High temperature-resistant yeast strain for producing pyruvic acid and use thereof

A technology of pyruvic acid and yeast, applied in the biological field, can solve the problems of difficulty in meeting the needs of sustainable economic and social development, protection of the unfavorable environment of pyruvate, and the like

Inactive Publication Date: 2013-06-12
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical production of pyruvic acid is not conducive to the protection of the environme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1 Obtaining of Torulopsis glabrata TIB-G90 CGMCC No5434

[0010] Torulopsis glabrata 3672 CGMCC No0879 was inoculated in the adaptive evolution medium, the medium pH was 5.5, and contained the following components in mass percentage: glucose 10%, beef extract 3%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.05%, CaCO 3 4%. After culturing at 37°C and 220r / min for 12 hours in a 50ml shake flask containing 10mL of medium, it was then inoculated in a fresh same medium with an OD600 value of 0.2, and subcultured in the same way. By analogy, after adapting the strain to a stable growth environment at 37°C, use the same medium and culture conditions to gradually increase the growth temperature, and carry out adaptive acclimation until the temperature reaches 40°C, so that it can still grow stably, and the growth rate is the same as the starting point. The growth amount of the strains was basically the same at 30° C., so that Torulopsis glabrata TIB-G90 CGMCC No5434 was obtain...

Embodiment 2

[0011] Example 2 30°C Fermentation Comparison of Torulopsis glabrata 3672 CGMCC No0879 and (Torulopsis glabrata) TIB-G90 CGMCC No5434

[0012] Toruula glabrata 3672 CGMCC No0879 and TIB-G90 CGMCC No5434 were respectively inoculated in the seed culture medium, which had a pH of 5.5 and contained the following components in mass percent: glucose 10%, soybean peptone 3%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.05%, CaCO 3 3%, after 24 hours of cultivation at 30°C and 220r / min in a 50ml shake flask containing 10mL, take 5mL seed liquid and inoculate it into a 500mL shake flask containing 50mL fermentation medium, the pH of which is 5.5 , containing the following mass percentage components: glucose 10%, (NH 4 ) 2 SO 4 3%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.05%, Vitamin B10.00001%, Vitamin B60.0002%, Niacin 0.0004%, Biotin 0.00002%, CaCO 3 4%. Cultivate at 30° C. and 220 r / min for 36 hours, and measure the growth of the bacteria and the content of pyruvate and ethanol in the fermen...

Embodiment 3

[0013] Example 3 40°C Fermentation Comparison of Torulopsis glabrata 3672 CGMCC No0879 and TIB-G90 CGMCC No5434

[0014] Toruula glabrata 3672 CGMCC No0879 and TIB-G90 CGMCC No5434 were respectively inoculated in the seed culture medium, which had a pH of 5.5 and contained the following components in mass percent: glucose 10%, soybean peptone 3%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.05%, CaCO 3 3%, after 12 hours of cultivation at 40°C and 220r / min in a 50ml shake flask containing 10mL, take 5mL seed solution and inoculate it into a 500mL shake flask containing 50mL fermentation medium, the pH of which is 5.5 , containing the following mass percentage components: glucose 10%, (NH 4 ) 2 SO 4 3%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.05%, Vitamin B10.00001%, Vitamin B60.0002%, Niacin 0.0004%, Biotin 0.00002%, CaCO 3 4%. Cultivate at 40°C and 220r / min for 24 hours, and measure the growth of the bacteria and the content of pyruvate and ethanol in the fermentation broth by convent...

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Abstract

The invention discloses a high temperature-resistant yeast strain for producing pyruvic acid and a use thereof. Pyruvic acid is an important organic acid in metabolism. The high temperature-resistant yeast strain for producing pyruvic acid has wide purposes. The high temperature-resistant yeast strain is high temperature-resistant Torulopsis glabrata TIB-G90 CGMCC No. 5434 and is screened by a high-temperature adaptive evolution method. The high temperature-resistant Torulopsis glabrata TIB-G90 CGMCC No. 5434 can grow well at a temperature of 40-45 DEG C and does not influence an accumulation amount of pyruvic acid in a fermentation broth. The high temperature-resistant yeast strain can reduce a temperature reduction cost in production and has wide industrial application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pyruvic acid-producing high-temperature-resistant yeast, Torulopsis glabrata TIB-G90 CGMCC No5434, and a method for fermenting and producing pyruvic acid with the fungus. Background technique [0002] Pyruvate is one of the most important chemical intermediates, widely used in pharmaceutical, food, chemical, agrochemical and scientific research fields. The production of pyruvic acid by chemical method is not conducive to the protection of the environment, and it is difficult to meet the needs of sustainable economic and social development. Years of research and development at home and abroad have obtained a series of strains with pyruvate production exceeding 50g / L, and some even exceeding 100g / L, making the industrialization of pyruvate biomanufacturing possible (Qiang Hua, Chen Yang, kazuyuki Shimizu: J Biosci Bioeng, 1999 ; Li Y, Chen J, Lun SY: Appl Microbiol Biote...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/40C12R1/88
Inventor 王钦宏赵博涂然贺鹏江宁
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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