Cephalosporin C acrylase and its vector and application

Abstract

The invention discloses a CPC acylation enzyme whose encode gene is DNA sequence shown as SEQ IN NO: 4 and DNA sequence having at least 80154000mology with the above DNA sequence. The invention also discloses carriers and transformants containing coding CPC acylation enzyme and the use of the enzyme. The enzyme has high expressing activity, high catalytic activity and high outcome tolerance to catalysis bottom object in high efficiency to prepare outcome 7-ACA.

Description

technical field

[0001] The invention belongs to the field of bioengineering, in particular to a cephalosporin C acylase, an expression vector containing the cephalosporin C acylase gene; and a method for preparing 7-aminocephalosporanic acid with the enzyme. Background technique

[0002] Cephalosporins, like penicillin, are a family of β-lactam broad-spectrum antibiotics, which play a bactericidal role by interfering with the synthesis of bacterial cell walls and accelerating the destruction of cell walls. The antibacterial part of cephalosporins is its mother nucleus 7-aminocephalosporanic acid (7-ACA), which is formed by the fusion of dihydrothiazide ring and β-lactam ring. It is a semi-synthetic cephalosporin Important intermediates of mycocin antibiotics. 7-ACA is mainly obtained from cephalosporin C (Cephalosporin C, CPC) by enzymatic or chemical cleavage and removal of the 7-position D-α-aminoadipyl side chain. The enzymatic method has the advantages of simple proces...

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