The invention provides a high efficiency fluidization upward flow multilayer composite filter material biological filter tank coupled with ozonation and a water treatment method thereof, relating to a biological filter tank and a water treatment method using the biological filter tank. The biological filter tank is composed of an upper filter material, a medium filter material, a lower filter material and a supporting layer, wherein the upper filter material is active carbon, anthracite or biological ceramsite, the medium filter material is modified zeolite, natural zeolite or synthetic zeolite and the lower filter material is a phosphorus functional material. The filter tank is characterized by fast start, a complex and stable biological phase, great biomass, high activity, strong impact resistance, a filter material expansion rate of 0% to 50%, small resistance at the head of the filter tank, high mass transfer efficiency of materials, oxygen and pollutants, a high utilization rate of filter layers and filter materials and small possibility of occurrence of hardening of the filtering materials. The filter tank is applicable to treatment of raw water in which the concentration of water source phosphorus, ammonia nitrogen and organic pollutants fluctuates and can stably operate at a low temperature.