Industrial production and fermentation technology of pyruvic acid

A fermentation process, pyruvate technology, applied in the direction of fermentation, microorganisms, fungi, etc., can solve the problems of low yield, different range of process control points, long fermentation cycle, etc., achieve high yield, ensure product quality, quality and The effect of process stability

Inactive Publication Date: 2017-06-13
TONGLIAO HUANGHELONG BIOENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] (3), 2012, Volume 31, Issue 4 "China Brewing". Torulopsis glabrata adapted to evolution to improve pyruvate production. Zhao Bo et al.; there are certain technical defects in the above references: first, the fermentation cycle is long, The yield is low; second, the degradation or transformation of pyruvic acid in the fermentation reality has not been effectively solved; third, the changes in the fermentation process parameters of the pilot test and the large-scale fermentation tank with 150 cubic meters of mass production are different , and the range of process control points is also different, so as to realize industrial mass production

Method used

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  • Industrial production and fermentation technology of pyruvic acid
  • Industrial production and fermentation technology of pyruvic acid
  • Industrial production and fermentation technology of pyruvic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Pyruvate industrial mass production fermentation process, the specific process steps are as follows:

[0051] (1) Plate culture:

[0052] A. Plate medium (g / L): Glucose syrup 3, soybean peptone 3, beef extract peptone agar 3, calcium carbonate 0.5, dissolved in raw water, natural pH, sterilized at 121°C for 15 minutes for use;

[0053] B. Torulopsis glabrata was cultured in the plate culture medium at 30°C in an inverted culture dish for 20 hours;

[0054] (2) Eggplant bottle culture:

[0055] a. Eggplant bottle medium (g / L): glucose syrup 13, soybean peptone 11, beef extract peptone agar 10, calcium carbonate 2, ammonium chloride 0.3, dissolved in raw water, natural pH, sterilized at 121°C for 15 minutes for use;

[0056] b. Strictly follow the aseptic operation in the sterile room, pick a single colony from the preservation plate and spread it on the solid medium of the eggplant bottle;

[0057] c. Culture conditions: 30°C, after 20 hours of culture;

[0058] d. S...

Embodiment 2

[0078] The eggplant bottles stored at 1°C in the refrigerator were used for shake flask inoculation. Shake flask medium (g / L): glucose syrup 17.5, soybean peptone 14.5, calcium carbonate 3.0, ammonium chloride 0.55, magnesium chloride 0.38, dipotassium hydrogen phosphate 0.28, Dissolve the raw water and adjust the initial pH of the medium to 5.5 with HCL at a concentration of 1.5mol / L, and sterilize at 121°C for 15 minutes for later use;

[0079] II. 20 to 30 3000ml or 5000ml triangular flasks or steel cylinders. Use a sterilized inoculation shovel in the sterile room to scoop out the bacteria from the cultured eggplant bottles and transfer them to 500ml triangular flasks or steel cylinders; The inoculum volume is 600ml / bottle or 1000ml / bottle; the cultivation temperature is 30℃, and the cultivation time is 24h;

[0080] Primary seed culture:

[0081] ① Primary seed medium (g / L): Glucose syrup 21, soybean peptone 17.5, calcium carbonate 6.8, ammonium chloride 1.8, magnesium c...

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Abstract

The invention discloses an industrial production and fermentation technology of pyruvic acid. The technology comprises plate cultivation of torulopsis glabrata, tomato bottle cultivation, shake-flask cultivation and first-level seed tank cultivation for industrial production and fermentation technology of a fermentation tank of 150 cubic meters, and determination of specific components and ratios of a culture medium. The process blank of industrial production and fermentation of the pyruvic acid, especially fermentation and production suitable for the large fermentation tank of 150 ic metes is filled; the fermentation period is shortened to 56h; relatively high yield is also obtained; internal friction and conversion of a strain on saturated pyruvic acid of a fermentation liquid are effectively solved; and the yield and quality of a product are ensured.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a pyruvate industrial mass-production fermentation process suitable for a 150-cubic large-scale fermentation tank. Background technique [0002] As one of the important organic acids, pyruvic acid, also known as 2-oxypropionic acid, α-ketopropionic acid or acetyl formic acid, is a colorless to pale yellow liquid with acetic acid aroma and pleasant sour taste, and is the most important α- One of the oxocarboxylic acids. Pyruvate not only plays a very important role in bioenergy metabolism, but also is a precursor for the synthesis of many useful compounds. Therefore, it is widely used in industrial and scientific research such as pharmaceuticals, daily chemicals, agricultural chemicals and food . [0003] At present, compared with pyruvic acid produced by chemical synthesis, the production of pyruvic acid by fermentation has the advantages of low cost and high qua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12N1/16C12R1/88
CPCC12N1/16C12P7/40
Inventor 许友民马辉石光东
Owner TONGLIAO HUANGHELONG BIOENG
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