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Method for improving pyruvic acid yield via fermentation process by using urea as nitrogen source and application

A fermentation method and pyruvic acid technology are applied in the field of fermentation engineering to achieve the effects of improving production intensity, increasing yield and shortening fermentation cycle

Inactive Publication Date: 2011-12-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the effect of different nitrogen utilization levels, especially urea, on pyruvate production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Fermentation condition is the same as the control example, changes the nitrogen source into two kinds of compound nitrogen sources of ammonium chloride and urea in the fermentation medium, ammonium chloride: urea (1: 1) is 6.2: 3.5g / L, compound nitrogen The nitrogen content in the source is equivalent to the nitrogen content of 7g / L urea.

[0020] It was found that: ①The growth of the bacteria was accelerated, and the dry weight of the bacteria was 16.5g / L when the fermentation was 72 hours; the increase (15.9g / L) was 2.5%; ②When the fermentation was carried out to 72 hours, the sugar consumption rate of the bacteria increased by 13.1%; The fermentation period was shortened from 85h to 79h, with a range of 7.1%; ④ At the end of fermentation, the highest pyruvate concentration was 82.1g / L, an increase of 3.0%; ⑤ The production intensity of pyruvate fermentation increased by 10.6%.

Embodiment 2

[0021] Embodiment 2: The fermentation conditions are the same as the control example, the nitrogen source in the fermentation medium is changed to urea, and the amount of urea added is 7g / L.

[0022] It was found that: ①The growth of the bacteria was accelerated, and the dry weight of the bacteria was 17.8g / L when the fermentation was 72 hours; the increase was (15.9g / L) 11.9%; ②When the fermentation was carried out to 72 hours, the sugar consumption rate of the bacteria increased by 20.2%; The fermentation period was shortened from 85h to 72h, with a range of 15.3%; ④ at the end of fermentation, the highest concentration of pyruvate was 88.7g / L, an increase of 11.3%; ⑤ at the end of fermentation, the intensity of pyruvate production increased by 30.9%.

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Abstract

The invention discloses a method for improving pyruvic acid yield via a fermentation process by using urea as a nitrogen source and belongs to the technical field of fermentation engineering. In the invention, multi-vitamin auxotrophic torulopsis glabrata CCTCCM202019 is used as a producing strain, urea is used in place of ammonium chloride as a unique nitrogen source, the initial added amount per liter is 7 grams, and batch fermentation culture is performed in a laboratory 7-liter fermentation tank. The method can effectively improve pyruvic acid yield (11.9 percent) and production strength (30.9 percent) and greatly reduce a fermentation period (20.6 percent); the achievements are successfully tested in a 30-liter fermentation tank; and the method has a bright industrial application prospect.

Description

technical field [0001] The invention relates to a method for using urea as a nitrogen source to increase the yield of pyruvic acid produced by a fermentation method, and belongs to the technical field of fermentation engineering. The invention also relates to the application of the above method in the production of pyruvic acid by fermentation, which belongs to the field of fermentation engineering. Background technique [0002] Pyruvate, also known as 2-Oxopropanic acid, α-Ketopropionic acid or Acetylformic acid, is a colorless to light yellow liquid with acetic acid aroma and Pleasant sour taste, is one of the most important α-oxocarboxylic acids. Pyruvate not only plays a very important role in bioenergy metabolism, but also is a precursor for the synthesis of many useful compounds such as dopamine and L-tyrosine. Therefore, it has a wide range of applications in industrial and scientific research such as pharmaceuticals, daily chemicals, and agricultural chemicals. ...

Claims

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Application Information

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IPC IPC(8): C12P7/40C12R1/645
Inventor 刘立明杨松鑫陈修来汪军陈坚
Owner JIANGNAN UNIV
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