Method of using lipase to catalyze and synthesize mannose-6-laurate on line
A technology of laurate and mannose, applied in the field of lipase-catalyzed online controllable selective synthesis of mannose-6-laurate, which can solve the problems of low conversion rate and selectivity, long reaction time, etc. Effects of reaction time, reduced usage, high conversion and reaction selectivity
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Embodiment 1
[0026] Example 1: Synthesis of Mannose-6-laurate
[0027]
[0028] device reference figure 1 : Mannose (0.4mmol) was dissolved in 10mL tert-amyl alcohol: DMSO=4:1 (v / v) mixed solvent, vinyl laurate (4.4mmol) was dissolved in 10mL tert-amyl alcohol, and then packed in Ready-to-use in a 10mL syringe. 0.87g of lipase Lipozyme TLIM was evenly filled in the reaction channel of the microfluidic channel reactor, and driven by the PHD2000 syringe pump, the two reaction solutions were separated at 10.4 μL min -1 The flow rate enters the reaction channel through the "Y" joint for reaction, and the temperature of the reactor is controlled at 52°C by a water bath thermostat. The reaction solution flows continuously in the reaction channel for 30 minutes, and the reaction results are tracked and detected by thin-layer chromatography (TLC).
[0029] The reaction solution was collected online by the product collector, the solvent was distilled off under reduced pressure, and the column ...
Embodiment 2-5
[0033] Change the temperature of the microfluidic channel reactor, others are the same as in Example 1, and the reaction results are as shown in Table 1:
[0034] Table 1: Effect of Temperature on Reaction
[0035]
[0036] The results in Table 1 show that when the flow rate is 10.4 μL min -1 , when the reaction time is 30min, the conversion rate increases obviously with the increase of the reaction temperature. When the reaction temperature reaches 52°C, the conversion rate and selectivity of the reaction are the best. The decrease in enzyme activity leads to a decrease in the conversion rate and selectivity of the reaction, so the optimal reaction temperature of mannose laurate in the microfluidic microchannel reactor in the present invention is 52°C.
Embodiment 6-9
[0038] Change the substrate ratio of vinyl laurate and mannose in the microfluidic microchannel reactor to be 8: 1 (Example 6), 9: 1 (Example 7), 10: 1 (Example 8), 12: 1 (embodiment 9), other are with embodiment 1, and the result is as shown in table 2.
[0039] Table 2: Effect of Mannose to Vinyl Laurate Substrate Ratio on Reaction
[0040] Example
[0041] 9
[0042] The result of table 2 shows, along with the increase of reactant vinyl laurate, the conversion ratio of reaction also thereupon increases, and when substrate ratio is 11: 1, mannose is converted into mannose-6-palmitic acid mono ester, and the selectivity of the reaction is also very high, almost no other mannose esters are formed. Now if continue to increase the consumption of reactant vinyl laurate, the conversion rate and the selectivity of mannose-6-laurate will decrease instead. Therefore, the optimum substrate ratio for the reaction of the present invention is 11:1, under which rea...
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