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Method for on-line synthesizing glucose-6-acetate catalyzed by lipase

A technology of glucose and lipase, which is applied in the field of online controllable and selective synthesis of glucose-6-acetate catalyzed by lipase, can solve the problems of low conversion rate and selectivity, long reaction time, etc., and achieve shortened reaction time, High reaction selectivity, high conversion effect

Active Publication Date: 2013-07-03
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, many domestic and foreign scholars have studied the enzyme-catalyzed synthesis of glucose esters in organic media, but this method often requires a long reaction time (24h), and the conversion rate and selectivity of the reaction are not high, so we A method for the selective synthesis of glucose-6-acetate catalyzed by lipase in a microchannel reactor was studied, aiming to find an efficient and environmentally friendly on-line controllable selective synthesis method of glucose-6-acetate monoester

Method used

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  • Method for on-line synthesizing glucose-6-acetate catalyzed by lipase
  • Method for on-line synthesizing glucose-6-acetate catalyzed by lipase
  • Method for on-line synthesizing glucose-6-acetate catalyzed by lipase

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Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Synthesis of Glucose-6-Acetate

[0028]

[0029] device reference figure 1 : Glucose (0.4mmol) was dissolved in 10mL of tert-amyl alcohol: DMSO=4:1 (v / v) mixed solvent, vinyl acetate (8.0mmol) was dissolved in 10mL of tert-amyl alcohol, and then filled into 10mL syringes respectively in spare. 0.87g of lipase Lipozyme TLIM was evenly filled in the reaction channel of the microfluidic channel reactor, and driven by the PHD2000 syringe pump, the two reaction solutions were separated at 10.4 μL min -1 The flow rate enters the reaction channel through the "Y" joint for reaction, and the temperature of the reactor is controlled at 52°C by a water bath thermostat. The reaction solution flows continuously in the reaction channel for 30 minutes, and the reaction results are tracked and detected by thin-layer chromatography (TLC).

[0030] The reaction solution was collected online by the product collector, the solvent was distilled off under reduced pressure, an...

Embodiment 2-5

[0034] Change the temperature of the microfluidic channel reactor, others are the same as in Example 1, and the reaction results are as shown in Table 1:

[0035] Table 1: Effect of Temperature on Reaction

[0036]

[0037] The results in Table 1 show that when the flow rate is 10.4 μL min -1 , when the reaction time is 30min, the conversion rate increases obviously with the increase of the reaction temperature. When the reaction temperature reaches 52°C, the conversion rate and selectivity of the reaction are the best. The reduction of enzyme activity leads to a reduction in the conversion rate and selectivity of the reaction, so the optimum reaction temperature of glucose acetate in the microfluidic microchannel reactor in the present invention is 52°C.

Embodiment 6-10

[0039] Change the substrate molar ratio of vinyl acetate and glucose in the microfluidic microchannel reactor to be 16: 1 (Example 6), 17: 1 (Example 7), 18: 1 (Example 8), 19: 1 (Example 9), 21:1 (Example 10), 22:1 (Example 11), others are the same as Example 1, and the results are shown in Table 2.

[0040] Table 2: Effect of glucose to vinyl acetate substrate ratio on the reaction

[0041] Example Vinyl acetate:glucose Conversion rate[%] selectivity [%] 6 16∶1 65 100 7 17∶1 75 100 8 18∶1 84 100 9 19∶1 90 100 1 20∶1 99 100

[0042] 10 21∶1 98 99 11 22∶1 98 98

[0043] The results in Table 2 show that with the increase of the reactant vinyl acetate, the conversion rate of the reaction also increases. When the substrate ratio is 20:1, the conversion rate and selectivity of the reaction are optimal, and the glucose has been quantitatively comple...

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Abstract

The invention discloses a method for on-line synthesizing glucose-6-acetate catalyzed by lipase. According to the method, glucose and vinyl acetate with a molar ratio being 1 : 16-22 are used as raw materials; 0.5-1.0 g of the lipase (Lipozyme TLIM) is used as a catalyst and a mixed solvent formed by tert-amyl alcohol and DMSO is used as a reaction solvent. The lipase (Lipozyme TLIM) is uniformly filled in a reaction channel of a microfluidic channel reactor, wherein the inner diameter of the reaction channel of the microfluidic channel reactor is 0.8-2.4 mm, and the length of the reaction channel is 0.5-1.0 m; the raw materials and the reaction solvent are continuously introduced into the reaction channel for an acylation reaction, wherein the temperature of the acylation reaction is controlled at 40-55 DEG C and the time of the acylation reaction is 15-35 min; a reaction liquid is collected on-line; and the glucose-6-acetate is obtained after the reaction liquid is subjected to conventional post-treatment. The method provided by the invention has the advantages of short reaction time, high selectivity and high yield.

Description

(1) Technical field [0001] The invention relates to a lipase-catalyzed online controllable selective synthesis of glucose-6-acetate. (2) Background technology [0002] Sugar ester is an excellent non-ionic surfactant, which uses natural renewable resource sugar widely existing in nature as raw material, and has broad development prospects. Today's surfactant industry is developing in the direction of green chemistry, which gradually requires non-toxic products, regenerated reaction raw materials, and green reaction processes. As an excellent natural surfactant, sugar esters meet the two requirements of green chemistry: non-toxic products and renewable raw materials, but the reaction process still needs to be further green. [0003] At present, the sugar esters commercially produced at home and abroad are all synthesized by chemical methods. There are many shortcomings in chemical methods, mainly including: the conditions in the reaction process are severe, side reactions su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02
Inventor 罗锡平杜理华张航诚顾生妹胡玉婷
Owner ZHEJIANG UNIV OF TECH
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