Fusion promoter pCLdb with both low temperature induction activity and potato tuber specific expression activity and construction method thereof

A low-temperature induction and potato block technology, which is applied in DNA preparation, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of low low-temperature induction activity, accumulation of reducing sugar in tubers, prolonging the processing time of potato tubers, etc. activity, increased expression activity, and small sequence differences
CN103361350BInactive Publication Date: 2015-04-22HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2015-04-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a fusion promoter pCLdb with both low temperature induction activity and potato tuber specific expression activity. The nucleotide sequence of the promoter is shown as SEQ ID NO. 1. According to the promoter, CRT (cathode ray tube) / DRE (direct recoding electronic) element flanking sequence of a gene promoter of the other low temperature induction promoter cor15b of arabidopsis and a fusion promoter pCL are taken as the basis to construct the fusion promoter pCLdb. The promoter pCLdb is constructed into a plant expression vector pBI121, the constructed vector is named as pCLdb-121, the constructed pCLdb-121 is used for converting potatoes, the expression strength of the promoter is evaluated by GUS (glucuronidase) enzyme activity detection, the GUS activity detection shows that the expression strength of the pCLdb is remarkably increased compared with that of the pCL, the expression strength of the pCLdb is higher than that of the promoter CaMV 35S commonly used at present, and the fusion promoter pCLdb can be used for improvement of potato tuber and low temperature character related gene engineering.
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Description

technical field

[0001] The invention belongs to the technical research field of eukaryotic gene expression regulation, and relates to a fusion promoter with both low-temperature induction activity and potato tuber-specific expression activity and a construction method thereof. Background technique

[0002] In order to prolong the processing time of potato tubers and prevent water evaporation, the harvested tubers are generally stored at low temperature. However, the "low temperature saccharification" that occurs during storage will lead to the accumulation of reducing sugars in the tubers and affect the quality of potato processing. This process involves a complex metabolic network, and the efficiency of traditional hybrid breeding is extremely low. Therefore, there are currently less than 10 varieties suitable for processing in the world. The improvement of this trait by transgenic technology is the main strategy. However, most of the genes involved in low-temperature sacc...

Claims

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