Method for increasing yield of pyruvic acid

A pyruvic acid and yield technology, applied in the field of fermentation engineering, can solve problems such as high application range, decreased organic acid capacity and speed, and increased osmotic pressure, and achieve the effects of low professional quality requirements, promoting strain growth, and improving acid resistance.

Inactive Publication Date: 2013-08-07
JIANGNAN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] Generally speaking, in the process of organic acid fermentation, in order to maintain the pH of the fermentation system in the optimum range, a certain amount of alkaline substances such as NaOH is added, and with the addition of NaOH, the osmotic pressure of the fermentation broth is continuously increased; When the osmotic pressure in the solution reaches a certain concentration, the ability and speed of the cells to further accumulate organic acids will gradually decrease, making it difficult to reach the maximum theoretical yield.
Although the combination of bioreaction and product separation technology can improve the performance and efficiency of bioreaction process, the scope of application and high equipment cost of this method limit its application in large-scale industrial production

Method used

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  • Method for increasing yield of pyruvic acid
  • Method for increasing yield of pyruvic acid
  • Method for increasing yield of pyruvic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 strain

[0015] A strain of Torulopsis glabrata with quadruple vitamin auxotrophy, constitutive reduction of pyruvate decarboxylase and large accumulation of pyruvate CCTCC No.M202019, taxonomically named Torulopsis glabrata, a strain selected for the laboratory , the strain has applied for a Chinese patent, the application number is 02113142.2, and the publication number is CN1392246A.

Embodiment 2

[0016] Embodiment 2 produces pyruvic acid by fermentation

[0017] T.glabrata CCTCC M202019 was first transferred to a slant and cultured in a 30°C incubator for 16 hours to perform live activities. The activated single colony was picked and inoculated into the seed medium, and cultured at 30° C. and 200 rpm for 24 hours. Transfer the seeds to the fermentation medium with 10% inoculation amount, add aspartic acid to make the final concentration 1.5g / L, set the pH to 5.5 and 4.5 respectively, and adjust the pH with 8mol / L NaOH or 2mol / L HCl agent, cultivated at 30°C, 200rpm for 56h. After the fermentation, the cell concentration and pyruvate production were measured, and the results are shown in Table 1.

[0018] The seed medium (g / L) is: glucose 30, peptone 10, KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.5; add agar 20g / L to the slant medium, pH 5.5.

[0019] Table 1 Determination of bacterial cell concentration and pyruvate concentration

[0020]

[0021]

[0022] Under the c...

Embodiment 3

[0023] Embodiment 3 produces pyruvic acid by fermentation

[0024] Add aspartic acid so that the final concentration is 0.5g / L, and others are the same as in Example 2.

[0025]

[0026] When the concentration of aspartic acid added is 1g / L,

[0027]

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Abstract

The invention discloses a method for increasing yield of pyruvic acid, belonging to the fermentation engineering field. The method is as follows: when Torulopsis glabrata (CCTCC NO: M202019) is used to ferment and produce pyruvic acid, aspartic acid is added to increase the acid stress resistance of Torulopsis glabrata. When the pH values are 5.5 and 4.5, the yields of pyruvic acid are separately55.8g / L and 30.4g / L which are separately increased by 12.4% (49.6g / L) and 48.4% (20.5g / L) than the control groups without aspartic acid; and the method has better technical effect and wide application prospect.

Description

technical field [0001] The present invention relates to a method for increasing the production of pyruvate, especially a method for adding energy-assisted substrate aspartic acid during cultivation to increase intracellular ATP levels, enhance the acid tolerance of bacterial strains, protect cell growth, and increase the production of pyruvate , belonging to the field of fermentation engineering. Background technique [0002] The production of pyruvate by fermentation has been studied since the 1950s, and many achievements have been made. Using the principles of fermentation process optimization and metabolic engineering, aiming at unifying pyruvate with high yield, high yield and high production intensity, the fermentation process of pyruvate produced by multiple auxotrophic strain Torulopsis glabrata was carried out. A large number of research work such as optimization and transformation have led to a substantial increase in the production of pyruvate. With increased pyr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/50C12R1/85
Inventor 刘立明汪军陈坚
Owner JIANGNAN UNIV
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