumor markers, so a single detection can no longer satisfy the status quo. Therefore, some highly specific and complementary tumor markers are often selected for joint dete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N27/26G01N27/48
CPCG01N27/26G01N27/48G01N33/574G01N33/68
Inventor 陈厚王娜柏良久王文香赵晓青范德超杨华伟魏东磊
Owner LUDONG UNIVERSITY
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